Biomedical subjects

Mu Xian

Publications and source records attributed to Mu Xian.

3 recordsLinked to original sources

PubMed2005

[Impact of olfactory nerve transection on the apoptosis of mice olfactory receptor neurons].

OBJECTIVE: To analyze the impact of olfactory nerve transection on the apoptosis of mice olfactory receptor neurons (ORN), and discuss the reliability of this experimental model. METHODS: After olfactory nerve transection of mice, anterograde horseradish peroxidase (HRP) tracing was performed to confirm the completion of nerve transection. On 8 h, 2 d, 3 d and 5 d after surgery, TdT mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL) was used to observe the apoptosis of ORN, while relative semi-quantitative RT-PCR and immunohistochemistry were used to reflect the expression of olfactory marker protein (OMP, special marker of mature ORN) in olfactory epithelium. RESULTS: No HRP label was observed in olfactory bulb after olfactory nerve transaction. Both TUNEL-positive and OMP-positive cells were ORN. After the surgery, TUNEL-positive cells increased remarkably and peaked on 2 d after the surgery. Meanwhile OMP mRNA in olfactory epithelium began to decrease markedly till 5 d after the surgery, and the olfactory epithelium got thinner accordingly. CONCLUSIONS: This experimental model can be used reliably to sever mice olfactory nerve and manipulate simultaneous apoptosis of mice ORN.

Animals↗

PubMed2004

[Electrically stimulated olfactory evoked potential in olfactory-lesioned rabbit].

OBJECTIVE: To develop olfactory dysfunctioned animal model by injuring olfactory blub(s) and to investigate the characteristics of olfactory evoked potential. METHODS: The changes of OEP in rabbits were observed at 24 h, 48 h and 1 w after olfactory bulb(s) were electrolytically injured. The pathological and ultrastructural changes of olfactory mucosa and olfactory bulb were also observed. RESULTS: When unilateral olfactory bulb was damaged, N2 vanished with elongated latencies and lowered amplitudes of N1 and P1. When both olfactory bulbs were damaged, N1 and N2 were absent, only P1 could be recorded. The latency was extended obviously and the amplitude had a big change. Histopathology and ultrastructure showed that there were a lot of inflammatory cells and degenerated neurons scattered around OB 24 hours to 48 hours after injure. It was observed that the olfactory cilia had distraction perversion and colliquefaction changes. CONCLUSION: Different degree of olfactory bulb injury caused different effect on OEPs. These changes of potential were based on the histopathology and ultrastructure.

Animals↗

PubMed2003

[Experimental study on olfactory evoked potential in rabbits].

OBJECTIVE: To observe olfactory evoked potential(OEPs) produced by electrical stimulation of the olfactory mucosa and to find out an objective way to appraise olfactory function. METHOD: A bipolar stimulus electrode was placed on the olfactory mucosa of adult health rabbits via anterior naris. Olfactory evoked potential was recorded at headtop after being given a 0.5-4.0 mA current to the mucosa. And we also investigated the effect of stimulus parameters and stimulus location to the wave form of OEPs. RESULT: Triphasic negative-positive-negative evoked potentials were recorded from the surface of the frontal head close to olfactory bulb. Latent period of three waves respectively was N(1)20.6 ms, P(1)33.5 ms, N(2)58.3 ms. CONCLUSION: In general, the OEPs were composed of triphasic negative-positive-negative complexes. The waveforms were stable and could be repeated well. Each parameter was useful for estimating the olfactory function.

Animals↗