[Factitious and exogenous thyrotoxicosis].
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Anti-Obesity Agents↗
Biomedical subjects
Minehiro Gotoh
Publications and source records attributed to Minehiro Gotoh.
Hypothalamic monoamine metabolism is different between the diabetic GK (Goto-Kakizaki) rats and streptozotocin-induced diabetic rats.
Hypothalamic neuronal activities of noradrenaline, dopamine and serotonin were investigated in the GK (Goto-Kakizaki) rats, a model for type 2 diabetes, and the streptozotocin (STZ)-induced diabetic rats, a model for type 1 diabetes. Each neuronal activity was assessed from a ratio of the concentrations of the major metabolite to those of its precursor monoamine itself or the metabolite concentrations itself. In the GK rats, hypothalamic noradrenaline and dopamine activities were significantly increased, and serotonin activity was unchanged. In the STZ-induced diabetic rats, hypothalamic dopamine and serotonin activities were significantly decreased, and noradrenaline activity was unchanged. In these two diabetic rats, hypothalamic noradrenaline and dopamine activities correlated significantly with serum insulin concentrations, whereas hypothalamic serotonin activity correlated significantly with serum glucose concentrations. These findings suggest that hypothalamic monoamine metabolism of the GK rats is quite different from that of the STZ-induced diabetic rats, and that not only hyperglycemia but also serum insulin concentrations have an influence on the hypothalamic monoamine metabolism in these diabetic rats.
Animals↗
Serum uric acid concentrations in type 2 diabetes: its significant relationship to serum 1,5-anhydroglucitol concentrations.
OBJECTIVE: Serum uric acid concentrations in diabetics are well known to be significantly lower than those in non-diabetic subjects, due to increased its urinary clearance. Serum 1,5-anhydroglucitol concentrations are also specifically decreased in diabetics through the increased urinary excretion. To gain an insight into the idea that a common mechanism might be possible to work in reducing these serum substances, this study was conducted. METHODS: A total of 121 type 2 diabetic patients, 76 males and 45 females, were studied. Multiple regression analysis was performed to determine the independent association between potential predictor variables (mean blood pressure, body mass index, fasting plasma glucose, glycosylated hemoglobin A1c, serum fructosamine, serum 1,5-anhydroglucitol, serum creatinine, serum total cholesterol, and serum triglycerides) and serum uric acid concentrations as the dependent variable. RESULTS: In the male subjects, serum 1,5-anhydroglucitol, serum creatinine and body mass index were the variables independently related to serum uric acid concentrations. In the female subjects, serum 1,5-anhydroglucitol and serum creatinine were the variables independently related to serum uric acid concentrations. CONCLUSION: Considering the glucosuria-related urinary excretion of 1,5-anhydroglucitol, a close positive association between serum uric acid and 1,5-anhydroglucitol concentrations strongly supports the idea that the reduction in serum uric acid concentrations is mediated by urinary glucose excretion in diabetics.
Adult↗
Distribution of thyrostimulin in the rat: an immunohistochemical study.
OBJECTIVE: To identify the distribution of thyrostimulin, a heterodimer of glycoprotein hormone subunits (A2 and B5) by immunohistochemistry in the rat tissues using specific antipeptide anti-serum which we recently produced. METHOD: Anti-thyrostimulin antibody was raised in New Zealand white rabbits immunized with a conjugate of synthetic A2 or B5 with bovine serum albumin. Immunohistochemical analysis was performed by avidin-biotin complex method. RESULTS: Thyrostimulin immunoreactivity was visualized in the anterior pituitary, central nervous system, adrenal gland, stomach, duodenum, pancreas and testis. When using antiserum pre-incubated with synthetic peptides or rat pituitary homogenate which contains thyrostimulin peptide, no significant stain of the pituitary was detected. CONCLUSION: These findings suggest that thyrostimulin is widely distributed and that the method used is valuable in studying the distribution of thyrostimulin in rats.
Animals↗
Radioimmunoassay for aquaporin-9.
OBJECTIVE: To develop radioimmunoassay for aquaporin-9 (AQP9) and search for its presence in certain rat tissues. METHODS: Anti-AQP9 antiserum has been raised in New Zealand white rabbits immunized with a conjugate of synthetic AQP9 with bovine serum albumin. Radioiodination of AQP9 was performed by chloramin T method followed by purification of radioiodinated material on Sephadex G-25 column. RESULTS: The obtained antibody did not crossreact with other aquaporins, hypothalamic hormones, pituitary hormones, neuropeptides or gut hormones. The assay was performed with a double antibody system. AQP9 was extracted from the tissues with acid acetone. The dilution curve of acid acetone extracts of rat liver in the radioimmunoassay system was parallel to the standard curve. The recovery of tissue AQP9 was about 90%, and the intra-assay and inter-assay variations were 4.8% and 7.9%, respectively. AQP9 was found in the liver, testis and brain. CONCLUSION: These data suggest that this assay system is suitable for the estimation of AQP9 in the tissues.
Animals↗