Biomedical subjects
Publications and source records attributed to N A Koreshkova.
Possible use of ethidium bromide immobilized on polyacrylamide gel for DNA extraction from biological media, e.g., cell lysates and blood serum, is discussed. The ability of the affinity gel to cause fractionation of single- and double-stranded DNA is demonstrated.
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The effect of the physiological concentration of cyclic adenosine-3' ,5'-monophosphate (cAMP) analogues on the proliferation of mouse bone marrow stem hemopoietic cells (CFUs) was examined. The stimulating effect was estimated from the decrease in CFUs expressed in the percentage derived from comparing the number of spleen colonies in the control and experimental groups treated with hydroxyurea 10(-3) M (incubation with hydroxyurea resuted in the cell death in S-phase). Cyclic AMP stimulted the proliferation of CFUs by 60%, while its analogues such as 8-(N-chloroacetylaminoethylamino)-cAMP, 1-(N-chloroacetylaminoethyoxy)-cAMP and 1-(N-(p-fluorosulfonyl)-benzoylaminoethoxy)-cAMP stimulated the proliferation by 39.2%, 32.4% and 21.9%, respectively. Therefore, the synthetic analogues of cAMP were not only far from inhibiting the proliferation of CFUs but, on the contrary, exerted a stimualting effect unlike most antineoplastic alkylating drugs that depress hemopoiesis up to its total aplasia.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Fluorometric measurements of the levels of radiation injury to DNA of leucocyte lysates were made on the basis of the estimates of alkaline denaturation rate obtained by the method of Birnboim and Jevcak. Leucocytes of human blood in vitro and rats were exposed to doses of 0.5, 1, 1.5, 3, 6 and 9 Gy. It was shown that the percentage of double-stranded DNA of leucocytes of irradiated human blood decreased with the increase in the radiation dose while that of exposed rats decreased immediately after irradiation and was normalized after 20 h.