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N A Brazhe

Publications and source records attributed to N A Brazhe.

4 recordsLinked to original sources
PubMed2005

Interference microscopy under double-wavelet analysis: a new approach to studying cell dynamics.

This Letter combines a novel experimental approach to the study of intracellular processes with a newly developed technique for multimode time-series analysis. Experiments are performed on isolated pond snail (Lymnaea stagnalis) neurons. Local variations in the cellular refractive index as detected by laser interference microscopy are related to the processes in the cell. A wavelet analysis shows the presence of several identifiable modes in the membrane and intracellular dynamics, and a double-wavelet analysis reveals nonlinear interactions between the regulatory processes in the form of mutual frequency and amplitude modulations.

Animals↗
PubMed2005

[The role of plasmatic erythrocytic membrane in formation of hypoxia in patients with chronic cardiac failure].

AIM: To study oxygen supply to erythrocytes in patients with chronic cardiac failure as a factor provoking hypoxia. MATERIAL AND METHODS: The trial enrolled 12 men with chronic cardiac failure (CCF) of functional NYHA class II aged 37 to 74 years (group A). Group B--healthy donors (n = 10) aged 20-40 years. Conformation changes of hemoporphirine were detected with degradation spectroscopy, viscosity of plasmatic membrane--with resonance spectroscopy. Degradation spectroscopy estimated content of Hb-ligant complexes as 0.34 +/- 0.013; complexes I Hb-NO 0.73 +/- 0.11, complexes II Hb-NO 0.28 +/- 0.08 (group A). RESULTS: Electronic-paramagnetic spectroscopy has estimated: S--0.716 +/- 0.005 (group A), 0.667 +/- 0.011 (group B). In group B- 0.5 +/- 0.08; 0.41 +/- 0.22; 0.55 +/- 0.12, respectively. CONCLUSION: Patients with CHF have high microviscosity of plasmatic erythrocytic membrane near location of polar lipid groups; subnormal content of oxyhemoglobin and elevation of the number of complexes of nitric hemoglobin-oxide; altered binding of nitric oxide with hemoglobin.

Adult↗