Biomedical subjects

Miyako Kusano

Publications and source records attributed to Miyako Kusano.

11 recordsLinked to original sources

PubMed2026

OsICL-associated metabolic reprogramming during dehydration in rice is regulated by ABA and modulated by ACC and its metabolites.

Drought coordinates hormonal, transcriptional, and metabolic reprogramming, but how abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC) jointly shape cereal dehydration responses remains unclear. We integrated hormone profiling, transcriptome and promoter analyses, synthetic promoter assays, and metabolite profiling in rice. ABA and ACC contents increased markedly in rice shoots under moderate soil water deficit. Combined ABA + ACC treatment showed larger absolute overlaps with dehydration-responsive genes than either ABA or ACC treatment alone in shoots; in roots, this pattern was observed for induced but not repressed genes. Promoters of dehydration- and ABA-inducible genes were enriched in ACGT-core motifs, including a CGTACG core preferentially embedded in ACGTACGT, designated the eXtended ACGT box (Xbox). Multimerised Xbox conferred transcriptional induction under soil water deficit and in response to ABA. OsICL was induced under soil water deficit and by ABA or ACC; in shoots, combined ABA + ACC treatment produced the highest mean transcript accumulation. OsICL overexpression and knockout lines showed altered organic-acid, sugar, and amino-acid profiles, particularly under soil water deficit, but several metabolites changed in the same direction in both line classes. These findings support an ABA-centred, ACC-modulated model of dehydration-responsive transcription and associate OsICL regulation with broader, condition-dependent changes in primary metabolism.

Oryza sativa↗

PubMed2006

Predictive metabolite profiling applying hierarchical multivariate curve resolution to GC-MS data--a potential tool for multi-parametric diagnosis.

A method for predictive metabolite profiling based on resolution of GC-MS data followed by multivariate data analysis is presented and applied to three different biofluid data sets (rat urine, aspen leaf extracts, and human blood plasma). Hierarchical multivariate curve resolution (H-MCR) was used to simultaneously resolve the GC-MS data into pure profiles, describing the relative metabolite concentrations between samples, for multivariate analysis. Here, we present an extension of the H-MCR method allowing treatment of independent samples according to processing parameters estimated from a set of training samples. Predictions or inclusion of the new samples, based on their metabolite profiles, into an existing model could then be carried out, which is a requirement for a working application within, e.g., clinical diagnosis. Apart from allowing treatment and prediction of independent samples the proposed method also reduces the time for the curve resolution process since only a subset of representative samples have to be processed while the remaining samples can be treated according to the obtained processing parameters. The time required for resolving the 30 training samples in the rat urine example was approximately 13 h, while the treatment of the 30 test samples according to the training parameters required only approximately 30 s per sample (approximately 15 min in total). In addition, the presented results show that the suggested approach works for describing metabolic changes in different biofluids, indicating that this is a general approach for high-throughput predictive metabolite profiling, which could have important applications in areas such as plant functional genomics, drug toxicity, treatment efficacy and early disease diagnosis.

Animals↗

PubMed2005

Brevicompanine C, cyclo-(D-Ile-L-Trp), and cyclo-(D-Leu-L-Trp), plant growth regulators from Penicillium brevi-compactum.

New plant growth regulators, named brevicompanine C (1), cyclo-(D-Ile-L-Trp) (2), and cyclo-(D-Leu-L-Trp) (3), have been isolated from Penicillium brevi-compactum Dierckx, and their structures have been established by spectroscopic methods including 2D NMR and chiral TLC analysis. Plant growth activities of 1, 2, and 3 have been examined using lettuce seedling bioassay methods. All compounds accelerated the root growth of the seedlings in proportion to their concentration from 1 to 100 mg/L.

Amino Acid Sequence↗

PubMed2005

Stimulation of mycelia growth in several mushroom species by rice husks.

When supplemented to the culture medium of mushroom Coprinus cinereus, rice husks soaked beforehand in methanol stimulated mycelia growth up to a concentration of 80 mg/ml dose-dependently, whereas the non-treated stimulated mycelia growth up to 20 mg/ml. This result suggests the existence of both stimulatory and inhibitory substances in rice husks. Since momilactone A (MLA) is recognized as one of the phytoalexins in rice husks, its biological activity against mycelia growth was tested. Momilactone A inhibited mycelia growth at 5 microg/disc, whereas the methanol extract of husks did so at 1 mg/disc, wherein 0.2 microg of MLA was estimated by LC/MS/MS. Thus the phytoalexins including MLA should inhibit mycelia growth. Rice husks stimulated mycelia growth in some edible mushroom species such as Grifola frondosa (maitake), Lentinus edodes (shiitake), Pleurotus eryngii (eringi), and P. ostreatus (hiratake). Our findings might lead to the development of new profitable cultivation methods for mushrooms using rice husks.

Agaricales↗

PubMed2004

A strategy for identifying differences in large series of metabolomic samples analyzed by GC/MS.

In metabolomics, the purpose is to identify and quantify all the metabolites in a biological system. Combined gas chromatography and mass spectrometry (GC/MS) is one of the most commonly used techniques in metabolomics together with 1H NMR, and it has been shown that more than 300 compounds can be distinguished with GC/MS after deconvolution of overlapping peaks. To avoid having to deconvolute all analyzed samples prior to multivariate analysis of the data, we have developed a strategy for rapid comparison of nonprocessed MS data files. The method includes baseline correction, alignment, time window determinations, alternating regression, PLS-DA, and identification of retention time windows in the chromatograms that explain the differences between the samples. Use of alternating regression also gives interpretable loadings, which retain the information provided by m/z values that vary between the samples in each retention time window. The method has been applied to plant extracts derived from leaves of different developmental stages and plants subjected to small changes in day length. The data show that the new method can detect differences between the samples and that it gives results comparable to those obtained when deconvolution is applied prior to the multivariate analysis. We suggest that this method can be used for rapid comparison of large sets of GC/MS data, thereby applying time-consuming deconvolution only to parts of the chromatograms that contribute to explain the differences between the samples.

Algorithms↗

PubMed2004

Penipratynolene, a novel nematicide from penicillium bilaiae Chalabuda.

New acetylenic nematicidal compound, penipratynolene (1), methy (2'R)-4-(2'-hydroxy-3'-butynoxy)benzoate, together with two known compounds, 6-methoxycarbonylpicolinic acid (2) and 2,6-pyridinedicarboxylic acid (3), were isolated from the culture filtrate of Penicillium bilaiae Chalabuda. The structures of 1-3 were established by spectroscopic methods. The absolute configuration of 1 was confirmed by using a modified version of Mosher's method. Compounds 1-3 showed nematicidal activity of 77%, 52%, and 98%, respectively, by a bioassay at 300 mg/l with the root-lesion nematode Pratylenchus penetrans.

Alkynes↗

PubMed2003

Betagamma-dehydrocurvularin and related compounds as nematicides of Pratylenchus penetrans from the fungus Aspergillus sp.

The new nematicidal compound, betagamma-dehydrocurvularin (1), together with three known compounds, alphabeta-dehydrocurvularin (2), 8-beta-hydroxy-7-oxocurvularin (3) and 7-oxocurvularin (4), were isolated from the culture filtrate and mycelial mats of Aspergillus sp. The structures of 1-4 were established by spectroscopic methods including 2D NMR. The biological activities of 1-4 were examined by bioassays with root-lesion nematodes, and lettuce and rice seedlings.

Animals↗

PubMed2003

Hydroxysulochrin, a tea pollen growth inhibitor from the fungus Aureobasidium sp.

A new plant growth regulator, hydroxysulochrin (1), together with sulochrin (2) was isolated from the culture filtrate of Aureobasidium sp. grown on a malt extract medium. The structures of 1 and 2 were established by spectroscopic methods. 1 and 2 inhibited tea pollen tube growth by 41% and 36% of the control value at a concentration of 100 mg/l, respectively. However, 1 and 2 showed no inhibitory effect on the growth of lettuce seedlings from 0.1 mg/l to 100 mg/l.

Benzoates↗

PubMed2002

Myxostiolide, myxostiol, and clavatoic acid, plant growth regulators from the fungus Myxotrichum stipitatum.

New plant growth regulators, named myxostiolide (1), myxostiol (2), and clavatoic acid (3), have been isolated from Myxotrichum stipitatum, and their structures have been established by spectroscopic methods including 2D NMR. The biological activities of 1, 2, and 3 have been examined using tea pollen and lettuce seedling bioassay methods. With tea pollen, compound 1 inhibited the pollen tube growth to 14% of control at a concentration of 100 mg/L. With lettuce seedlings, compound 2 accelerated the root growth from 1 mg/L to 100 mg/L and compound 3 inhibited the root growth, to 52% of control, at a concentration of 100 mg/L.

Benzoates↗

PubMedDate not supplied

Aspterric acid and 6-hydroxymellein, inhibitors of pollen development in Arabidopsis thaliana, produced by Aspergillus terreus.

Aspterric Acid, 6-Hydroxymellein, Arabidopsis thaliana, Aspergillus terreus Aspterric acid (1) and 6-hydroxymellein (2), inhibitors of pollen development in Arabidopsis thaliana, have been isolated from the fungus Aspergillus terreus. 1 and 2 inhibited the pollen development at concentrations of 38 and 52 microM, respectively. The microscopic examination of pollen development suggested that the inhibition by the treatment with 1 caused at meiosis and the inhibition by the treatment with 2 caused at microspore stage. 1 and 2 could be useful agents for the molecular investigation of anther and pollen development in higher plants.

Arabidopsis↗

PubMedDate not supplied

4-Hydroxykigelin and 6-demethylkigelin, root growth promoters, produced by Aspergillus terreus.

Root growth promoters, 4-hydroxykigelin (1) and 6-demethylkigelin (2), together with 6-hydroxymellein (3) were isolated from cultures of the fungus Aspergillus terreus and their structures were identified by spectroscopic analysis. The biological activities of the three dihydroisocoumarins, 1, 2, and 3, have been examined using a bioassay method with lettuce seedlings. Furthermore, interactions between the dihydroisocoumarins and indole-3-acetic acid against the root growth have been examined.

Aspergillus↗