Sequencing-free whole-genome spatial transcriptomics at single-molecule resolution.
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Published Erratum↗
Biomedical subjects
Miao Liu
Publications and source records attributed to Miao Liu.
Multi-omics panorama of glaucoma: Pathogenesis, biomarkers, and novel therapeutic strategies.
Glaucoma is a group of irreversible, blinding eye diseases characterized by progressive loss of retinal ganglion cells, leading to gradual visual field defects that severely impact patients' quality of life. Its complex pathophysiological mechanisms remain incompletely understood, limiting the development of early diagnostic and effective therapeutic strategies. Advances in omics technologies have provided new insights into elucidating the pathophysiology of glaucoma. We summarize specific alterations in genomics, transcriptomics, proteomics, metabolomics, epigenomics, and microbiomics associated with glaucoma. We emphasize the systematic analysis of disease mechanisms, identification of clinically applicable biomarkers, and discovery of novel therapeutic targets through the integration of these data. This approach paves new pathways for glaucoma subtype diagnosis and personalized treatment, while also outlining future research directions and challenges.
Humans↗
Clinical and Multiorgan Proteomics Characteristics of the Diverse Fatal Phase in Super Elderly Patients With SARS-CoV-2 Infection: A Descriptive Study.
This study aims to identify the risk factors associated with clinical outcomes and the proteomic changes in organs related to fatal SARS-CoV-2 infection within the super-elderly population. This retrospective analysis included all elderly individuals with COVID-19 admitted to the Second Medical Center of PLA General Hospital from December 2022 to January 2023. The follow-up period ended on March 30, 2023. During this time, epidemiological, demographic, laboratory, and outcome data were analyzed descriptively. Proteomic sequencing was performed on super-elderly patients who died from COVID-19 at different stages of the disease. A total of 352 elderly COVID-19 patients, with a mean age of 89.84 ± 8.54 years, were included in this study. During a median follow-up period of 98 days, 79 patients died. Deceased patients were older and more likely to have cardiovascular and cerebrovascular diseases, with a lower prevalence of lipid-lowering therapy. The number of deaths in the acute and post-acute phases were 34 and 45, respectively. Proteomics data suggest that the immune systems of patients who died in the acute phase underwent a more rapid and severe onslaught. Patients in the post-acute phase showed higher levels of viral genome replication and a more robust immune response. However, the over-activation of the immune system led to systemic organ dysfunction. Effective management of comorbidities may improve the prognosis of COVID-19 in super-elderly patients. The continuous replication of the SARS-CoV-2 virus and its subsequent impact on the immune system are critical determinants of survival time in this demographic.
Humans↗
Large continuous, transparent and oriented self-supporting films of layered double hydroxides with tunable chemical composition.
Highly ordered transparent self-supporting films of layered double hydroxides (LDHs) with a size of the order of cm2 have been obtained by a simple method; the chemical composition of both the layers and interlayers can be readily tuned, as demonstrated by the preparation of [ZnAl-NO3] LDH, [NiAl-NO3] LDH and [Tb(EDTA)]- intercalated-ZnAl LDH films.
Journal Article↗
A taxonomic revision of the insect biocontrol fungus Aschersonia aleyrodis, its allies with white stromata and their Hypocrella sexual states.
A revision of a monophyletic group of Hypocrella species and their Aschersonia anamorphs with white effuse stromata is presented. In addition to taxon descriptions, distributions, and nomenclature, a synoptic key and a molecular phylogenetic analysis are also provided. A new holomorph, Hypocrella rhombispora sp. nov., is described. This study presents a revisionary treatment of Aschersonia aleyrodis (teleomorph: Hypocrella libera) and its allies. These fungi parasitize whiteflies and are promising candidates for whitefly biological control. Four species of Aschersonia and their Hypocrella teleomorphs are treated in detail: Aschersonia aleyrodis/Hypocrella libera; A. andropogonis/H. andropogonis, A. placenta/H. raciborskii, and A. sp./H. rhombispora sp. nov. A synoptic key including these and six other morphologically similar species is presented to facilitate identification in the field and laboratory. Phylogenetic analyses of partial DNA sequences from three genes (LSU, mtSSU, and RPB2) suggest that Aschersonia species with effuse white stromata form a monophyletic group of whitefly pathogens. Phylogenetically informative characters in the group include the colour and shape of the stromata, the arrangement of tubercles containing perithecia, the arrangement of conidial masses on the stromata, and the shape of conidia and part spores.
Hypocreales↗
Osteosarcoma: evaluation of information on the internet.
The information about osteosarcoma on the existing websites was examined. The purpose of this study was to evaluate the quality of Internet information available to patients on the topic of osteosarcoma. The secondary purpose was to rank the identified websites with respect to the caliber of relevant information provided by the websites sponsors' identity. A great many of people in the world "surf" the World Wide Web, searching for medical information. The information on the Internet varies dramatically in terms of content and quality for lack of uniformed standard with respect to the medical publications on the Internet. Five search engines searched the search terms "osteosarcoma." The first 25 links displayed by each engine were evaluated for a theoretical total of 125 websites. According to the information context, sponsor identity we evaluated each website. An information quality score of 0 to 26 was generated for each site. A score of 20 or greater was thought to be "high-quality" information website. Eighty-nine unique websites were identified, among which only 8 (9%) scored 20 or more on the information quality score; 32 scored 10 or less. The overall mean information quality score was 11.5. The websites with highest mean scores were academic organization and affiliated hospitals of university. The quality of Internet information on osteosarcoma is variable. Less than 10% of relevant websites were of high information value. The rank list of high quality websites from our information quality score should provide useful information of osteosarcoma.
Humans↗
Repairing articular cartilage defects with tissue-engineering cartilage in rabbits.
OBJECTIVE: To investigate the effect of cancellous bone matrix gelatin (BMG) engineered with allogeneic chondrocytes in repairing articular cartilage defects in rabbits. METHODS: Chondrocytes were seeded onto three-dimensional cancellous BMG and cultured in vitro for 12 days to prepare BMG-chondrocyte complexes. Under anesthesia with 2.5% pentobarbital sodium (1 ml/kg body weight), articular cartilage defects were made on the right knee joints of 38 healthy New Zealand white rabbits (regardless of sex, aged 4-5 months and weighing 2.5-3 kg) and the defects were then treated with 2.5% trypsin. Then BMG-chondrocyte complex (Group A, n=18), BMG (Group B, n=10), and nothing (Group C, n=10) were implanted into the cartilage defects, respectively. The repairing effects were assessed by macroscopic, histologic, transmission electron microscopic (TEM) observation, immunohistochemical examination and in situ hybridization detection, respectively, at 2, 4, 8, 12 and 24 weeks after operation. RESULTS: Cancellous BMG was degraded within 8 weeks after operation. In Group A, lymphocyte infiltration was observed around the graft. At 24 weeks after operation, the cartilage defects were repaired by cartilage tissues and the articular cartilage and subchondral bone were soundly healed. Proteoglycan and type II collagen were detected in the matrix of the repaired tissues by Safranin-O staining and immunohistochemical staining, respectively. In situ hybridization proved gene expression of type II collagen in the cytoplasm of chondrocytes in the repaired tissues. TEM observation showed that chondrocytes and cartilage matrix in repaired tissues were almost same as those in the normal articular cartilage. In Group B, the defects were repaired by cartilage-fibrous tissues. In Group C, the defects were repaired only by fibrous tissues. CONCLUSIONS: Cancellous BMG can be regarded as the natural cell scaffolds for cartilage tissue engineering. Articular cartilage defects can be repaired by cancellous BMG engineered with allogeneic chondrocytes. The nature of repaired tissues is closest to the normal cartilage. Local administration of trypsin can promote the adherence of repaired tissues to host tissues. Transplantation of allogeneic chondrocytes has immunogenicity, but the immune reaction is weak.
Animals↗
[Establishment of method for cytochrome P450 of earthworms (Eisenia fetida) as a biomarker].
Earthworm (Eisenia fetida) was chosen as test soil fauna to establish the method for determination of cytochrome P450 content. By means of thoroughly washing with salt solution, changing centrifugal acceleration and dissolving microsomal membranes of earthworms with sodium cholate, the determination of cytochrome P450 was performed. Base on the method, dynamic relationships of dose-response for cytochrome P450 contents in earthworm and phenanthrene concentrations were conducted by contact filter paper and soil contaminated with phenanthrene. Results indicate that cytochrome P450 are covered up by interferential material in earthworm, which makes the classic peak lag and appear at 455 - 457nm in the CO difference spectrum with a spectrophotometer. Through increasing centrifugal acceleration and adding reagent of solubilization, the interference is removed mostly and the classic peak of P450 returns to appear at 450nm +/- 1nm. The exposure tests of phenanthrene with contact filter paper (10(-6), 10(-5), 10(-4), 10(-3) and 10(-2) mg x mL(-1)) and soil (1, 2, 4 and 8 mg x kg(-1)) reveal that the dose-response relationships exist between the pollutant and cytochrome P450. Soil tests of different exposure durations (1, 3, 7, 14 and 28d) show the effects of different phenanthrene concentration on earthworm cytochrome P450 contents are to activate (7d) and to inhibit (14d and 28d), and the content of P450 is 0.99 - 1.41 and 0.77 - 0.88 (p < 0.05) times of control respectively. From this study, cytochrome P450 content of earthworm has the merit of simpleness, speediness and economy for determination, and it could be used as a sensitive biomarker for monitoring the exposure of sublethal pollution in terrestrial ecosystem.
Animals↗
Hypocrella zhongdongii sp. nov., the teleomorph of aschersonia incrassata.
A new Hypocrella species with white pulvinate stromata collected in Puerto Rico and Costa Rica is described as H. zhongdongii sp. nov. Morphological and molecular evidence confirms that the new species of Hypocrella is the teleomorph of Aschersonia incrassata. It most closely resembles H. andropogonis; both A. incrassata and A. andropogonis are common yellow-spored species. The relationships of H. zhongdongii with other species in the genus are elucidated through phylogenetic analyses of three different genetic loci (LSU, RPB2, and mtSSU). Our analysis also sheds light on current subgeneric concepts in Aschersonia, in which the presence or absence of conidiomatal paraphyses is a major character to separate the genus into two subgenera. The present phylogenetic tree suggests that paraphyses have been lost or gained multiple times during evolutionary history, and do not define monophyletic groups.
Claviceps↗
A new species of Hypocrella, H. macrostroma, and its phylogenetic relationships to other species with large stromata.
Two specimens of a new species of Hypocrella with large stromata were collected in Bolivia and Costa Rica. The morphology of the new species, H. macrostroma sp. nov., was compared with that of other species with large stromata, i.e. H. africana, H. gaertneriana, and H. schizostachyi. In addition, phylogenetic analyses of partial sequences from three genes, large subunit nuclear ribosomal DNA (LSU), translation elongation factor 1-alpha (EF1-alpha), and RNA polymerase II subunit 1 (RPB1), were conducted to determine the relationships of the new species to other species of Hypocrella/Aschersonia. Phylogenetic analyses show that H. macrostroma belongs to a strongly supported clade that includes H. africana, H. schizostachyi, and Aschersonia insperata, whereas other Hypocrella species belong to two sister clades. Hypocrella macrostroma is described and illustrated, and a lectotype is designated for H. gaertneriana.
Animals↗
[Study on classification and treatment of vulvovaginal candidiasis].
OBJECTIVE: To determine the clinical manifestations of vulvovaginal candidiasis (VVC) and to study the mycologic eradication rate of different miconazole treatment courses for VVC. METHODS: Three hundred cases of VVC were recruited. The Candidas were cultured. A prospective and randomized study was performed to compare the treatment effect of 3 day miconazole (400 mg/d), 6 day miconazole (400 mg/d), and 7 day miconazole (200 mg/d) for uncomplicated and complicated VVC. RESULTS: Among 300 cases of VVC, uncomplicated, complicated and recurrent VVC were 56.0%, 44.0% and 9.7% (29/300) respectively. C. albicans was isolated most frequently 90.3% (271/300), followed by C. glabrata (7.3%), C. tropicalis (1.3%), C. krusei (0.7%), and C. parapsilosis (0.3 %). Mycologic eradication rate of 3 day, 6 day and 7 day miconazole courses for uncomplicated VVC at day 14 was 96.0%, 93.5% and 98.0%, respectively (P > 0.05). Eradication rate of 3 day, 6 day and 7 day miconazole courses for complicated VVC at day 14 was 86.7%, 92.5%, and 86.4%, respectively (P > 0.05). Eradication rate of 3 day, 6 day and 7 day miconazole courses for uncomplicated VVC at day 35 was 93.8%, 95.3%, and 89.8%, respectively (P > 0.05). Eradication rate of 3 day, 6 day and 7 day miconazole courses for complicated VVC at day 35 was 89.7%, 97.3% and 86.8%, respectively (P > 0.05). CONCLUSION: Treatment of VVC should be individualized, and women with complicated VVC achieve superior mycologic eradication by a 6 day miconazole course.
Adult↗
[Landscape change in Kangbao County of Hebei {rovince].
Based on RS image TM5 of 1999 and SPOT5 of 2003, this paper studied the landscape change from 1999 to 2003 of Kangbao County, Hebei Province. Logistic regression was adopted to analyze the driving factors, and Kappa index was used to evaluate the accuracy of the landscape classification result, which was 86.72% for 1999, and 89.76% for 2003. The area of cropland in Kangbao County reduced largely, while that of vegetable field, forestland and artificial grassland increased sharply, among which, vegetable field and artificial grassland had the greatest increasing rate. The landscape fragmentation aggravated in the region. The landscape change was mainly caused by the policy of reducing cultivated land, with the main driving factor slope, and the direct driving factor water-heat condition and soil fertility determined by slope aspect.
China↗
[Landscape pattern change at the upper reaches of Minjiang River and its driving force].
The upper reaches of Minjiang River is an ecological sensitive and vulnerable area in southwest of China. It is of great significance to the ecological pattern safety in China. In this study, we analyzed the landscape pattern change through the interpretation of TM imageries in 1986, 1995 and 2000. The results showed that the matrix landscape in this area was grass landscape. Forest landscape patches were embedded in the grass landscape. The forest landscape area increased from 1986 to 1995 and decreased from 1995 to 2000. However, the number of patches of forest landscape was increasing during all the time. This suggested that the intensity of anthropogenic disturbances including harvesting, forest landscape reclaiming and excessive grazing were persistently increasing from 1986 to 2000. The ecological driving forces of the landscape change in this area were the intensified anthropogenic disturbances as a result of the population boom including the predacious harvesting of forest and excessive grazing. The natural disturbances such as the global climatic change also partly influenced the landscape change in this area.
China↗
[Application of the third generation cementing technique in total hip anthroplasty].
OBJECTIVE: To investigate the effect of the third generation cementing technique in total hip anthroplasty (THA). METHODS: The clinical data of THA performed on 107 hips in 90 patients suffering from different kinds of orthopedic diseases, 54 males and 36 females, with an average age of 55 years and the average follow-up period of 36 months, were analyzed. RESULTS: According to the Harris scoring system, the 90 patients achieved a score of 34 (range 4 - 78) before the operation and achieved a score of 91 (range 64 - 100) after the operation. 97.1% of the patients showed good or excellent results during the short-term follow-up. CONCLUSION: The third generation cementing technique is rather effective in total hip anthroplasty.
Adult↗
Hydroxyl radical formation is greater in striatal core than in penumbra in a rat model of ischemic stroke.
Although hydroxyl radical ((*)OH) formation has been implicated in the pathophysiological changes of ischemic stroke, (*)OH production in the core and penumbra regions is not clear. It is extremely important to distinguish penumbra from ischemic core in focal cerebral ischemia studies, because the penumbra contains viable tissue, which can be salvaged by appropriate treatment. This study evaluated (*)OH production in both core and penumbra regions of ischemic striatum during ischemia and reperfusion. Microdialysis probes were placed in striatal tissue of rats subjected to the middle cerebral artery occlusion model of ischemic stroke. The (*)OH-trapping agent 4-hydroxybenzoic acid (4-HBA) was administered by both i.v. and probe infusion. Dialysate levels of the 4-HBA oxidation products, 3,4-dihydroxybenzoic acid (3,4-DHBA), were determined by HPLC-ECD. After microdialysis probe delivery of 4-HBA, (*)OH production was significantly increased in the striatal core during both ischemia and reperfusion. Penumbra (*)OH production increased only during reperfusion. Alterations of 3,4-DHBA concentration in dialysate following i.v. 4-HBA administration were likely related to alterations in tissue blood flow. The findings were confirmed by a greater oxidation of dihydroethidium in the ischemic core than in the penumbra as determined by fluorescent microscopy. The findings of (*)OH production in ischemic striatum are the opposite of those reported for ischemic cortex and suggest critical regional variations in (*)OH production that may have significant clinical implications in the treatment of ischemic stroke.
Animals↗
[Study on early heart rate variability in patients with severe acute cerebral vascular disease].
OBJECTIVE: To investigate the dynamic changes of heart rate variability (HRV) in acute cerebral vascular disease, in order to make a criterion for evaluating cerebral function, and establish an assessment for risk stratification after acute stroke. METHODS: All 35 patients who suffered from cerebral vascular disease in the distribution of the carotid artery system were studied from 1 to 7 days following the stroke. HRV analysis with 24-hour Holter tapes was performed every day or every other day continuously for 5 days. According to GCS, the patients were divided into two groups. Critical cerebral vascular disease (CCVD): a state of severe cerebral dysfunction with the Glasgow coma scale (GCS) value between 3 and 8. Non-critical cerebral vascular disease (nCCVD): a state of slightly cerebral dysfunction with the GCS value between 9 and 15. RESULTS: Of the 35 patients, 17 were classified to CCVD, 18 to nCCVD. The CCVD patients had significantly decreased HRV, especially the standard deviation of all R-R intervals (SDNN) and total frequency (TF). The 24-hour heart rate variability graph lost its circadian variation showing a low and plain curve during the whole day. There was a significant correlation between the decrease of HRV and bad prognosis. The borderline of HRV for prognosis was: SDNN<or=60 ms, TF<or=1 500 ms(2)/Hz, high frequency (HF)<or=600 ms(2)/Hz, low frequency (LF)<or=1000 ms(2)/Hz. Dynamic observation proved that if the components of HRV reached this standard, it always indicated bad outcome. On the contrary, if those components increased continuously and were higher than those of the above, it was always indicative of good outcome. These changes always advanced GCS. Prognosis had a high correlate with TF, LF, HF and GCS. Logistic regression showed that only TF, HF and GCS were the main factors affecting the prognosis. The accurate rate of death predication was 88.89 percent; the accurate rate of survival was 82.14 percent. It was much better than the traditional GCS. CONCLUSION: HRV decreases significantly in CCVD and loses its circadian variation. Dynamic observation of HRV offers a good marker for monitoring cerebral function and the progress of disease. HRV could be considered as an objective, non-traumatic and sensitive indicator of the cerebral function and prognosis.
Acute Disease↗
Detection and removal of contaminating hydroxylamines from the spin trap DEPMPO, and re-evaluation of its use to indicate nitrone radical cation formation and S(N)1 reactions.
A previous report that the spin trap 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) allows DEPMPO radical cation formation to be detected via the production of a carbon-centred radical adduct (assigned as the cis-hydroxyethyl species, formed by an intramolecular process) is shown to be incorrect. Rather, this and other paramagnetic species arise from the facile oxidation of trace hydroxylamine impurities present in commercial DEPMPO samples. As a result, techniques for the detection and elimination of such hydroxylamine impurities from DEPMPO solutions were developed and are described; these should prove to be of general use in EPR spin trapping experiments.
Cations↗
Clinical analysis of 26 cases of myositis ossificans circumscripta.
From 1981 to 1997, 26 cases of myositis ossificans circumscripta (MOC) were diagnosed and treated in our hospital, 22 of whom, occupying 84.6%, were confirmed by pathological examination. All the clinic data are reported as follows.
Journal Article↗