The measurement and interpretation of aerobic fitness in children: current issues.
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Adolescent↗
Biomedical subjects
N Armstrong
Publications and source records attributed to N Armstrong.
Potential antimelanomic drugs. I. Synthesis and antimelanomic effect of a spin labelled D,L-amino acid containing a 2-chloroethylnitrosocarbamoyl group.
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Alanine↗
Child-adult differences in whole blood lactate responses to incremental treadmill exercise.
The aim of this study was to evaluate whether fixed blood lactate reference values of 2.5 and 4.0 mmol.1(-1) corresponded to the equivalent intensity of exercise in prepubertal and teenage boys, and men. Twenty six prepubertal boys (mean(sd) age) 11.1(0.4) years), 26 teenage boys (mean(sd) age 14.1(0.3) years), and 23 men (mean(sd) age 22.4(2.7) years) gave informed consent to participate in the study. Oxygen consumption (VO2) and heart rates (HR) corresponding to the 2.5 and 4.0 mmol.1(-1) fixed blood lactate reference values were used as the criterion measures during incremental treadmill exercise. At the 2.5 mmol.1(-1) level there were no significant differences (P > 0.05) in % peak VO2 between groups. For both prepubertal and teenage boys the 4.0 mmol.1(-1) lactate level represented a higher mean % peak. VO2 than for the mean (P < 0.05). The prepubertal and teenage values were again not significantly different (P > 0.05). Factors other than maturation during puberty influence blood lactate responses to exercise.
Adolescent↗
The challenge of promoting physical activity.
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Adolescent↗
Skeletal pattern is specified autonomously by the primary mesenchyme cells in sea urchin embryos.
In the sea urchin embryo the primary mesenchyme cells (PMCs) produce a CaCO3 skeleton in a pattern that is species specific. To establish whether skeletal pattern is specified autonomously by the PMCs or through instructive interactions with the ectoderm, we have taken advantage of improvements in cell transplantation techniques to produce PMC/ectoderm chimeras between two species. It was found that the pattern of the skeletons produced in these chimeric embryos depended upon the source of the PMCs. Thus, skeletal pattern appears to be specified autonomously by the PMCs. In comparing this data with other information known about skeletogenesis, it is proposed that the ectoderm provides spatial and temporal information for the correct placement and timing of skeletogenesis, and that the PMCs then synthesize the skeleton according to an endogenous program. The ability of PMCs from one species to produce an appropriately patterned skeleton in the ectoderm of another indicates that the cues provided by the ectoderm are largely conserved between species.
Animals↗
High and low exercisers among 14- and 15-year-old children.
To investigate ways in which young people's attitudes about, and motivation for, exercise vary with the levels of exercise they take, a self-completed questionnaire was given to 382 children aged 14-15 in two secondary schools in Devon. It was found that the high exercisers (defined by those in the highest quartile) had attitudes to exercise which were more favourable, received more encouragement to exercise and encouraged others to exercise more. The majority of low exercisers had positive beliefs about the value of exercise although approximately half in boys and one-third in girls were satisfied with the amount and kind of exercise they undertook. Low-exercising boys and girls at all exercise levels preferred their exercise to be noncompetitive. The majority of both sexes accepted that their future health depended on their current behaviour and, in girls, strength of this belief was directly related to exercise level. It may be concluded that advice to young people and physical education programmes in schools should take account of the attitudes and beliefs about exercise held by pupils of all physical abilities.
Adolescent↗
Children's task and ego goal profiles in sport.
The purpose of this study was to examine the combined effects of task and ego goal orientations on children's sport motivation. Participants were 115 boys and 116 girls (mean age = 11.12 yrs). Unlike previous research, where the correlates of the two orientations have been examined separately, this study examined the motivational consequences of different task and ego combinations. Four groups were created based on mean splits on the task and ego scales of the Task and Ego Orientation in Sport Questionnaire. These were low task/low ego, low task/high ego, high task/low ego, and high task/high ego. The hi/hi group emerged as the most motivated and the lo/lo group least motivated in measures of sport enjoyment and perceived sport competence. The lo/lo group, made up primarily of girls, appear to be at high risk for non-participation in sport. Children dominated by a task orientation were more motivated than those dominated by an ego orientation. A similar pattern emerged whether children were in the lower or upper third of the perceived sport competence distribution. A high task orientation appears to provide the vital element for sport motivation. The addition of ego involvement to task involvement may enhance sport enjoyment and does not appear to be motivationally detrimental. Task and ego profile analysis seems to offer important insight into the achievement motivation of children of this age.
Achievement↗
Assessment and interpretation of aerobic fitness in children and adolescents.
Our understanding of the development of children and adolescents' aerobic fitness is limited by ethical considerations and methodological constraints. Protocols, apparatus, and criteria of maximal effort used with adults are often unsuitable for use with children. In normal children and adolescents, peak VO2 increases with growth and maturation, although there are indications that girls' peak VO2 may level off around 14 years of age. Males exhibit higher values of peak VO2 than females, and the sex difference increases as they progress through adolescence. The difference between males and females has been attributed to the boys' greater muscle mass and hemoglobin concentration. It appears that boys experience an adolescent growth spurt in peak VO2, which reaches a maximum gain near the time of PHV, but data are insufficient to offer any generalization for girls. Peak VO2 has usually been expressed in relation to body mass, and with this convention it appears that boys' values are consistent throughout the developmental period, whereas girls' values decrease as they get older. This type of analysis may, however, have clouded our understanding of growth and maturational changes in peak VO2, and scaling for differences in body size may provide further clarification. If differences are shown where none were previously thought to exist, then physiological explanations must be sought. Methodological issues have also hindered the understanding of how children's blood lactate responses to exercise develop. The actual lactate level recorded during an exercise test is influenced by the site of sampling and the blood handling and assay techniques. Valid interstudy comparisons can only be made where similar procedures have been employed. In general, children demonstrate lower blood lactate levels at peak VO2 than adults, although individual variation is wide. Therefore the use of blood lactate measures to confirm the attainment of peak VO2 cannot be supported. Exercise at the same relative submaximal intensity elicits a lower blood lactate in children than in adults, but interpretation and identification of developmental and maturational patterns of response are limited by the use of different testing conditions and reference points (e.g., lactate threshold and fixed level reference points). There is growing evidence that the 2.5 mM reference level should be used in preference to the 4.0 mM level, as the adult criterion occurs close to maximal exercise in many children and adolescents. Explanations for child-adult differences in blood lactate responses to exercise are difficult to elucidate.(ABSTRACT TRUNCATED AT 400 WORDS)
Adolescent↗
Cell-cell interactions regulate skeleton formation in the sea urchin embryo.
In the sea urchin embryo, the primary mesenchyme cells (PMCs) make extensive contact with the ectoderm of the blastula wall. This contact is shown to influence production of the larval skeleton by the PMCs. A previous observation showed that treatment of embryos with NiCl2 can alter spicule number and skeletal pattern (Hardin et al. (1992) Development, 116, 671-685). Here, to explore the tissue sensitivity to NiCl2, experiments recombined normal or NiCl2-treated PMCs with either normal or NiCl2-treated PMC-less host embryos. We find that NiCl2 alters skeleton production by influencing the ectoderm of the blastula wall with which the PMCs interact. The ectoderm is responsible for specifying the number of spicules made by the PMCs. In addition, experiments examining skeleton production in vitro and in half- and quarter-sized embryos shows that cell interactions also influence skeleton size. PMCs grown in vitro away from interactions with the rest of the embryo, can produce larger spicules than in vivo. Thus, the epithelium of the blastula wall appears to provide spatial and scalar information that regulates skeleton production by the PMCs.
Animals↗
Promoting physical activity in schools.
Neil Armstrong, director of the Coronary Prevention in Children Project, argues for a comprehensive programme for promoting children's physical activity. The project's survey of adult coronary risk factors in British children revealed a worryingly low level of physical activity among British schoolchildren. Schools are ideally placed to encourage children to take physical exercise, he writes, but parental role models also play an important part.
Adult↗
Abdominal fat deposition in 11-year-old children.
Although the metabolic complications accompanying visceral deposition of fat are well-established, the onset and extent of such fat patterning in children has not been fully documented. This has been due to the problem of computerized tomography exposing children to a prohibitive radiation risk. Nuclear magnetic resonance imaging (MRI) has provided a feasible alternative. Specifically, the purposes of this study were to use MRI (i) to assess the extent of intra-abdominal (IA) and subcutaneous abdominal (SA) fat deposition in 11-year-old boys and girls, and (ii) to identify the most useful anthropometric indicators of IA adiposity in children. Twenty-five boys and 25 girls were selected to represent, by quintiles, the body mass index range for their age. IA fat, SA fat, and total cross-sectional areas were measured from an MRI scan at the umbilicus. Body density was assessed by hydrostatic weighing, and skinfold thicknesses, circumferences and related ratios, and stage of sexual maturity were measured. Results showed that a wide variation in IA fat deposition was present with amounts ranging from 6 to 58 cm2 (mean = 17.8 +/- 10.0) for boys and 15 to 50 cm2 (mean = 24.8 +/- 8.8) for girls. Percentage of cross-sectional area taken up by visceral fat appears to be less than in normal weight adults. Fourteen children had intra-abdominal/subcutaneous abdominal fat ratios that have been associated with higher health risk in obese adults. Waist-hip circumference ratio (WHR), which is widely used as an indicator of IA deposition in adults, was not a useful predictor in these children.(ABSTRACT TRUNCATED AT 250 WORDS)
Abdomen↗
Antilymphoma activity of human gamma delta T-cells in mice with severe combined immune deficiency.
Human Burkitt lymphoma (Daudi) cells grow as disseminated tumors in mice with severe combined immune deficiency (SCID) after either i.v. or i.p. injection. These cells are lysed in vitro by human V gamma 9/V delta 2 T-cells that recognize the groEL homologue on the Daudi cell surface. We report that both Daudi cell-stimulated peripheral blood mononuclear cells (Daudi-PBMC) containing 41-95% of V gamma 9/V delta 2 T-cells and V gamma 9/V delta 2 T-cell clones prolong the survival of SCID mice given inoculations of a lethal dose of Daudi cells. Groups of 6-8-week-old SCID mice were given inoculations i.v. or i.p. of 10(5) Daudi cells followed (through different injection sites) by: (a) 10(7) Daudi-PBMC; or (b) 10(7) unstimulated PBMC; or (c) 0.9% saline solution. All animals in groups (b) and (c) died of disseminated lymphoma, and their survival was significantly shorter than that of mice in group (a) (P < 0.001 for both i.v. and i.p. routes). Significant antitumor effects were also detected when Daudi-PBMC were injected 4 days before or 4 days after Daudi cells (P < 0.05). In vivo depletion of murine natural killer cells by anti-asialo GM-1 rabbit antiserum did not affect survival, suggesting that these cells did not contribute to lymphoma killing. Daudi-PBMC did not exert in vivo antitumor activity against the control Raji lymphoma. Mice receiving i.p. injections of Daudi cells followed by cytotoxic V gamma 9/V delta 2 T-cell clones also survived significantly longer (P < 0.05 for 3 different clones) than animals given Daudi cells alone or Daudi cells followed by noncytotoxic gamma delta T-cell clones. Our results indicate that this model system can be used for studies of human antilymphoma T-cell responses in vivo.
Animals↗
Children's physical activity: an exploratory study of psychological correlates.
Data are now accumulating that show that some children have apparently low levels of habitual physical activity, as measured by heart rate telemetry. However, relatively little is known about the likely correlates or determinants of such activity patterns. The purpose of this study, therefore, was to explore the extent to which activity levels were related to selected psychological factors. The heart rates of 11/12-year old boys and girls (N = 72) were monitored continuously for 12 hr on each of three school days. The same subjects completed psychological inventories assessing physical self-perceptions and motivation. Intrinsic motivation towards physical education and sport was significantly correlated with activity levels for boys. Motivational orientations also predicted activity, but differently for boys and girls. Evidence was found for a discrimination between 'active' and 'less active' girls on the basis of their physical self-perception and motivation scores.
Body Image↗
The influence of the site of sampling and assay medium upon the measurement and interpretation of blood lactate responses to exercise.
UNLABELLED: This paper reports the findings of two investigations into methodological problems associated with the interpretation of blood lactate (BLa) in the sports sciences. In Experiment 1, brachial artery (A), antecubital venous (V) and fingertip capillary (C) blood samples were drawn simultaneously from nine subjects (mean age 21.1 +/- 1.3 years) during an incremental treadmill protocol and immediately assayed for BLa concentration. Experiment 2 investigated the extent of lactate concentration differences in whole blood (WB), lysed blood (LB) and plasma (P) measured using a YSI 23 AM analyser. In Experiment 1, a comparison of the mean BLa concentrations obtained from the three sites revealed no significant differences (P greater than 0.05). Correlations between BLa samples from different sites were very high, with r values ranging from 0.858 to 0.983. In Experiment 2, the mean lactate concentrations were: WB, 4.7 +/- 2.7 mM; LB, 5.0 +/- 3.0 mM; P, 7.0 +/- 3.8 mM. Plasma (P) values were significantly higher than WB and LB. Values from all sites were highly correlated with coefficients ranging from 0.963 to 0.987. IN CONCLUSION: (1) Significant arterial and venous BLa concentration differences do not exist during incremental treadmill exercise. (2) As capillary BLa concentrations reflect arterial values, their use in laboratory and field settings is recommended. (3) Lactate concentration differences in whole blood, lysed blood and plasma will influence the assessment of performance at fixed lactate reference values. (4) If the inter-laboratory test procedures are to be standardized and results compared, precise reporting of lactate sampling and assay techniques is critical.
Adult↗
Are American children and youth fit? Some international perspectives.
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Adolescent↗
In vitro preactivated human T cells engraft in SCID mice and migrate to murine lymphoid tissues.
Mice with severe combined immunodeficiency (SCID) accept grafts of human T and B lymphocytes derived from resting peripheral blood mononuclear cells (PBMC). We wished to determine whether activated human T cells engraft and migrate into lymphoid tissues in SCID mice. PBMC (50 x 10(6)) activated in vitro in a 4-day mixed lymphocyte culture (MLC) were injected into the peritoneum of 12 SCID mice. In 11 of 12 animals killed at 3 or 4 weeks after injection, human cells were detected in cells pooled from lymphoid organs by flow cytometry and by immunohistochemical staining of frozen tissue sections. The percentage of CD45+ cells in the 11 mice ranged from 2% to 45% and the absolute numbers of CD45+ cells recovered from lymphoid organs ranged from 4 x 10(6) to 90 x 10(6). Up to 93% of the human cells expressed the CD3 antigen together with either CD4 or CD8. Human T cells were localized in periarteriolar areas in murine spleens, whereas in the lymph nodes and gut mucosa, the T cells did not show the pattern for T-dependent areas found in human lymphoid tissue. Numerous human plasma cells were detected in the spleen and gut mucosal crypts of engrafted SCID mice. Human IgG was detected in the serum of all 11 engrafted SCID mice. The functional activity of human T cells recovered from murine splenic tissue was very low 3-4 weeks after engraftment.
Animals↗