Biomedical subjects

N A Robinson

Publications and source records attributed to N A Robinson.

44 records · Page 3Linked to original sources

PubMed1986

Determination of the size of polyphosphates with polyphosphate glucokinase.

A procedure for determining the size of inorganic polyphosphates of chain lengths up to about 750 is described. It involves reducing the size with polyphosphate glucokinase to a chain length that can be accurately determined by polyacrylamide gel electrophoresis. This measurement along with determination of the glucose-6-P formed and the total phosphate of the original polyphosphate permits calculation of the chain length. The accuracy of this method has been demonstrated by comparison with other reliable procedures. Thus far, it is the only method available for sizing long chain polyphosphates with nmol quantities.

Electrophoresis, Polyacrylamide Gel↗

PubMed1984

Polyphosphate kinase from Propionibacterium shermanii: formation of an enzymatically active insoluble complex with basic proteins and characterization of synthesized polyphosphate.

Polyphosphate kinase, which catalyzes the synthesis of polyphosphate from ATP, has been partially purified from Propionibacterium shermanii. The reaction is unusual in that addition of basic protein causes the enzyme to precipitate and the insoluble form has optimal activity. The synthesized [32P]polyphosphate is non-covalently bound to the precipitated material and was isolated from the complex by proteolysis. The gel electrophoresis procedure of Maxam and Gilbert was adapted to sizing polyphosphates. When polyphosphate was treated with alkali, polyphosphates ranging from 1-100 phosphate residues were obtained as individual bands. The untreated enzymatically synthesized polyphosphate migrated as a species in excess of 200 phosphate moieties.

Electrophoresis, Polyacrylamide Gel↗

PubMed1974