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Biomedical subjects

N A Atanasov

Publications and source records attributed to N A Atanasov.

11 recordsLinked to original sources
PubMed1987

[Circadian rhythm of uric acid levels of the serum in gout].

Uric acid concentration in the serum was investigated 3 times a day using the uricase method in 15 gout inpatients on a hypopurine diet with age- and sex-adjusted caloric content. The highest uric acid concentration was noted at 7 a. m., the lowest concentration value at 11 p. m. The highest renal clearance values were noted from 7 a. m. till 3 p. m., the lowest ones from 11 p. m. till 7 a. m. Similar regularities were noted in 15 rheumatoid arthritic patients, however uric acid concentration in the serum was lower and its renal excretion was 1.7 times more effective.

Adult↗
PubMed1975

[Polymorphism of water-soluble and triton-X 100-extractable esterases and proteins from Leptospira].

Electrophoresis in gel from polyacrylamide was used to study the water-soluble intracellular esterases, triton-X 100-extracted and proteins of three saprophytic and three pathogenic strains of leptospirae belonging to different serological types. The results of investigation of a relative mobility of 27 fractions with an esterasic activity and the molecular weight of eight of them showed that their polymorphism was mainly consecuent of structural differences. In examination of proteins extracted from the cell residues with triton-X 100 there was also revealed an intertype polymorphism: by acrylamide electrophoresis with sodium dodecylsulphate they were divided into 3--10 fractions with a molecular weight of from 20000 to 200000. The results of these studies are discussed as a manifestation of biochemical individuality of leptospirae of individual serological types.

Bacterial Proteins↗
PubMedDate not supplied

[Determination of the size of protein molecules by means of polyacrylamide gel electrophoresis].

A correlation between the relative electrophoretic mobility of 15 proteins and the concentration of polyacrylamide gel was considered. The extrapolation of straight lines to the zero mobility enabled to determine the effective radius of protein molecules and the extrapolation to the zero concentration of the gel was used to estimate the free electrophoretic mobility. Use of the method for study of protein molecules is discussed.

Electrophoresis, Polyacrylamide Gel↗