Biomedical subjects

Mingde Fu

Publications and source records attributed to Mingde Fu.

10 recordsLinked to original sources

PubMed2006

Alterations of high-density lipoprotein subclasses in hypercholesterolemia and combined hyperlipidemia.

BACKGROUND: Alterations in plasma lipid levels can influence the composition, content, and distribution of plasma lipoprotein subclasses that effect atherosclerosis risk. Hypercholesterolemia and combined hyperlipidemia are common forms of atherogenic dyslipoproteinemia. This study evaluates the alterations of high-density lipoprotein (HDL) subclasses in hypercholesterolemic and combined hyperlipidemic subjects. METHODS: Apolipoprotein A-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis in 242 normolipidemic subjects, 66 hypercholesterolemic subjects and 59 combined hyperlipidemic subjects. RESULTS: Compared with the normolipidemic subjects, apolipoprotein A-I contents of small-sized pre-beta1-HDL, HDL3c, HDL3b and HDL3a were significantly higher in both hypercholesterolemic subjects (p<.01, p<.05, p<.01 and p<.05, respectively) and combined hyperlipidemic subjects (p<.01, p<.05, p<.01 and p<.01, respectively). In contrast, apolipoprotein A-I contents of large-sized HDL2a and HDL2b were significantly lower in hypercholesterolemic subjects (p<.05 and p<.01, respectively) as well as combined hyperlipidemic subjects (p<.01 and p<.01, respectively). In addition, pre-beta1-HDL increased significantly (p<.05) while HDL2a and HDL2b decreased significantly (p<.05 and p<.01, respectively) in combined hyperlipidemic group versus hypercholesterolemic subjects. With the elevation of triglyceride levels, pre-beta1-HDL, and HDL3a increased successively, however, HDL2a and HDL2b decreased successively in subjects with total cholesterol levels greater than 240 mg/dl. CONCLUSIONS: The particle size of HDL shifted towards smaller size in hypercholesterolemic subjects, and that the shift was more prominent in combined hyperlipidemic subjects. The alternations mentioned above indicate that HDL maturation might be abnormal, and reverse cholesterol transport (RCT) might be weakened.

Adult↗

PubMed2006

Relationship between total cholesterol/high-density lipoprotein cholesterol ratio, triglyceride/high-density lipoprotein cholesterol ratio, and high-density lipoprotein subclasses.

Alterations in plasma lipid levels can influence the composition, content, and distribution of plasma lipoprotein subclasses that affect atherosclerosis risk. This study evaluated the relationship between plasma total cholesterol (TC)/high-density lipoprotein cholesterol (HDL-C) ratio, triglyceride (TG)/HDL-C ratio, and HDL subclass distribution. The apolipoprotein A-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis coupled with immunodetection in 442 Chinese subjects. The particle size of HDL shifted toward smaller size with the elevation of TC/HDL-C and TG/HDL-C ratios. The ratio of large-sized HDL(2b) to small-sized prebeta(1)-HDL (HDL(2b)/prebeta(1)-HDL) was about 4.7 in the subjects with TC/HDL-C of 3.3 or lower and TG/HDL-C of 2.5 or lower, whereas it was only approximately 1.1 in subjects with TC/HDL-C greater than 6 and TG/HDL-C greater than 5. Pearson correlation analysis revealed that the TC/HDL-C ratio was positively correlated with prebeta(1)-HDL and HDL(3a) but negatively correlated with HDL(2a) and HDL(2b), whereas the TC/HDL-C ratio was only inversely correlated with HDL(2b). The TC/HDL-C and TG/HDL-C ratios together may be a good indicator of HDL subclass distribution. When these 2 ratios increased simultaneously, the trend toward smaller HDL size was obvious, which, in turn, indicated that the maturation of HDL might be impeded and the reverse cholesterol transport might be weakened. In addition, the TG/HDL-C ratio might be a more powerful factor to influence the distribution of HDL subclasses.

Adult↗

PubMed2005

Relationship between plasma HDL subclasses distribution and lipoprotein lipase gene HindIII polymorphism in hyperlipidemia.

BACKGROUND: Different high-density lipoprotein (HDL) subclasses have distinct but interrelated metabolic functions. HDL directly influences the atherogenic process, and changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis. Lipoprotein lipase (LPL) is an important enzyme for hydrolysis of triglyceride-rich lipoproteins, and its activity is positively correlated with the plasma HDL cholesterol level. LPL gene HindIII polymorphism has been found associated with variations in lipid levels, but the impact on HDL subclasses distribution is less clearly established. METHODS: The relative apolipoprotein (apo) A-I contents (% apoA-I) of plasma HDL subclasses were determined by two-dimensional gel electrophoresis coupled with immunodetection and LPL gene HindIII polymorphism was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 173 hyperlipidemic and 155 normolipidemic subjects. RESULTS: The frequencies of 495TT genotype and allele T were the highest both in the hyperlipidemic and control groups. Compared with the control group, the frequency of 495TT genotype was higher, while the frequencies of 495TG and 495GG genotypes were significantly lower (P<0.05) in the hyperlipidemic group. Two-dimensional gel electrophoresis and immunodetection showed that HDL subclasses distribution was altered in hyperlipidemia, and had a general shift toward smaller size. Compared with the control group, the hyperlipidemic group had significantly higher relative apoA-I contents of prebeta1-HDL, prebeta2-HDL, HDL3b and HDL3a (P<0.05) and lower HDL2a and HDL2b levels (P<0.001). In the hyperlipidemic group, allele T carriers' frequency was higher than that in the control group (P<0.05), and the genotype of 495TT showed higher levels of plasma TG, apoB100, TG/HDL-C ratio, relative apoA-I contents of prebeta1-HDL, HDL3b and lower HDL2a, HDL2b compared with that of the 495GG genotype subgroup (P<0.05). In the control group, the genotype of 495TT had higher plasma TG, HDL3c and lower HDL2a compared with that of 495GG subgroup (P<0.05). CONCLUSIONS: The 495TT genotype of LPL gene HindIII polymorphism was associated with changes of HDL subclasses distribution in Chinese population with hyperlipidemia. The particle size of HDL shifted toward smaller, which, in turn, indicated that RCT might be weakened and HDL maturation might be abnormal in hyperlipidemic subjects with 495TT genotype.

Adult↗

PubMed2005

Alterations of high-density lipoprotein subclasses in endogenous hypertriglyceridemia.

BACKGROUND: To investigate the alterations of high-density lipoprotein (HDL) subclasses in endogenous hypertriglyceridemic subjects. METHODS: Apolipoprotein A-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis in 236 normolipidemic subjects (including 146 males and 90 females) and 176 endogenous hypertriglyceridemic subjects (including 103 males and 73 females). RESULTS: Apolipoprotein A-I contents of small-sized pre-beta1-HDL and HDL3a were significantly higher (P < .01 and P < .01, respectively), but those of large-sized HDL2a and HDL2b were significantly lower (P < .01 and P < .01, respectively) in hypertriglyceridemic subjects versus normolipidemic subjects. Moreover, with the elevation of triglyceride levels, small-sized pre-beta1-HDL and HDL3a increased successively; however, large-sized HDL2a and HDL2b decreased successively. Males had significantly higher apolipoprotein A-I contents of small-sized pre-beta1-HDL and HDL3b (P < .05 and P < .05, respectively), but lower contents of large-sized HDL2b (P < .01) than females in both normolipidemic and hypertriglyceridemic subjects. CONCLUSIONS: The particle size of HDL shifted toward smaller size in hypertriglyceridemic subjects, especially in male subjects. Of note, the shift was more obvious with the elevation of triglyceride levels. The changes mentioned above indicate that HDL maturation might be abnormal and reverse cholesterol transport might be weakened.

Female↗

PubMed2005

Relationship between plasma HDL subclasses distribution and apoA-I gene polymorphisms.

BACKGROUND: It is generally accepted that apolipoprotein (apo) A-I is the dominant structural apolipoprotein of HDL particles and different HDL subclasses have distinct but interrelated metabolic functions. HDL is known to directly affect the atherogenic process hence changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis. METHODS: The ApoA-I contents (mg/l) of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection and apoA-I genotypes were assayed by PCR-RFLP in 307 Chinese subjects (169 males, 138 females). RESULTS: The G/G and C/C genotypes were the most frequent at -78 bp and +83 bp of apoA-I gene, respectively. There were no significant differences in the frequencies of rare A allele at -78 bp and rare T allele at +83 bp between males and females. Compared with the G/G carriers, G/A and A/A carriers had significantly higher plasma concentrations of TG, apoC-II, apoC-III, apoA-I contents of prebeta(1)-HDL, HDL(3a) and TG/HDL-C ratio. And in addition, A/A carriers had significantly lower apoA-I contents of HDL(2a) and HDL(2b). Females had increased plasma concentrations of apoA-I, HDL-C, apoA-I contents of HDL(2a) and HDL(2b) while decreased apoA-I contents of prebeta(1)-HDL, HDL(3b) and TG/HDL-C ratio as compared to males carrying the same genotype. No significant differences were demonstrated on the concentrations of plasma lipids, lipoproteins, apolipoproteins and apoA-I contents of plasma HDL subclasses between the C/C and C/T subjects. CONCLUSION: The G/A polymorphism at -78 bp of apoA-I gene was associated with changes of HDL subclasses distribution. There was a general shift towards smaller-sized HDL, which, in turn, indicated that reverse cholesterol transport (RCT) might be weakened and HDL maturation might be abnormal in the subjects with G/A mutation.

Adult↗

PubMed2004

Relationship between plasma lipid concentrations and HDL subclasses.

BACKGROUND: It is generally accepted that different high-density lipoprotein (HDL) subclasses have distinct but interrelated metabolic functions. HDL is known to directly influence the atherogenic process and changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis. METHODS: Apo-AI contents(mg/l) of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection for apo-AI. Four hundred forty-two Chinese adults subjects aged 33 to 78 years were assigned to different groups according to the third Report of NCEP (ATP III) guidelines. The subjects were first divided into 2 groups, normal and high TG, then further classified by plasma TC, HDL-C and LDL-C concentrations. The subjects were also divided into TC desirable and TC high groups. RESULTS: Apo-A contents of prebeta(1)-HDL were higher while HDL(2b) were lower in high TG subjects vs. the corresponding normal TG subjects according to plasma TC and LDL-C concentrations. With the increase of plasma TC concentrations, apo-AI contents of prebeta(1)-HDL were significantly higher in high TC subgroup vs. TC desirable subgroup in normal TG subjects. With the decrease of HDL-C concentrations, apo-AI contents of HDL(2b) tended to decrease in normal TG subjects. And, with the increases of LDL-C concentration, in normal TG subjects, apo-AI contents of prebeta(1)-HDL and HDL(3b) were significantly higher and those of HDL(2b) were significantly lower in very high LDL-C subgroup vs. LDL-C optimal subgroup. On the other hand, apo-AI contents of prebeta(1)-HDL and HDL(3a) were significantly higher, while HDL(2a) and HDL(2b) were significantly lower in high TG and very high TG subgroup vs. normal TG subgroup within either TC desirable or TC high subjects. In a multivariate linear regression model, TG and TC concentrations were all associated independently and positively with high prebeta(1)-HDL; however, HDL-C were inversely associated with high prebeta(1)-HDL. And TG and TC concentrations were all associated independently and negatively with low HDL(2b), but HDL-C and apo-AI were positively associated with low HDL(2b). CONCLUSIONS: With the increase of plasma TG, TC, LDL-C or the decrease of plasma HDL-C concentrations, there was a general shift toward smaller-sized HDL, which, in turn, indicates that reverse cholesterol transport might be weakened and HDL maturation might be abnormal. Plasma TG concentration is a more important factor than TC concentration on the changes of HDL subclass distribution. Moreover, when TG is normal and HDL-C decreased, large-size HDL particles tended to decrease.

Adult↗

PubMed2003

Alterations of HDL subclasses in hyperlipidemia.

BACKGROUND: It is generally accepted that different high-density lipoprotein (HDL) subclasses have distinct but interrelated metabolic functions. HDL is known to directly influence the atherogenic process and changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis. METHOD: The relative apolipoprotein (apo)A-I contents (% apoA-I) of plasma HDL subclasses were determined by two-dimensional gel electrophoresis coupled with immunodetection for apoA-I, in 39 hypercholesterolemic (HTC) subjects, 97 hypertriglyceridemic (HTG) subjects and 32 mixed hyperlipidemic (MHL) subjects, and 124 normolipidemic subjects. RESULTS: The relative apoA-I contents of prebeta(1)-HDL, prebeta(2)-HDL, HDL(3c), HDL(3b) and HDL(3a) significantly increased while HDL(2a) and HDL(2b) significantly decreased in hyperlipidemic subjects. In HTC subjects of hyperlipidemia, the concentrations of prebeta(1)-HDL were significantly lower and HDL(2b) concentrations were significantly higher than in HTG and MHL subjects. In HTG subjects, the concentrations of HDL(3a) were significantly higher and the concentrations of HDL(2b) were lower than in HTC and MHL subjects. In total hyperlipidemic subjects, plasma triglyceride (TG) concentrations showed positive correlation with prebeta(1)-HDL, prebeta(2)-HDL, HDL(3b) and HDL(3a) and negative correlation with HDL(2a) and HDL(2b). The total cholesterol (TC) concentrations showed positive correlation with the relative apoA-I contents of prebeta(1)-HDL and HDL(3b), whereas the HDL-C concentrations showed negative correlation with the relative apoA-I contents of prebeta(1)-HDL and HDL(3a) and positive correlation with those of HDL(2a) and HDL(2b). The relative apoA-I contents of prebeta(1)-HDL, prebeta(2)-HDL, HDL(3b), and HDL(3a) were positively correlated whereas those of HDL(2a) and HDL(2b) were negatively correlated with TG/HDL-C ratio. CONCLUSION: The particle size of HDL in hyperlipidemic subjects shifted towards smaller sizes, which, in turn, indicates that the maturation of HDL may be abnormal in hyperlipidemic subjects.

Adult↗

PubMed2003

[Subclasses of serum HDL in hyperlipidemia].

OBJECTIVE: To detect the change of composition and ratio of serum HDL subclasses and explore the relationship between these changes and the plasma lipid level in patients with hyperlipidemia. METHODS: The subclasses of serum HDL in 168 patients with hyperlipidemia were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method. RESULTS: Compared to the healthy controls, the pre-beta 1 HDL, pre-beta 2 HDL, HDL3c, HDL3b, and HDL3a increased significantly (P < 0.01, P < 0.01, P < 0.05, P < 0.01 and P < 0.01), while the HDL2a and HDL2b decreased significantly (P < 0.01) in the patients with hyperlipidemia. The pre-beta 1 HDL and HDL3b were significantly higher (P < 0.01) while the HDL2a and HDL2b were significantly lower (P < 0.01) in men than in women with hyperlipidemia. The pre-beta 1 HDL was significantly higher (P < 0.05) while the HDL2b was significantly lower (P < 0.05) in the male control group than in the female control group. In the patients with hyperlipidemia, the serum TG was positively correlated with pre-beta 1 HDL, pre-beta 2 HDL, HDL3b, and HDL3a (r = 0.583, 0.196, 0.130, and 0.370 respectively) and negatively correlated with HDL2a and HDL2b (r = -0.293 and -0.456 respectively); The serum TC was positively correlated with pre-beta 1 HDL and HDL3b (r = 0.348 and 0.219); The serum LDL-C was positively correlated with HDL3b (r = 0.187); The serum HDL-C was negatively correlated with pre-beta 1 HDL and HDL3a (r = -0.354 and -0.292 respectively) and positively correlated with HDL2a and HDL2b (r = 0.254 and 0.256); The TG/HDL-C ratio was positively correlated with pre-beta 1 HDL, pre-beta 2 HDL, HDL3b, and HDL3a (r = 0.593, 0.225, 0.123, and 0.394) and negatively correlated with HDL2a and HDL2b (r = -0.317 and -0.459). CONCLUSION: The particle of HDL in the patients with hyperlipidemia shows a general shift towards smaller size, which indicates that the maturation of HDL could be abnormal in patients with hyperlipidemia. The change of subclasses of HDL in the patients with hyperlipidemia might be associated with coronary artery diseases.

Adult↗

PubMed2003

[Study on the total activity of PKC and the quantity of PKC (alpha, beta) in left auricle tissues of the patients with mitral disease and atrial fibrillation].

OBJECTIVE: To investigate the possible relation of atrial fibrillation (AF) with the total activity of protein kinase C (PKC) and the quantity of PKC (alpha, beta) in the left auricle tissues of the patients with mitral disease. METHODS: Thirty-five patients hospitalized for valve replacement surgery were divided into four groups: group A (simple mitral stenosis with AF), group B(simple mitral stenosis with sinus rhythm), group C(simple mitral regurgitation with AF), group D (simple mitral regurgitation with sinus rhythm), The total PKC, activity and the quantity of PKC alpha and PKC beta were measured and compared in an attempt to find out whether significant difference exists between the groups. RESULTS: There was no significant difference in total PKC activity between the four groups (P > 0.05). The quantity of PKC alpha in patients with AF was lower than that in patients with sinus rhythm, the quantity of PKC beta in patients with AF was higher than in patients with sinus rhythm. But these differences were not statistically significant (P > 0.05). CONCLUSION: Under conditions of the same rhythm and the same heart disease, the total PKC activity and the quantity of PKC (alpha, beta) of the four groups showed no significant difference. No obvious relation was seen between the total activity of PKC in left auricle tissue and the occurrence of AF in patients with mitral disease, but more research on the relation between AF and the quantity of PKC isoforms in left auricle tissues would be needed.

Atrial Fibrillation↗

PubMed2003

[Subclasses of serum HDL in 151 healthy adults in Chengdu].

OBJECTIVE: To investigate the components of subclasses of serum HDL in healthy adults and the impact of age and gender on these components. METHODS: The components of subclasses of serum HDL in 151 healthy adults were determined by two dimensional gel electrophoresis associated with immunodetection method. RESULTS: The average concentration of prebeta1-HDL, prebeta2-HDL, HDL(3c), HDL(3b), HDL(3a), HDL2a, and HDL(2b) were 6.11%, 4.74%, 5.97%, 11.70%, 20.27%, 21. 85%, and 29.19%, respectively. The prebeta1-HDL increased with age, whereas the HDL(2b) decreased. The components of prebeta1-HDL in men over 50 and women over 60 were significantly greater than those of the same gender who were younger than 40, while the components of HDL2b in both men and women over 50 decreased compared to those of the same gender who were younger than 40. The component of prebeta1-HDL was significantly greater in men than in women, but it was reversed for HDL(2b). The prebeta1-HDL increased with the body mass index, age, and the concentration of TG. The HDL2b decreased with age and the concentrations of TG and apoB100. CONCLUSION: With the increase of age, there is a general shift toward smaller sized HDL, which indicates that the reverse cholesterol transport might be weakened. Men has smaller particle size than women.

Adult↗