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N Borda

Publications and source records attributed to N Borda.

12 recordsLinked to original sources

[Prevalence of enterohemorrhagic Escherichia coli in a cattle area of Argentina. Genotypic characterization of the strains of animal origin].

BACKGROUND: There is a high prevalence of infection by Enterohemorrhagic Escherichia coli (EHEC) and patients with hemolytic uremic syndrome (HUS) in Argentina. AIM: To study cattle and pigs as a possible reservoir of EHEC in Argentina. MATERIAL AND METHODS: One hundred two healthy animals (68 cattles and 31 pigs) from a livestock in Argentina, were studied. Stool samples were obtained with a rectal swab. The strains were identified by DNA hybridization with specific gene probes detecting Shiga-like toxin 1 and 2 (Stx1, Stx2), and hly gen related to fimbrial adhesin-associated plasmid. EHEC strains were serogrouped using commercial antisera. RESULTS: EHEC was isolated from 30 out of 68 bovines cultures (44.1%) and from 25 out of 31 pigs (58.1%). Isolates carrying genes codifying both Stx1 and Sxt2, were observed in 50% of cattle and 63.9% of pigs. The gene which codifies for hemolysin (associated to fimbrial adhesin) was observed in about 41% of EHEC isolates. Strains belonging to serogroups O26, O111, and O157 were isolated from cattle, and O111, and O157 from pigs. CONCLUSIONS: The high percentage of EHEC in both cattle and pigs and the presence of human infection-associated serogroups, suggests that these animals are a reservoir of EHEC associated with disease in humans.

Animals↗

[Spinal epidural abscess due to Fusobacterium nucleatum].

We report a case of spinal epidural abscess. In our knowledge this is the first ever reported case due to Fusobacterium nucleatum. An 83 year old female patient with a history of lumbar trauma presented severe lumbalgia. Magnetic resonance showed the unusual anterior location, complicated by psoas abscess. Laminectomy and lumbar drainage were performed. She was treated with ceftriaxone plus metronidazole during 10 days. The evolution was favourable without any residual neurological morbidity.

Abscess↗

[Prosthetic valve endocarditis caused by Corynebacterium urealyticum].

Corynebacterium urealyticum has been recognised as causing inflammatory cystitis and other human infections. In our knowledge this is the first case of a prosthetic valve endocarditis due to C. urealyticum. It was diagnosed in a 61 year old male patient with a history of rheumatic fever, hypertension and aortic stenosis. He had undergone surgery to replace the aortic valve and to perform triple aortocoronary bypass. The isolate was not multiresistant. Endocarditis due to C. urealyticum is very rare. Corynebacterium species, usually considered as contaminants, frequently colonize surgical cardiovascular areas and must be taken into account as causative agents of severe endocarditis.

Corynebacterium Infections↗

[Enteropathogenic microorganisms in children with acute diarrhea in 2 hospitals of Rosario, Argentina].

As part of a multicenter collaborative study the relative frequency of enteropathogenic agents in children less than 5 years of age with acute diarrhea was determined. Rates of isolation were similar as regards sex, age, and season. The frequency of polymorphonuclear cells (PMN) in the stools was significantly higher among patients requiring admission in comparison with ambulatory patients. Enteropathogenic E. coli (EPEC) was isolated more frequently in that group in comparison with outpatients (p < 0.001), mainly among children less than 5 months of age. The most prevalent agents were EPEC (26.1%), enterotoxigenic E. coli (ETEC) (9.7%), Shigella (8.5%), Rotavirus (5.1%), Giardia (3.6%), Campylobacter (3.2%), and Salmonella (2.4%). The EPEC predominant serogroups were 0 111, 0 55, 0 26, and 0 119. ETEC serotypes 0 153:H45 and 0 128:H21 were more often isolated. The predominant species in the genus Shigella were S. flexneri (80.5%), and S. sonnei (9.5%); in the genus Campylobacter, the species were C. jejuni (81.3%), and C. coli (18.7%). Shigella was clearly related to the presence of PMN in the faeces, in children less than 5 months old. Campylobacter was more frequent in ambulatory patients more than one year of age. Rotavirus was found predominantly in autumn and winter. Salmonella and ETEC were more frequent in summer. Giardia was associated with weight loss. In about 10% of the cases there were simultaneous mixed isolations of two or more agents. Salmonella isolates were sensitive to the majority of antimicrobial agents probed. Many Shigella and E. coli were resistant to sulfamethoxazole-trimethoprim and ampicillin (40-80%). Nearly all enterobacteria were sensitive to gentamicin and norfloxacin.

Acute Disease↗

Species and serovars of enteropathogenic agents associated with acute diarrheal disease in Rosario, Argentina.

We report the most frequent species and serovars of enteropathogenic organisms in Rosario from 1985 to 1993. Enteropathogenic Escherichia coli was the most prevalent agent affecting 144/570 (25.2%) children; 0111 represented 41.8%, 055: 13.6%, 0119: 12.7%. Among enterotoxigenic E. coli (ETEC) the most frequent were ETEC-ST 0128:H21 and 0153:H45. Shigella spp were isolated in 8.8%; S. flexneri: 7%, principally type 2 (59.5%); S. sonnei: 1.6%, and S. dysenteriae type 2: 0.2%. Campylobacter spp were found in 6.1% of patients; C. jejuni: 4.6%; C. coli: 1.4% and C. lari: 0.2%; except groups 0 13.50 and 0 4 (2 cases each), no predominant serogroups were found. Salmonella was isolated in 2.8% of cases, being the predominant serovar S. typhimurium until 1986, but a dramatically increase of cases due to S. enteritidis was observed since 1987. There was 1.9% of Aeromonas spp and 2 cases due to Vibrio cholerae non 0-1. No Yersinia was found. In patients with gastroenteritis due to Shigella, Campylobacter, Salmonella, or EPEC as the unique pathogen, leukocytes were observed in the faeces in 70%, 50%, 20%, and 10% of cases respectively.

Acute Disease↗

[Hepatic abscess and bacteremia due to Lactobacillus rhamnosus].

The incidence of severe infections caused by Lactobacillus spp. is very low. This paper reports a case of a liver abscess and bacteremia due to Lactobacillus rhamnosus. The patient is a 73 year-old woman with antecedent of diabetes mellitus. She has undergone surgery and received antimicrobial therapy (ampicillin plus gentamicin). The clinical outcome was favorable.

Aged↗

[Bacteremia in 2 hospitals in Rosario, Argentina].

Bacteremia is associated with high morbidity and mortality. Nosocomial bacteremia is associated with an attributable mortality of 35%. In order to characterize the etiological agent of bacteremia cases during two time periods, 6605 patients were studied as follows: 1) from 1980 to 1982 (n = 2401) and 2) from 1988 to 2000 (n = 4204). A total of 596 cases of bacteremia were detected. The most frequent agents were Staphylococcus aureus, negative coagulase (CNS) Staphylococcus, Klebsiella pneumoniae and Escherichia coli. In the second period, an increase in the frequency of bacteremia caused by gram positive bacteria, particularly gram-positive cocci, was noted. Conversely, gram negative bacteria diminished significantly in the second period, specially enterobacterial species. Methicillin-resistant S. aureus and CNS were significantly more frequently isolated in the second period. Infrequent agents of endocarditis, such as Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Corynebacterium urealyticum, were isolated.

Argentina↗

[Distribution of capsular types and antimicrobial susceptibility of Streptococcus agalactiae causing infections in Argentina].

Streptococcus agalactiae is an endogenous bacterium that has emerged in the last 20 years as an etiological agent in both neonatal and perinatal infections, and in immunocompromised patients. The differentiation of the capsular polysaccharide, the presence of surface proteins c, X, R, and molecular methods allow classification in serotypes and genotypes. This identification is a useful tool for epidemiological purposes and virulence studies in this bacterium. The objective of this work was to study the serotypes and the antimicrobial susceptibility of isolates recovered from invasive diseases in different areas of Argentina. In the analyzed sample a fair predominance of Ia and III serotypes was recovered, followed by II and IV serotypes. All the isolates were found to be sensitive to penicillin. A 6% of resistance to erythromycin and a 4.5% to clindamycin were detected. In three of the isolates, constitutive MLS phenotype (resistance to macrolides, lincosamins and streptogramins) was founded, while in the remaining one, inducible MLS phenotype was detected. These results stress the importance of conducting a surveillance of the prevalent serotypes in our country with the goal of future prevention of this disease with an effective vaccine. The knowledge of the antimicrobial susceptibility profile will be also important to obtain therapeutic success in the treatment.

Argentina↗

[Ankle infection].

Explore the source record for details and available documents.

Ankle Joint↗

[Evaluation of a new medium using red-cell lysate for the isolation of Campylobacter jejuni and Campylobacter coli].

A semiprepared medium containing horse globular lysate was evaluated for the isolation of Campylobacter under two incubation atmospheres (5% O2 and candle jar). Studying 19 known strains of C. jejuni, 151 human and 230 animal fecal samples. They were seeded on conventional medium (MC) and on the new medium (MLG) and incubated at 42 degrees C for 24 and 48 h. Campylobacter strains grew better in 5% O2 atmosphere after 48 h of incubation. All the strains that grew or were isolated on MC, could also grow in MLG incubation in candle jar. Campylobacter was isolated from human fecal samples with the same frequency (5.3%) in MC and MLG, independently of the incubation atmosphere employed. These bacteria were isolated in 35.2% and 34.3% of the animal fecal samples seeded on Skirrow modified medium and MLG, respectively. The concordance proportion between both media was 99.13%.

Aerobiosis↗