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Biomedical subjects

N A Gorbunova

Publications and source records attributed to N A Gorbunova.

At least 19 recordsLinked to original sources
PubMed1992

[Study of humoral hemolytic factors in hemorrhage using chromatographic methods].

The kidney-dependent increase of the haemolytic activity of blood serum after acute decompensated blood loss was demonstrated in the experiments on rats. The preparative ion-exchange chromatography on DEAE-Toyoperal was used to separate the haemolytic active component of the posthaemorrhagic blood serum with the max value of the specific activity of 6.86 A/micrograms protein. The analysis of the separated component by size-exclusion chromatography on TSK-G3000-SW column indicated a molecular mass of 80-100 kDa. In injection in the circulatory system in in vivo experiments the dose-dependent effect of the action of separated component was demonstrated.

Animals↗
PubMed1991

[Study of the effectiveness of artificial oxygen carrier on the hemoglobin base in blood loss replacement in dogs].

The effectiveness of modified polyhemoglobin proposed as an artificial oxygen carrier (AOC) was evaluated in experiments on dogs with acute fatal hemorrhage. It was shown that total oxygen consumption, arterial blood pressure and circulating blood volume were maintained at the normal physiological levels during 4 h after infusion of 10% solution of polyhemoglobin. AOC infusion provided 100% survival of the animals. It has been concluded that hemoglobin polymer modified with pyridoxal-5'-phosphate can be used as the basis for the production of the medicinal form of AOC.

Acute Disease↗
PubMed1991

[Automated system of mass examination of the working population].

The automated system for mass examination and follow-up of therapeutic patients has been developed, tested and introduced with the help of computer family "Electronics" and "IBM". The system is designed for the use at polyclinics, health units and health centres. The following programmes are provided: premedical screening, ECG data interpretation, analysis of physician's data and management of follow-up, primary and secondary prevention of chronic diseases. The application of this system in 2336 persons resulted in substantial saving of physician's time during mass examinations, improved the quality of screening and follow-up.

Diagnosis, Computer-Assisted↗
PubMed1991

[Mechanisms of hemolysis under the effects of extreme factors].

It has been shown that the activation of reno-dependence mechanisms of destruction of the low-stable population of erythrocytes was observed following the acute blood loss, the infusion of the phenylhydrazine and uranyl-glycerine solution. These mechanisms have different nature: humoral (increase of hemolytic activity of the blood serum up to 46.6%), autoimmune-cell or vasorenal (increase of the autoplateformation in 1.8 ones, of the activity of the system mononuclear phagocytes on 37.6%).

Acute Disease↗
PubMed1991

[Various mechanisms of disorders of the regulation of hemostasis in acute hemorrhage].

A review has been presented of the author's own investigations and the literature data on the mechanisms of hemocoagulation disorders in acute hemorrhage. The main attention is paid to the state of the vascular-thrombocytic component of the hemostasis system. In acute hemorrhage, like in the normal state, the blood hemostatic potential is characterized by the combination of parameters of the labile and stable components of the hemostasis system. Under conditions of acute hemorrhage the shifts in the blood coagulation system are directed towards hypercoagulation, and the syndrome of disseminated intravascular blood coagulation may develop. Parameters of the vascular-thrombocytic component of the hemostasis play the main role in the triggering of blood coagulation mechanisms. Accelerated movement of fluid from tissues to the blood flow, disorders in microcirculation and blood rheology, monocytic phagocytes system blocking, toxigenic and hemolytic properties of the plasma play a role in the activation of the vascular-thrombocytic hemostasis.

Acute Disease↗
PubMed1989

[An experimental study of the water balance and acid-base state of the blood during infusions of the plasma substitute oksiamal].

The influence of the plasma substitute oxyamal on shifts in the water and acid-base balance of the blood was studied in experiments on dogs with simulated severe hemorrhagic shock. Oxyamal therapy of the severe hemorrhagic shock led to a stable reduction of the volume of circulating plasma and blood, and to a rapid normalization of blood circulation. No disorders were observed in the fluid distribution in the body, acid-base balance rapidly recovered that has evidenced a full-value transcapillary water-ion exchange.

Acid-Base Equilibrium↗
PubMed1989

[Blood rheologic disorders in patients with polycythemia vera and their correction by therapeutic erythrocytapheresis].

The rheologic blood properties were studied in patients with polycythemia vera (PV) before and after erythrocytapheresis. The patients with PV showed a complex of hemorheologic disorders (high blood viscosity at different rates of deviation, intensified red blood cell aggregation, decreased deformability of these cells) found to be implicated in the disease pathogenesis. Erythrocytapheresis promoted the improvement of the rheologic characteristics such as dynamic blood viscosity and the red blood cell aggregation ratio.

Blood Coagulation Disorders↗
PubMed1978

[Model of disseminated intravascular coagulation].

Vascular-thrombocytic and plasma hemostasis was studied in dogs after blood loss (40--50 ml/kg weight) and subsequent hypervolemic (60--65 ml/kg weight) transfusion of homologous (from 3 donors) platelet and leukocyte rich in plasma. After a short phase of hypercoagulation all the tested dogs displayed hypocoagulation accompanied by a decrease in the count of platelets and a fall of their aggregation function, prolongation of bleeding time, diminution of capillary wall resistance, a decrease of plasma fibrinogen concentration and factor XIII activity, and by a rise of blood fibrinolytic activity. The data obtained show the development of the acute disseminated intravascular coagulation syndrome (DIC). DIC model is porposed on the basis of the results obtained.

Animals↗
PubMed1976

[Dynamics of mental disorders in poisoning with hypnotic and sedative preparations].

In order to study some mental disorders due to poisoning by sleeping and sedative drugs 258 patients with mental and boderline conditions (who had taken these drugs in toxic doses) were studied in the Sklifosovskiy Moscow Research Institute of Emergency Medical Aid. The revealed mental disorders on different stages of poisoning were less differentiated in the acute period (clouded consciousness, coma) and most diverse in pre- and postcomatose stages, where they were characterized by disturbed consciousness, hallucinatory and delusional experiences, epileptiformal syndromes, emotional, motor and other disturbances. The symptomatology depended upon the type of medical drug, intensity of medical procedures, the mental or boderline disease itself which influences the clinical picture of every stage of the intoxication.

Adolescent↗
PubMed1976

[Toxic and hemolytic properties of serum in the massive transfusion syndrome].

Toxic and hemolytic properties of the sera of dogs which received massive homologus blood transfusions were studied in experiments on 20 dogs and 432 mice. The toxicity of the recipient's sera was evaluated in vivo on mice with blocked reticulo-endothelial system and by the method of hemocultures. Red cells labeled with radioactive chromium were used to study the hemolytic activity. Investigations carried out demonstrated the acute blood loss wilth the subsequent massive transfusion of the homologous blood to result in the production in the recipient's serum of toxigenic products. These toxigenic properties were determined by two biotesting methods. The maximal toxigenic action was noted in 2 hours and in the course of the 1st day after the substitution. No toxic effect of the sera was found on the 17th day. A parallel study of hemolytic properties of the sera revealed a marked increase of its activity; it was the greatest during the first 24 hours. In three days the hemolytic properties of the serum were no different from the initial one.

Animals↗