Biomedical subjects

Mutsumi Matsushita

Publications and source records attributed to Mutsumi Matsushita.

6 recordsLinked to original sources

PubMed2004

Consistency of low bone density across bone sites in SAMP6 laboratory mice.

The development of bone densitometry has made it clear that there are discrepancies in bone density at various measurement sites in a given individual. This study examined the consistency of bone density measurements across various sites in a strain of laboratory mouse (senescence-accelerated mouse; SAM). A systemic evaluation of the bone density was performed by dual-energy X-ray absorptiometry (DXA) on SAMP6 (P6) mice, a strain with low peak bone density, as measured by microphotodensitometry of the femoral bones, whereas the SAMP2 (P2) and SAMR1 (R1) strains have high peak bone density. We modified Jilka's method to more comprehensively measure the whole body and additional regions of interest (ROIs; head, right foreleg, left foreleg, right hindleg, left hindleg, spine, and tail). The age-related changes in the total (whole-body) BMD showed a common pattern among the strains studied, and the peak value was seen at 4 months old. P6 showed the lowest peak BMD. A detailed comparison of the bone density between P6 and P2 at the age of 4 months revealed significantly lower regional BMD values for P6 in all seven ROIs. The strain difference in BMD could not be attributed to a difference in size. In conclusion, P6 mice showed low bone density not only in their femurs but also in the subregions and over their entire body. This strain can be potentially useful in the investigation of the genetic basis of senile osteoporosis.

Absorptiometry, Photon↗

PubMed2003

Atlantoaxial transarticular screw fixation for a high-riding vertebral artery.

STUDY DESIGN: The feasibility of inserting a screw for the narrow isthmus with a high-riding vertebral artery was evaluated in patients subjected to posterior atlantoaxial transarticular screw fixation. OBJECTIVE: To demonstrate the feasibility of inserting bilateral screws and obtaining a stable atlantoaxial complex for patients with a high-riding vertebral artery. SUMMARY OF BACKGROUND DATA: Posterior atlantoaxial transarticular screw fixation entails the potential risk of vertebral artery injury, which may be lethal. The risk is much higher for the narrow isthmus caused by a high-riding vertebral artery, and many authors recommend that the procedure should be abandoned if the isthmus is too narrow. On the other hand, bilateral screw fixation is stronger than unilateral screw fixation. METHODS: For this study 27 consecutive patients who submitted to atlantoaxial transarticular screw fixation were evaluated before surgery for the position of the vertebral artery grooves using computed tomography (CT) reconstruction. Seven of the patients were defined as having a unilateral high-riding vertebral artery. For these patients, bilateral screw insertion through the most posterior and medial part of the isthmus was performed. RESULTS: No massive bleeding or major complications were encountered in any patients with a high-riding vertebral artery. Postoperative computed tomography reconstruction demonstrated that five of the screws cleared the vertebral artery groove successfully, and two slightly breached it. No screws penetrated into the vertebral artery groove. CONCLUSIONS: It is possible to insert a screw safely, even into the narrow isthmus with a high-riding vertebral artery, if the surgeon realizes where the screw should be inserted and has the requisite insertion technique. Bilateral screw fixation should provide a high fusion rate.

Adolescent↗

PubMed2002

Stimulation of bone formation and prevention of bone loss by prostaglandin E EP4 receptor activation.

Bone remodeling, comprising resorption of existing bone and de novo bone formation, is required for the maintenance of a constant bone mass. Prostaglandin (PG)E2 promotes both bone resorption and bone formation. By infusing PGE2 to mice lacking each of four PGE receptor (EP) subtypes, we have identified EP4 as the receptor that mediates bone formation in response to this agent. Consistently, bone formation was induced in wild-type mice by infusion of an EP4-selective agonist and not agonists specific for other EP subtypes. In culture of bone marrow cells from wild-type mice, PGE2 induced expression of core-binding factor alpha1 (Runx2/Cbfa1) and enhanced formation of mineralized nodules, both of which were absent in the culture of cells from EP4-deficient mice. Furthermore, administration of the EP4 agonist restored bone mass and strength normally lost in rats subjected to ovariectomy or immobilization. Histomorphometric analysis revealed that the EP4 agonist induced significant increases in the volume of cancellous bone, osteoid formation, and the number of osteoblasts in the affected bone of immobilized rats, indicating that activation of EP4 induces de novo bone formation. In addition, osteoclasts were found on the increased bone surface at a density comparable to that found in the bone of control animals. These results suggest that activation of EP4 induces bone remodeling in vivo and that EP4-selective drugs may be beneficial in humans with osteoporosis.

Animals↗

PubMed2002

A congenic mouse and candidate gene at the Chromosome 13 locus regulating bone density.

Previously, we identified two significant quantitative trait loci (QTLs) specifying the peak relative bone mass (bone mass corrected for bone size) on chromosomes (Chrs) 11 and 13 by interval mapping in two mouse strains, SAMP2 and SAMP6. The latter strain is an established murine model of senile osteoporosis and exhibits a significantly lower peak relative bone mass than SAMP2 mice. We recently designated the Chr 13 locus as Pbd2 (Peak bone density 2) and constructed a congenic strain, P6.P2-Pbd2(b), which carried a single genomic interval from the Chr 13 of SAMP2 on a SAMP6-derived osteoporotic background. In this study, we have constructed a congenic strain, P2.P6-Pbd2(a), carrying a SAMP6-derived susceptible interval on a SAMP2-derived resistance background. This congenic strain had a lower bone density than the background strain, SAMP2, based on three measurement methods, each utilizing a different principle for evaluating bone density: MD, DXA, and pQCT. Next, a candidate gene approach was used to find polymorphisms of Bmp6 (bone morphogenetic protein 6). The CAG trinucleotide repeat numbers in exon 1 of this gene differ among SAM strains. We found an association of CAG repeat length with relative peak bone mass in mice.

Animals↗

PubMed2002

Cervical disc herniation causing localized ipsilateral popliteal pain.

We report a patient with localized ipsilateral popliteal pain that we believe was caused by cervical disc herniation. A 52-year-old woman complained of an unusual severe aching pain in the right popliteal region. The pain increased gradually for 4 months despite the employment of conservative treatments. Eventually, the woman was unable even to stand immediately after waking because of the pain. However, physical examination of the right knee joint showed little abnormality. Although radiography of the knee joint showed slight osteoarthritic change, the pain remained unexplained. The patient also reported shoulder stiffness and slight numbness in the bilateral toes. Magnetic resonance imaging showed a large disc herniation on the right side of C3/4 and narrowing of the spinal canal at C4/5. Anterior cervical decompression and fusion surgery (C3-5) was performed to prevent impending myelopathy. After surgery the popliteal pain disappeared immediately and completely, suggesting that the cervical disc herniation had caused the pain. The pain has not recurred in almost 3 years after surgery.

Cervical Vertebrae↗

PubMed2002

Use of an aiming device in posterior atlantoaxial transarticular screw fixation. Technical note.

Posterior atlantoaxial transarticular screw fixation is an excellent procedure associated with high fusion rates. There is, however, the potential risk of vertebral artery (VA) injury. The authors designed a special aiming device that allows a cannulated screw to be inserted accurately in the most posterior part of the C1-2 joint via the most posterior and medial part of the isthmus of C-2; this screw pathway most safely avoids VA injury. The instruments include an aiming device and a flexible screw-inserting system. The tip of the aiming device is placed on the ridge of the C-2 isthmus just posterior to the atlantoaxial joint. The guide wire should then pass 1 mm below the device tip. The system consists of flexible guide wires, a drill, a tap, and a screwdriver, and the screw is inserted easily via a posterior approach in which the patient's back is not obstructive. Ten patients with atlantoaxial subluxation or osteoarthritis underwent surgery in which the device was used. In all cases, the screws were inserted safely without causing VA injury, although preoperative computerized tomography (CT) reconstructions revealed a high-risk high-riding unilateral VA in three patients. Postoperative CT reconstructions demonstrated that all screws but one were inserted as planned, and successfully cleared the vertebral groove. In conclusion, this newly designed device is practical and useful for the accurate insertion of screws, thus avoiding VA injury during atlantoaxial transarticular screw fixation.

Aged↗