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N B Azadova

Publications and source records attributed to N B Azadova.

At least 19 recordsLinked to original sources
PubMed1982

RNA synthesis in the L cell-SV5 system.

Comparative analysis of the ribonucleoprotein RNA synthesis was performed in two persistently infected L cell systems. In the first (LSV5-I), cells were infected with the cloned standard SV5 virus, in the second (LSV5-II), infecting virus had been enriched with defective interfering particles (DIP). The LSV5(I) system in its 40th-42nd passages was similar to LSV5(II) at the 2nd-3rd passage levels. There was shown that the ribonucleoprotein 3H-RNA synthesized falls into two classes: the minor corresponding to 50S viral RNA and the major revealing predominantly low molecular RNA. The decrease of the synthesis of the heavy viral RNA fraction and the prevalence of the low molecular RNA promoted the limitation of infection, the survival of cells and prolonged the carrier state. The possible correlation between low molecular RNA synthesis and DIP formation in the L cell-SV5 system is discussed.

Animals↗
PubMed1966

Topography and dynamics of synthesis of structural proteins of Newcastle disease virus.

Zhdanov, Victor M. (The D. I. Ivanovsky Institute of Virology, Moscow, USSR), Nonna B. Azadova, and Leonid V. Uryvayev. Topography and dynamics of synthesis of structural proteins of Newcastle disease virus. J. Bacteriol. 91:1902-1906. 1966.-Newcastle disease virus S and V antigens are synthesized in the cytoplasm, as revealed by the immunofluorescence method. In some experiments, S antigen was found also in the nucleoli. Actinomycin D moderately decreased the titer of infectious virus and V antigen and accelerated the time of appearance of mature virus. Proflavine sharply decreased the synthesis of both antigens and the release of mature virus.

Antigens↗
PubMedDate not supplied

[Integration and transfection of an arbovirus by mammalian cells].

A system: L cells chronically infected with Sindbis virus was studied. Unlike acute infection wherein the mature virions are produced, the chronically infected tissue culture produces subviral structures-infectious ribonucleoproteins. Molecular hybridization experiments revealed the integration of the viral genome (DNA-transcript) into the cellular genome. Transfection experiments showed the possibility to induce the synthesis of the virus in sensitive cells treated with DNA from the chronically infected cells.

Animals↗
PubMedDate not supplied

[Identification of a hemadsorbing agent discovered in uninfected mouse L cell cultures and also the same cultures chronically infected with Sindbis virus].

Electron microscope examinations of continuous lines of mouse L cells, both uninfected (L-init) and chronically infected with Sindbis virus (L-SV) revealed accumulations of ribonucleoprotein strands and virions corresponding by their parameters to paramyxoviruses in the cytoplasms of the cells. Further studies showed L-init and L-SV cell lines to have a manifest hemadsorption effect which could be completely inhibited by antiserum to parainfluenza SV5 virus. Immunofluorescence procedures detected intensive fluorescence in the cytoplasm of these cells which was observed only after treatment of the cells with antiserum to SV5 virus. In response to inoculation of cell homogenates of continuous L-init and L-SV cultures guinea pigs developed antihemagglutinating antibody to simian parainfluenza SV5 virus. On the basis of these results it may be assumed that virus-specific structures and viruses identical by their parameters to paramyxoviruses observed in electron microscope examinations of continuous mouse L-init and L-SV cells are simian parainfluenza SV5 virus.

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