Biomedical subjects

Misun Lee

Publications and source records attributed to Misun Lee.

3 recordsLinked to original sources

PubMed2005

Regulation of zymogen granule exocytosis by Ca2+, cAMP, and PKC in pancreatic acinar cells.

The effect of cAMP and PKC on zymogen granule exocytosis was investigated by simultaneously measuring cytosolic Ca2+ concentration ([Ca2+]c) and individual zymogen granule exocytosis in isolated mouse pancreatic acini. When acinar cells were stimulated with acetylcholine (ACh, 10 microM), exocytic events were detected through granule-attached apical membranes with [Ca2+]c rise. Application of secretin, forskolin (an adenylate cyclase activator), or PMA (a PKC activator) alone did not elicit any [Ca2+]c rise or zymogen granule exocytosis, but co-stimulation with ACh led to exocytosis in that the total number of secreted granules increased markedly without a significant difference in [Ca2+]c rises. When we evoked exocytosis by [Ca2+]c ramps, pretreatment with forskolin or PMA elicited exocytosis at lower [Ca2+]c levels. These results indicate that PKC or cAMP alone could not directly elicit zymogen granule exocytosis, but that they increase the total releasable pool by rendering zymogen granules more sensitive to Ca2+.

Animals↗

PubMed2005

A basic helix-loop-helix transcription factor regulates cell elongation and seed germination.

Plants are sessile and rely on a wide variety of growth hormones to adjust growth and development in response to internal and external stimuli. We have identified a gene, designated NAN, encoding a basic helix-loop-helix (bHLH) transcription factor that regulates cell elongation and seed germination in plants. NAN has an HLH motif in its C-terminal region but does not have any other discernible homologies to bHLH proteins. A bipartite nuclear localization signal is located close to the HLH motif. An Arabidopsis mutant, nan-1D, in which NAN is activated by the insertion of the 35S enhancer, exhibits growth retardation with short hypocotyls and curled leaves. It is also characterized by reduced seed germination and apical hook formation, symptomatic of GA deficiency or disrupted GA signaling. The phenotypic effects of nan-1D were increased by treatment with paclobutrazol (PAC), an inhibitor of gibberellic acid (GA) biosynthesis. NAN is constitutively expressed throughout the life cycle. Our observations indicate that NAN has a housekeeping role in plant growth and development, particularly in seed germination and cell elongation, and that it may modulate GA signaling.

Amino Acid Sequence↗

PubMed2004

Morphological and functional changes of dissociated single pancreatic acinar cells: testing the suitability of the single cell as a model for exocytosis and calcium signaling.

Isolated single pancreatic acinar cells have long been used as a model for studying many kinds of signaling processes due to their structural and functional polarities, but without significant validation. In this study, we examined the morphological and functional changes of dissociated single pancreatic acinar cells. Acutely isolated single cells showed a collapsed membrane potential and a much reduced secretion of zymogen granules in response to acetylcholine (ACh) stimulation, whereas clustered cells showed a much more negative membrane potential and potent exocytotic secretion. The isolated single cells became vertically flattened due to the loss of supporting adhesions with nearby cells, and the granule-attached luminal membrane was severely reduced versus that of clustered cells. However, polarized Ca(2+) signals and mitochondrial localizations were relatively well preserved in the isolated single cells, in that Ca(2+) release by ACh commenced at the indented luminal membrane. In clusters, the Ca(2+) release site was closest to the lumen where more than three cells met or at the tips of conical regions of the luminal membrane. These findings suggest that the dissociated single pancreatic acinar cells preserve an intact Ca(2+) signaling machinery but alter in shape and have impaired exocytotic functions and resting membrane potentials.

Acetylcholine↗