Biomedical subjects

Mojca Vilfan

Publications and source records attributed to Mojca Vilfan.

5 recordsLinked to original sources

PubMed2006

Interparticle potential and drag coefficient in nematic colloids.

Magneto-optic tweezers were used for measurements of liquid-crystal-mediated forces between spherical beads with tangential anchoring in thin nematic samples. Repulsive force, which results from the quadrupolar symmetry of defects around the immersed beads, decreases proportionally to 1/x6, with x being the bead separation. The velocity with which the particles are pushed apart also follows the same separation dependence. We thus find the effective drag coefficient gamma(eff) independent of x for surface-to-surface distances as small as 10% of the bead diameter.

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PubMed2005

Force-dependent stepping kinetics of myosin-V.

Myosin-V is a processive two-headed actin-based motor protein involved in many intracellular transport processes. A key question for understanding myosin-V function and the communication between its two heads is its behavior under load. Since in vivo myosin-V colocalizes with other much stronger motors like kinesins, its behavior under superstall forces is especially relevant. We used optical tweezers with a long-range force feedback to study myosin-V motion under controlled external forward and backward loads over its full run length. We find the mean step size remains constant at approximately 36 nm over a wide range of forces from 5 pN forward to 1.5 pN backward load. We also find two force-dependent transitions in the chemomechanical cycle. The slower ADP-release is rate limiting at low loads and depends only weakly on force. The faster rate depends more strongly on force. The stronger force dependence suggests this rate represents the diffusive search of the leading head for its binding site. In contrast to kinesin motors, myosin-V's run length is essentially independent of force between 5 pN of forward to 1.5 pN of backward load. At superstall forces of 5 pN, we observe continuous backward stepping of myosin-V, indicating that a force-driven reversal of the power stroke is possible.

Animals↗

PubMed2003

Azimuthal and zenithal anchoring of nematic liquid crystals.

Temperature dependence of azimuthal and zenithal anchoring energy coefficients of the nematic liquid crystal 4-n-pentyl-4(')-cyanobiphenyl on rubbed nylon is measured using dynamic light scattering. The method is based on observations of director fluctuations in a planarly aligned wedge cell, where the anchoring energy coefficients can be obtained without any external torques acting on the liquid crystal during the measurement. We found that both anchoring coefficients decrease steadily on approaching the nematic-isotropic transition. Moreover, in the whole temperature range of the nematic phase, the ratio between the zenithal and the azimuthal anchoring coefficients is almost equal to the ratio between the splay and the twist Frank elastic constants. The same result is obtained also for the nematic phase of 4-n-octyl-4(')-cyanobiphenyl. This indicates that the aligning nylon layer directly affects only the monomolecular layer at the surface whereas the observed anchoring is governed by the elastic properties of the alkyl-cyanobiphenyl.

Journal Article↗

PubMed2002

Deuteron NMR study of molecular ordering in a holographic-polymer-dispersed liquid crystal.

Using deuteron nuclear magnetic resonance (NMR) and dynamic light scattering, we study the orientational order and dynamics of a BL038-5CB liquid-crystal mixture in a holographic polymer dispersed liquid-crystal material (HPDLC) as used for switchable diffractive optical elements. At high temperatures, where the liquid crystal is predominantly in the isotropic phase, the HPDLC deuteron NMR linewidth and transverse spin-relaxation rate T-12 are two orders of magnitude larger than in the bulk. The analysis shows that the surface-induced order parameter in HPDLC is significantly larger than in similar confining systems and that translational diffusion of molecules in the surface layer is at least two orders of magnitude slower than in the rest of the cavity. The unusual temperature dependence of T-12 upon cooling suggests the possibility of a partial separation of the 5CB component in the liquid-crystal mixture. The onset of the nematic phase in HPDLC occurs at considerably lower temperature than in the bulk and takes place gradually due to different sizes and different content of non-liquid-crystalline ingredients in droplets. Parts of the droplets are found isotropic even at room temperature and the structure of the nematic director field in the droplets is only slightly anisotropic. We point out the capability of NMR to detect the actual state of liquid-crystalline order in HPDLCs and to contribute in this way to the improvement of the switching efficiency of diffraction gratings.

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PubMed2002

Dynamic light scattering measurements of azimuthal and zenithal anchoring of nematic liquid crystals.

Dynamic light scattering is used to obtain the anchoring energy coefficients of nematic liquid crystal (4-n-pentyl-4(')-cyanobiphenyl) on rubbed nylon and photoactive poly-(vinyl-cinnamate). The anchoring coefficients are determined by measuring the relaxation time of the fundamental director fluctuation mode in a homogeneously aligned wedge cell as a function of cell thickness. The method is nonperturbative as no external torque is applied to the liquid crystal during the measurement. We show that by using two different scattering geometries, both azimuthal and zenithal anchoring energy coefficients can be measured on the same sample. The obtained zenithal anchoring coefficient is found to be, in contrast to previously reported results, approximately only two times larger than the azimuthal one. The effect of higher order fluctuation modes on the detected autocorrelation function is in good agreement with numerical calculations.

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