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Biomedical subjects

N A Torubarova

Publications and source records attributed to N A Torubarova.

At least 19 recordsLinked to original sources
PubMed2004

[In vitro cell adhesion and integrin expression by calcium ionophore-treated mononuclear cells from patients with acute myeloid leukemia].

As shown elsewhere, cultured acute myeloid leukaemia blasts acquire certain characteristics of dendritic cells upon stimulation with cytokines and calcium ionophore. The ability of leukaemia-derived dendritic-like cells to express immune costimulatory molecules and dendritic cell marker CD83 has been extensively investigated. Although migratory capacity is a major attribute of dendritic cells, the ability of in vitro modified blasts for adhesion, chemotaxis and homing remain elusive. In the present paper, we show that after stimulation with calcium ionophore acute myeloid leukaemia blasts as well as normal dendritic cell precursors demonstrate increased capacity of binding fibronectin and denatured collagen. The expression pattern of integrins on dendritic-like leukaemic cells in general closely resembles that of monocyte-derived dendritic cells, however, variation in cell properties isolated from blood of individual patients are observed.

Acute Disease↗
PubMed1992

[Expression of glucocorticoid receptors and blast cell clearance in children with different variants of acute lymphoblastic leukemia].

Glucocorticoid receptor (GR) levels were estimated in blast cells of 24 children with acute lymphoblastic leukemia, in different immunocytologic subvariants of the disease. No relationship was revealed between immunophenotype and GR number in blasts. It was found that the response to prednisolone therapy did not depend on the number of sites specifically bound to glucocorticoids. The response character is determined not by immunophenotype but by the initial number of blast cells in the peripheral blood (the higher blastosis, the worse response). High blastosis is probably dependent on the tumor growth rate.

Adolescent↗
PubMed1991

[Antiprolifirative effect of chlorbutin and sensitivity of patients with glomerulonephritis to this drug].

Children suffering from different forms of glomerulonephritis were examined for the sensitivity of peripheral blood lymphocytes to the antiproliferative action of chlorambucil in vitro. All the patients were given chlorambucil treatment in accordance with a 4-component scheme. As a result, in 15 patients (group I), a complete clinicolaboratory remission was attained. In 8 patients, (group II), the treatment results were estimated as a partial remission and in 8 patients (group III), the treatment turned out ineffective. In 14 patients of group I, the sensitivity of peripheral blood lymphocytes to the antiproliferative action of chlorambucil was viewed as high whereas in 7 patients of group III as low. Peripheral blood lymphocytes from group II patients had a low sensitivity to chlorambucil. The prognostic value of the laboratory test suggested is under discussion.

Adolescent↗
PubMed1990

[Immunophenotype of peripheral blood lymphocytes in children living in radiation-polluted territories].

The authors provide the data on the assessment of the population composition of peripheral blood lymphocytes. The assessment was performed with the aid of antilymphocytic monoclonal antibodies in children populating radioactive cesium polluted territories and exposed to ionizing radiation in small doses. 56 children were examined. They were distributed into 2 groups depending on radiation exposure: 1 year after the breakdown and 3 years after it. For comparison purposes use was made of the data obtained as a result of examination of 39 children from the unpolluted regions. Measurements were made of the T and B lymphocyte counts as well as of the main subpopulations of T cells. The irradiated children manifested no appreciable alterations in cellular immunity pointing to the status of immune deficiency regardless of the duration of radiation exposure. A moderate reduction of the T-lymphocyte count at the expense of T helpers/inducers may be of nonspecific character.

Accidents↗
PubMed1990

[Membrane markers and interleukin-2 receptor of splenic lymphocytes in children with aplastic anemia: detection of active subpopulations].

A total of 16 children with aplastic anemia were investigated, among them 6 girls and 10 boys, the mean age 10.5 years. Splenectomy was included into the complex of treatment along with glucocorticoid and transfusion-replacement therapy of all the children. The membrane markers of splenic lymphocytes were assayed in the indirect immunofluorescence test with monoclonal antibodies of ICO and OKT series. The mean parameters did not significantly differ from those of subpopulations of splenic lymphocytes in the control group described in the literature. An individual analysis made in 8 patients (50%) has revealed active lymphocyte populations in the spleen: in the group of patients with a favourable prognosis to the disease an increase in the percent of B-cells was recorded in 2, OKT8(+)-lymphocytes in 1 patient; a rise in the percent of cells with receptors to interleukin-2 in 3 patients (from 25 to 32%); in the group of patients with severe aplasia an increase in the percent of activated HLA-DR(+)-lymphocytes was recorded in 2, and OKT8(+)-lymphocytes in 1 patient. All the patients responded well to the treatment. It has been suggested that in all cases the splenectomy effect is associated with the removal of immunoactive lymphocytes and can serve as an equivalent of the immunosuppressive therapy. The effect of splenectomy in the rest cases has been obscure.

Adolescent↗
PubMed1989

[Circulating granulomonocytic precursor cells and phenotype of peripheral blood monocytes in premature infants].

The functional state of granulomonocytic hemopoietic stem cells was investigated in 72 premature infants in the neonatal period. New data were obtained on the concentration of committed granulomonocytic precursors, and on the characteristics of the phenotype of monocytes--the final differentiation component of this series. These data have verified the present notions on the features of nonspecific adaptation reactions in premature newborns.

Cells, Cultured↗
PubMed1989

[Pathogenesis of immunologic deficiency in the initial stage of lymphogranulomatosis in children].

Antilymphocytic monoclonal antibodies of HKO and OKT series were used to study surface antigens of peripheral blood and lymph node lymphocytes in 13 untreated patients with Hodgkin's disease. The purpose of the study was to identify the pathogenetic mechanism underlying the development of immunodeficiency in the early disease stages. The data obtained have demonstrated that the deficiency of T lymphocytes in the peripheral blood was connected with that of mature T cells in the lymph nodes revealed with the use of differential antigens complex. Deficiency of immunologically differentiated T lymphocytes in the lymph nodes combined with high counts of the cells whose membranes carry Ia-like antigen point to the immunologically unfavourable course of the disease.

Adolescent↗
PubMed1989

[Effects of glucocorticoid therapy on membrane markers of lymphocytes and monocytes in children with chronic idiopathic thrombocytopenic purpura].

The treatment with glucocorticoids brings about a decrease in the expression of Ia-like and LFA-J antigens on the membrane surface of peripheral blood lymphocytes and monocytes, diminution of the relative count of monocytes with fixed immunoglobulins (with Fc-receptors). A reduction of the count of B lymphocytes and monocytes with Ia and LFA-J antigens in the spleen was recorded. The hematologic effect attained in the treatment with glucocorticoids was determined by the combined effect of the drugs: by the blockade of monocyte/macrophage receptors and by the redistributiion++ action of prednisolone leading to the release of blood platelets from the bone marrow to the blood bed.

Adolescent↗
PubMed1988

[The expression of lymphocytic function-associated antigen determined by IKO-11 monoclonal antibodies in patients with nephroblastoma and neuroblastoma].

The importance of NK-cells was shown in 12 patients with nephroblastoma and 10 patients with neuroblastoma using ICO-11 monoclonal antibodies. The numbers of circulating LFA-1+ cells were shown to be different in the untreated and treated patients. A certain regular influence of antitumour therapy on the amount of circulating LFA-1+ cells was established.

Adolescent↗
PubMed1988

[Immunocytological characteristics of primary lesions of the lymph nodes in children with lymphogranulomatosis].

Antilymphocytic monoclonal antibodies of UKO and OKT series were used to study surface lymphocytic antigens in 40 primarily affected lymphatic nodes in 19 children with lymphogranulomatosis. In 17 lymphatic nodes immunological reaction was determined as the T-type (OKT3+--42.3 +/- 2.73%); and in 23--the T-cell population depletion was revealed (OKT3+--15.1 +/- 1.37%, P less than 0.001). It was shown that in the course of the disease the depletion of T-cell lymphocytic population comes more rapidly than that of B-cell. It is accompanied by an increase in the number of lymphocytes carring Ia-like antigens on the membrane (UKO-1+).

Adolescent↗
PubMed1986

Clinical effect and some pathogenic mechanisms of high dose monomeric IgG therapy in childhood chronic idiopathic thrombocytopenic purpura.

8 children with chronic idiopathic thrombocytopenic purpura received a high dose therapy with monomeric IgG. Before and after the treatment immunological investigations were carried out. All the children showed a positive clinical response, in 5 children there was an increase of thrombocytes to 111-340 X 10(9)/l. A clinico-haematological effect could be shown in those children with an increased percentage of marrow megakaryocytes with IgG fixed on the membrane before treatment. There was no haematological effect neither in cases with fixed IgG and IgA nor IgM and IgA combined, as well as in the case when there was no fixed IgG on the membrane. A steady clinical effect was provided if the number of bone marrow megakaryocytes with fixed IgG reached the norm. The suppression of the synthesis of antithrombocytic antibodies of the same immunoglobulin class can be regarded as a specific mechanism of high IgG doses.

Child, Preschool↗
PubMed1986

[Surface membrane differentiation antigens of the lymph node lymphocytes in nonspecific lymphoproliferative reactions and lymphogranulomatosis in children].

Monoclonal antibodies ICO and OKT, OKM5, C-ALLA have been used to investigate surface lymphocyte antigens in normal (9), reactive (27) lymph nodes and in lymphogranulomatosis (9) in children. The study has shown that the number of lymphocytes with T and B markers is 45% in normal lymph nodes; OKT4/OKT8 is 0.9. In reactive lymph nodes all lymphocytes have T and B markers; OKT4/OKT8 is, 1.5. T-cell (OKT3+, OKT11+) or B-cell (sIg+, ICO-1+) type differentiation is preferable. B-cell type proliferation with a high number of ICO-1+ lymphocytes appeared in lymph nodes of 4 patients with lymphogranulomatosis. Polyclonal proliferation and differentiation were observed in other 5 children. No correlation between immunology and histologic types of the disease was revealed.

Adolescent↗