Biomedical subjects

Ming-xing Wu

Publications and source records attributed to Ming-xing Wu.

4 recordsLinked to original sources

PubMed2005

[Changes of blood-aqueous barrier after phacoemulsification in patients with previous glaucoma filtering surgery].

OBJECTIVE: To evaluate the effect of trabeculectomy and/or phacoemulsification surgery on blood-aqueous barrier function. METHODS: Phacoemulsification with implantation of intraocular lens was performed on 46 cataract eyes of 40 glaucoma patients following previous trabeculectomy (group 1), and 64 cataract eyes of 60 patients (group 2). The aqueous flare and cells were examined preoperatively and on days 1, 7, 30, 90 postoperatively using a laser flare-cell meter (LFCM). RESULTS: The mean aqueous flare values were (15.12 +/- 2.87), (40.24 +/- 3.75), (24.33 +/- 3.38), (21.18 +/- 1.77), (16.51 +/- 1.70) photon counts/ms in group 1 preoperatively and on days 1, 7, 30, 90 after surgery (P < 0.05) respectively; and were (6.94 +/- 2.34), (26.27 +/- 10.21), (13.96 +/- 6.44), (9.07 +/- 2.67), (7.16 +/- 1.89) photon counts/ms in group 2 (P < 0.05) respectively. CONCLUSIONS: Trabeculectomy can breakdown blood-aqueous barrier permanently while phacoemulsification damage the blood-aqueous barrier transiently. Phacoemulsification combined with a previous trabeculectomy can enhance this damage.

Aged↗

PubMed2004

[Identification of rabbit lens proteins by two-dimensional gel electrophoreses and mass spectrometry].

OBJECTIVE: To study the role of lens proteomics using two-dimensional electrophoresis (2-DE) and mass spectrometry in order to exploring novel effective ways for cataract prevention and therapy. METHODS: The proteins of the three-month-year old rabbit lens were separated using immobilized pH gradients 2-DE. Image analysis was carried out using Image Master 2D Elite 3.01 software package. Most of the crystallines was identified by matrix assisted laser adsorption/ionization-time of-flight-mass spectrometry (MALDI-TOF-MS). RESULTS: The maps of 2-DE showed that lens proteins were in the section of pH 5 - 9 and the relative molecular weight was 14,000 - 94,000, while relative molecular weight of more abundant crystalline was localized at 14,000 - 40,000. About 180 protein spots were detected with the similar PI, molecular weight and quantity of each spot could be acquired by image analysis software. Sixteen crystallines were identified using MALDI-TOF-MS. CONCLUSION: Proteomic analysis of lens can be accomplished and the proteins can be well separated and analyzed using 2-DE and mass spectrometry. This technique offers a new avenue for analyses of lens proteins and to assess their differential expression in cataract, and may thus provide a novel approach to cataract prevention and therapy.

Animals↗

PubMed2004

[A preliminary report on bimanual microphacoemulsification].

OBJECTIVE: To evaluate the surgical technique, feasibility, and outcome of bimanual microphacoemulsification. METHODS: The preliminary clinical study included 132 senile cataract eyes. A temporal clear cornea incision was made using 19G microvitreoretinal blade with the exterior incision length of 1.4 mm, the interior incision length of 1.2 mm, and the tunnel length of 1.0 mm. A 1.2 mm x 1.0 mm clear cornea side port was created with 19G microvitreoretinal blade at 12 o'clock in the right eye or 6 o'clock in the left eye. A sleeveless titanium phaco needle with an outer diameter of 0.9 mm was inserted through the temporal clear cornea incision. An irrigating chopper was inserted through the side port as the left-hand instrument, bimanual nucleofractis and nuclear emulsification were performed using quick chop technique. The lens cortical removal was performed bimanually with the Duet Bimanual I/A System. The study parameters included phacoemulsification time, intraoperative complications and early postoperative outcome. RESULTS: The mean Phacoemulsification time was 0.75 +/- 0.64 min. vision acuity equal or better than 0.5 were 55.30%, 87.12% and 90.15% after surgery one day, one week and one mother respectively. Eyes with 0.5 best corrected visual acuity amounted to 90.91%, while with 0.8, amounted to 77.27% all the treated eyes 1 month postoperation. The formation of anterior chamber was successfully maintained in every case. There was various degree of nuclear hardness in all of case studied. CONCLUSION: Bimanual microphacoemulsification is a feasible, secure, and effective surgery for cataract extraction through a sub-1.5 mm clear cornea incision.

Adult↗

PubMed2003

[Effects of exogenous p21 gene on cells cycle of human lens epithelial cells].

OBJECTIVE: To investigate the effects of transfected exogenous p21 gene on the cells cycle of HLE-B3 cells line. The feasibility of prevention of secondary cataract by gene therapy was evaluated. METHODS: Total length of human p21 gene cDNA was cloned on the parent's plasmid pcDNA3 to construct the recombinant plasmids of pcDNA3/p21, a large amount of pcDNA3/p21 plasmid DNA was prepared by QIAGEN endofree maxi kit. After harvest of the plasmid DNA, the HLE-B3 cells line was transfected. The cell growth was observed and the cells cycle was analyzed by flow cytometry. The expression of p21 mRNA was detected by RT-PCR and the expression of p21 protein was detected by immunohistochemistry and western blot analysis. RESULTS: Forty eight hours after transfection, the growth of transfected cells became slower, some cells floated and died; the control cells and blank plasmid (blank pcDNA3) transfected cells grew normally. Flow cytometry analysis revealed that the number of cells in G(1) phase increased markedly in transfected cells. The RT-PCR showed that the product of p21 in the transfected cells (dead or alive cells) was obviously higher than that of the controls. Immunohistochemical studies showed few positive cells in the controls, and very high positive signal was detected after transfection. Western blot showed a positive band at the level of 21 000 in transfected cells, no positive band could be found in the controls. CONCLUSION: Exogenous p21 gene can transfect the HLE-B3 cells and can be expressed. The transfection can affect the cells cycle by G(1) arrest and induces cells death through the apoptosis process. This result suggests that it is possible to prevent the occurrence of secondary cataract by gene therapy.

Cell Cycle↗