Biomedical subjects

Michał Bartoszcze

Publications and source records attributed to Michał Bartoszcze.

3 recordsLinked to original sources

PubMed2006

[Yersinia pestis pathogenesis and diagnostics].

Plague is an acute bacterial infection caused by Gram negative organism Yersinia pestis. This bacteria is subdivided into three classical biotypes: Orientalis, Medievalis and Antiqua. Plague is transmitted via flea vectors from rodents to humans and by respiratory droplets from animals to humans or humans to humans. This agent is on the top of the CDC list of "Critical Biological Agents"--category A. It appears to be a good candidate agent for a bioterrorist attack. Type III secretion (TTS) is a mechanism by which Y. pestis communicates with eukaryotic cells by injecting bacterial proteins across cellular membranes into the cytosol of these cells. These bacterial proteins take control of the host cells by hijacking their intracellular machinery. A laboratory diagnostics of plague is based on: staining techniques, culture on media, immunochromatography, hemagglutination, immunofluorescence, ELISA, phage tests and genetical techniques including: PCR, multiplex and nested PCR, real time PCR, VNTR, PFGE, ISBF and Microarray.

Animals↗

PubMed2005

[Application of the multiplex PCR and PCR-RFLP method in the identification of the Bacillus anthracis].

The aim of this study was to apply the multiplex PCR and PCR-RFLP method for the identification of the B. anthracis strains and to distinguish those bacteria from other members of the Bacillus cereus group. The multiplex PCR method enables to detect the virulence factors, i.e. the toxin and the capsule in B. anthracis strains. To do that, the authors have used 5 primer pairs specific for the fragments of lef, cya, pag genes which are present in the pXO1 plasmid and encode the toxin, the cap gene, which is present in the pXO2 plasmid and encodes the capsule, and the Ba813 chromosomal sequence. Among the four B. anthracis strains examined, three contained two plasmids and the Ba813 chromosomal sequence, while the fourth one contained the pXO1 plasmid only, together and the Ba813 chromosomal sequence. Other bacterial species, belonging to the B. cereus group, were also examined: 6 strains of B. cereus, 4 strains of B. thuringiensis and one strain of B. mycoides. The presence of Ba813 chromosomal sequence has been detected in two B. cereus strains. Neither plasmids nor Ba813 chromosomal sequence have been discovered in other B. cereus, B. thuringiensis and B. mycoides strains. The results of the survey indicate that the Ba813 chromosomal sequence does not occur solely in B. anthracis strains. The PCR-RFLP method with the use of SG-749f and SG-749r primers enabled to demonstrate the presence of DNA sequence (SG-749) in B. anthracis, B. cereus, B. thuringiensis and B. mycoides strains. Restriction analysis with enzyme AluI of the SG-749 sequence, has shown the presence of two DNA fragments at the size of about 90 and 660 bp in all B. anthracis strains. The restriction profile obtained was characteristic for B. anthracis strains and it did not occur in other investigated bacterial species belonging to the B. cereus group. It was not observed even in such B. cereus strains in which the presence of Ba813 sequence was discovered and it enabled to differentiate between B. anthracis strains and other closely related species of the B. cereus group.

Bacillus anthracis↗

PubMed2003

[Methods of biological weapon threats detection].

Detection and identification of biological weapon agents are ones of more important elements of defence against biological weapon and bio-terrorism. So, the attack agent determination creates the chance of necessary prophylactic--medical and liquidation activities undertaking on time. Different systems of biological hazards detection are known. Among others, they allow to detect the biological agents presence in the air, execute the aerosol particles analysis, and are able to generate the alarm signal (LRBSDS, JBSDS, FLAPS). Some systems are able to detect biological agents presence, and moreover, to identify them (JPS, JBAIDS). Luminometric method possesses many advantages, from which the versatility of its usage at different attack scenarios, is getting to stay the most important one. A lot of attention is paid to identification problems, recently. Simple technologies, adjusted to field conditions, which give initial identification result within several minutes (immuno-chromatographic tests), have been developed. These tests, based on colloidal gold may be replaced with UCP particles, what influences positively for identification sensibility and specificity. Systems allowing to execute genetic tests in field conditions (RAPID) and biosensors, thank to which bacteria and their toxins, and viruses can be identified (RAPTOR) have occurred. Huge diagnostic possibilities are created by flow cytometry. Instrumental techniques, among which the mass spectrometry pays attention, are developed. Huge hope is connected to systems working on "chips", allowing, among others, to execute significant amount of analyses simultaneously. Thank to huge expenditures, paid for biological agents identification methods development, part of them, after positive laboratory and field practical tests, shall be introduced into the microbiological practice, staying the valuable contribution of fight against bio-terrorism.

Biological Warfare↗