Biomedical subjects

Michael R Callahan

Publications and source records attributed to Michael R Callahan.

5 recordsLinked to original sources

PubMed2004

In-situ measurements of Cu in an estuarine environment using a portable spectrophotometric analysis system.

Application of a portable in-situ spectrophotometric analysis system for the measurement of Cu in estuarine environments is described in this work. Our spectrophotometric elemental analysis system (SEAS) used for in-situ observations of Cu concentrations is capable of fully autonomous or user-controlled operations. The optical cells used in SEAS systems are flexible liquid core waveguides (LCWs) with optical path lengths as long as 5 m. The 1-m waveguide used in the present study provided a 3.0 nM detection limit and a 5.0% relative standard deviation for a 25 nM copper sample. Analysis times range between 1 and 5 min, allowing for acquisition of data on scales appropriate to the highly dynamic biogeochemical nature of copper in the coastal environment. Field deployments of SEAS-Cu in Tampa Bay, FL, showed low Cu concentrations near the mouth of the estuary (3-4 nM), with elevated concentrations (approximately 25 nM) in anthropogenically impacted regions of the bay (e.g., marinas and areas adjacent wastewater treatment plants). Transect data between Tampa Bay and a deep water harborage exhibited copper concentrations ranging between 5 and 50 nM.

Copper↗

PubMed2003

Use of multiwavelength transmission spectroscopy for the characterization of Cryptosporidium parvum oocysts: quantitative interpretation.

Combined scattering and absorption properties of suspended particles can be obtained as a function of wavelength by measuring the complete ultraviolet-visible (UV-vis) spectrum. This research reports on the quantitative interpretation of measured UV-vis spectra of Cryptosporidium parvum oocyst suspensions obtained from several commercial sources and evaluated using two different purification techniques. The reproducibility of the measured spectral data was assessed, and the quantitative interpretation of the oocyst spectra in terms of the particle size and the chemical composition of the particles are reported herein. The interpretation model of the spectra is based on light scattering theory, spectral deconvolution techniques, and on the approximation of the wavelength-dependent optical properties of the basic constituents of living organisms. A characteristic set of optical properties for C. parvum oocysts has been determined as a function of wavelength and used for the quantitative interpretation of UV-vis spectra. The results from the spectral deconvolution show quantitative differences among oocyst preparations. These results represent the first step in establishing a set of critical parameters (e.g., oocyst size and chemical composition) necessary for the detection and identification of C. parvum oocysts in water using spectroscopy.

Animals↗

PubMed2003

Children's detection of pure-tone signals: informational masking with contralateral maskers.

When normal-hearing adults and children are required to detect a 1000-Hz tone in a random-frequency multitone masker, masking is often observed in excess of that predicted by traditional auditory filter models. The excess masking is called informational masking. Though individual differences in the effect are large, the amount of informational masking is typically much greater in young children than in adults [Oh et al., J. Acoust. Soc. Am. 109, 2888-2895 (2001)]. One factor that reduces informational masking in adults is spatial separation of the target tone and masker. The present study was undertaken to determine whether or not a similar effect of spatial separation is observed in children. An extreme case of spatial separation was used in which the target tone was presented to one ear and the random multitone masker to the other ear. This condition resulted in nearly complete elimination of masking in adults. In young children, however, presenting the masker to the nontarget ear typically produced only a slight decrease in overall masking and no change in informational masking. The results for children are interpreted in terms of a model that gives equal weight to the auditory filter outputs from each ear.

Adolescent↗

PubMed2003

Psychometric functions for informational masking.

The term informational masking has traditionally been used to refer to elevations in signal threshold resulting from masker uncertainty. In the present study, the method of constant stimuli was used to obtain complete psychometric functions (PFs) from 44 normal-hearing listeners in conditions known to produce varying amounts of informational masking. The listener's task was to detect a pure-tone signal in the presence of a broadband noise masker (low masker uncertainty) and in the presence of multitone maskers with frequencies and amplitudes that varied at random from one presentation to the next (high masker uncertainty). Relative to the broadband noise condition, significant reductions were observed in both the slope and the upper asymptote of the PF for multitone maskers producing large amounts of informational masking. Slope was affected more for some listeners and conditions while asymptote was affected more for others; consequently, neither parameter alone was highly predictive of individual thresholds or the amount of informational masking. Mean slopes and asymptotes varied nonmonotonically with the number of masker components in a manner similar to mean thresholds, particularly when the estimated effect of energetic masking on thresholds was subtracted out. As in past studies, the threshold data were well described by a model in which trial-by-trial judgments are based on a weighted sum of levels in dB at the output of independent auditory filters. The psychometric data, however, complicated the model's interpretation in two ways: First, they suggested that, depending on the listener and condition, the weights can either reflect a fixed influence of masker components on each trial or the effect of occasionally mistaking a masker component for the signal from trial to trial. Second, they indicated that in either case the variance of the underlying decision variable as estimated from PF slope is not by itself great enough to account for the observed changes in informational masking.

Acoustic Stimulation↗

PubMed2003

One factor underlies individual differences in auditory informational masking within and across age groups.

Masked threshold for a pure-tone signal can be substantially elevated whenever the listener is uncertain about the spectral or temporal properties of the masker, an effect referred to as auditory informational masking. Individual differences in the effect are large, with young children being most susceptible. When masker uncertainty is introduced by randomizing the frequencies of a multitone masker on each presentation, the function relating a child's pure-tone signal threshold to the number of masker components is found to be substantially elevated above that of most adults. The age effect and the individual differences among adults are not well understood, though a difference in the shapes of the masking functions suggests that different detection strategies may be involved. The present study reports results from a principal components analysis of informational masking functions obtained from 38 normal-hearing children ranging in age from 4 to 16 years and 46 normal-hearing adults ranging in age from 19 to 38 years. The premise underlying the analysis is that if different detection strategies are involved, they should add independent sources of variance to the masking functions. Hence, more than one principal component (PC) should be required to account for a substantial proportion of the variance in these functions. The results, instead, supported the operation of a single underlying strategy with all but 17% of the variance accounted for by the first PC within and across age groups. An analysis of variance on the first two PCs showed that only the first changed with age, and a cluster analysis of the masking functions showed complete separation of clusters along this PC for all but 1 listener. The results are taken to suggest that large individual differences informational masking at all ages reflect differences in the extent to which masker uncertainty adds variance to the decision variable of an otherwise optimal decision strategy.

Adolescent↗