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Biomedical subjects

N A Filippova

Publications and source records attributed to N A Filippova.

At least 19 recordsLinked to original sources
PubMed2003

[Effect of medications and acupuncture on indicators of a bioenergetigram of bronchial asthma patients].

AIM: To evaluate the energy informational effects of various treatments in patients with bronchial asthma (BA). MATERIAL AND METHODS: Changes in bioenergograms were analyzed in 139 patients after therapeutical exposures: intravenous prednizolone and dexamethasone, inhaled seretide, fluthicasone propionate, beclomethasone, phenoterol, salmeterol, and salbutamol, the latter drug as tablets, as well as a course treatment with acupuncture. The energy informational effects of pumpan and nitrosorbide were studied in 22 patients with BA concurrent with cor pulmonale and coronary heart disease (CHD). RESULTS: A significant difference was found in the parameters of a bioenergogram depending on the routes of administrations of the drugs and their dosage forms. The greatest and positive changes in the area of the bioenergogram were recorded when using salmeterol and salbutamol, particularly in the nebulization of their solutions, and acupuncture. The intravenous infusion of glucocorticosteroids frequently produced inhibitory effects. In patients with BA concurrent with cor pulmonale and CHD, the positive energy informational effect of pumpan, that differed from that of nitrosorbide, occurred with better ventricular repolarization and alleviated signs of right cardiac overload on ECG. CONCLUSION: The bioenergogram is highly sensitive to changes in the status of patients and it may be used to choose drugs and their combinations, combined drug therapy, and acupuncture on an individual basis. Pumpan is recommended for the treatment of patients with BA concurrent with cor pulmonale.

Acupuncture↗
PubMed1998

Influence of exogenous RAR alpha gene on MDR1 expression and P-glycoprotein function in human and rodent cell lines.

The goal of our study was to obtain direct evidence of co-ordinated regulation of P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) and differentiation in tumour cells and to study some signalling pathways involved in joint regulation of these two cell phenotypes. The sublines of human melanoma (mS) and hepatoma (human HepG2 and rat McA RH 7777) cell lines were obtained by retroviral infection of the wild-type cells with the cDNA of the human retinoic acid receptor alpha (RAR alpha). The resulting sublines stably overexpressed exogenous RAR alpha gene. The infectants became more differentiated than the parental cells as determined by a decrease in the synthesis of the embryo-specific alpha-fetoprotein in HepG2 and McA RH 7777 hepatoma cells and by an increase in melanin synthesis in mS cells. The differentiation of human cells was accompanied by an increase in the amounts of MDR1 mRNA but not by an increase in P-gp activity as a drug transporter, in contrast, in the rat RAR alpha overexpressing cells P-gp functional activity was elevated. Treatment with cytotoxic drug (colchicine) or retinoic acid (RA) resulted in a slight increase in P-gp activity in the parental and RAR alpha-infected melanoma cells, whereas the increase in P-gp function in the infected hepatoma cells (both human and rat) was very prominent. Thus, we provide new evidence that cell differentiation caused by the overexpression of the gene participating in the differentiation programme leads to overexpression of MDR1 gene and drug resistance and that this effect is tissue and species specific. These data imply that the activation of the RA-controlled signalling pathway up-regulates MDR1 gene expression.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1997

Relationships between species of the Rhipicephalus sanguineus group: a molecular approach.

The biosystematic status of species belonging to the Rhipicephalus sanguineus group is difficult to determine by phenotypic methods and has been subject to frequent revisions and ongoing debate. Yet, the differentiation of species within this complex is of clinical importance especially in veterinary medicine due to different host specificities of the transmitted pathogens. In order to generate quantifiable and comparable data independent of environmental influences, a section of the second internal transcribed spacer (ITS2) of the rRNA gene was sequenced for 6 species belonging to the Rhipicephalus sanguineus complex: Rhipicephalus sanguineus sensu stricto, R. turanicus, R. rossicus, R. pumilio, R. pusillus, and R. camicasi. Rhipicephalus sanguineus s. str. and R. turanicus as well as R. pumilio and R. rossicus were found to have a close genetic relationship compatible with possible conspecificities. Conversely, R. pusillus was found to have the most distant relationship to the other species.

Animals↗
PubMed1995

Alterations of melanin synthesis in human melanoma cells selected in vitro for multidrug resistance.

Previous data showing the correlation of multidrug resistance (MDR) and differentiation in tumor cell populations (Melloni et al. 1988; Stavrovskaya et al. 1990) suggest that: 1) isolation of MDR cells by cytostatic drugs leads to the selection of more differentiated cell variants and 2) in more differentiated cell variants the activity of MDR-related P-glycoprotein (Pgp) is more prominent than in less differentiated cells. Here we used human melanoma cell line mS and two variants selected from mS population: a) MDR variant of mS selected by colchicine (mS-0.5) and b) mS-trRAR/2--variant obtained by introduction of expressing retinoic acid receptor RAR-alpha cDNA into mS cell. The differentiation status, expression of MDR1 gene and Pgp functioning were compared in wild-type cells and mS variants. Electron microscopic examination of melanosomes showed that the mS-0.5 subline comprised more differentiating cells in the population than parental mS cultures and that these cells were at later stages of melanogenesis. The increase in the degree of differentiation in mS-0.5 population coincided with MDR1 gene overexpression, occurrence of Pgp molecules on the cell membrane and acceleration of Pgp-mediated Rhodamine 123 (Rh123) efflux. mS-trRAR/2, proved to be more differentiated than mS cells. The MDR1 mRNA level and Rh123 efflux were not elevated in mS-trRAR/2 cells, however, retinoic acid (RA) treatment increased both the degree of differentiation and Rh123 efflux in mS-trRAR/2 to a greater extent than in mS cultures. Thus, the data obtained in this study are in favor of the suppositions mentioned above. The mechanisms of coordinated alterations of differentiation and Pgp activity in MDR cells are discussed.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1995

[Characteristics of the effects of artificial alkalosis on electrical activity of the brain and ultrastructure of blood cells in oncologic patients].

The authors examined 40 patients with malignant tumors of various histogenesis, sites and extent, as well as 5 patients with benign tumors and other non-tumorous diseases. They also studied their electroencephalography and peripheral blood lymphocytic and erythrocytic ultrastructure in metabolic alkalosis temporarily induced by intravenous sodium hydrogen carbonate. In cancer patients without late metastases, alkalosis caused a transient normalization of previously altered electroencephalography, erythrocyte disaggregation and substantially reduced the count of killer cells in small and middle lymphocytes. These findings suggest that patients with malignant neoplasms have a generalized intracellular acidosis which can be temporarily abolished by plasma alkalinization.

Adult↗
PubMed1992

[Dynamics of bioelectric activity of the brain and erythrocyte ultrastructure after intravenous infusion of sodium bicarbonate to oncologic patients].

23 patients with malignant tumors of different location and histogenesis were investigated. There were no metastases in 9 cases. 10 patients had metastases in regional areas and 4--distant. The results were compared with those obtained in 4 patients with nonmalignant diseases. EEG, blood gases, plasma acid--base balance and ultrastructure of erythrocytes were explored before and after intravenous infusion of 4.2% sodium bicarbonate solution. The metabolic alkalosis induced amelioration of EEG, which was changed basically, the condense of pre-membrane layer disappeared or decreased in erythrocytes, and disaggregation of erythrocytes took place in cancer patients vs those with nonmalignant tumors. The results confirm the suggestion of generalized intracellular acidosis in malignant tumor patients. This acidosis can be temporarily avoided or diminished artificially by blood alkalosis.

Acidosis↗
PubMed1992

[Ultrastructural features of synovial sarcomas and their prognosis].

25 synovial sarcomas in patients with various duration of life were studied electron-microscopically. Quantitative correlation between dark and clear tumour cells is the most informative prognostic index in patients operated on because of these sarcomas: the more numerous are dark cells in the tumour, the worse is the prognosis. The appearance of intermediate filaments in the cytoplasm of epithelioid tumour cells and the presence of secretory material in the intercellular spaces are the sign of a less favourable prognosis.

Humans↗
PubMed1992

[Ultrastructural features of benign and malignant lesions of a melanocytic nature].

23 melanomas having the thickness, according to Breslow, up to 1.5 mm and 12 benign melanocytic tumours are studied. Ultrastructural examination gives additional information for differentiating benign and malignant melanocytic tumours. It is necessary to take into consideration such signs as the number and qualitative properties of melanosomes and premelanosomes, structure of nuclear membrane, that of nucleoli, non-specific microfilaments and mast cell infiltration of the tumour stroma. Some ultrastructural difference between lentigo-melanoma and other histological tumour types are noted.

Diagnosis, Differential↗
PubMed1991

[Histiocytic sarcoma: diagnosis and differential diagnosis].

Two cases of clinical diagnosis of tumour of the trachea and tumour of the stomach are described in a 42- and 52-year-old males. Immunohistochemical and ultrastructural methods were used to prove the histiocytic nature of the tumour cells which were morphologically similar to poorly differentiated carcinoma and large cell lymphosarcoma.

Adult↗
PubMed1989

[Morphologic diagnosis of Merkel cell cancer of the skin].

Four cases of this carcinoma have been examined by cytologic and histologic methods and electron microscopy. The tumor may be diagnosed by light optics, and electron microscopy is highly effective here too. Examinations of the tumor and metastases puncture biopsy specimens permit the detection of undifferentiated small-cell carcinoma before the treatment.

Aged↗
PubMed1988

[Malignant myoepithelioma of the breast (histological, immunomorphological and electron microscopic study)].

A rare kind of a breast tumor was identified in a woman of 47. The disease ran a malignant course and presented difficulties for morphological verification. Histological appearance showed areas of spindle-shaped or lamellar cells occasionally encircled with homogeneous hyalin-like substance. Locally, there were structures of alveolar or glandular type with an irregular basal membrane and loose position of cells. The primary histological diagnosis suggested a tumor of mesenchymal origin. Followed by immunomorphologic (monoclonal antibody to KL 1) and electron-microscopic examinations, the diagnosis was verified providing evidence for epithelial nature of the tumor cells, loose, forming glandular-like structures and simulating stromal elements with ultrastructural features typical of myoepithelial differentiation. The evidence obtained justify the tumor definition as a rare neoplasm of the breast known in the literature as myoepithelioma.

Breast Neoplasms↗
PubMed1988

[Merkel cell cancer].

The authors analyze 3 cases of Merkel-cell carcinoma, examined by light and electron microscopy with the use of the immunomorphologic method. The diagnosis of this type of carcinoma is based largely on microscopic examination of the tumor. The principal morphologic sign is the presence of relatively monomorphous cells characterized by a definite growth pattern; trabeculae, strands, separated by connective tissue laminae. Electron microscopy ranks second in informative value; it detects the endocrine granules, provided the number of the examined samples is sufficient. Immunomorphologic detection of cytokeratins confirms only the epithelial nature of the tumor.

Aged↗