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Biomedical subjects

N Bowen

Publications and source records attributed to N Bowen.

5 recordsLinked to original sources
PubMed2001

The challenges of innovation in the organization of home enteral tube feeding.

The number of patients discharged from hospital who need home enteral tube feeding has been increasing steadily in the UK. Arrangements for support of these patients is extremely variable. The unsatisfactory arrangements for home enteral tube feeding which existed in Avon in 1996 prompted an innovative reorganization. On the basis of that experience, this review examines the key issues involved and the questions to be considered, which may be of benefit to other trusts faced with similar challenges.

Clinical Competence↗
PubMed2000

Localization, expression and genomic structure of the gene encoding the human serine protease testisin.

Testisin is a recently identified human serine protease expressed by premeiotic testicular germ cells and is a candidate tumor suppressor for testicular cancer. Here, we report the characterization of the gene encoding testisin, designated PRSS21, and its localization on the short arm of human chromosome 16 (16p13.3) between the microsatellite marker D16S246 and the radiation hybrid breakpoint CY23HA. We have further refined the localization to cosmid 406D6 in this interval and have established that the gene is approximately 4. 5 kb in length, and contains six exons and five intervening introns. The structure of PRSS21 is very similar to the human prostasin gene (PRSS8) which maps nearby on 16p11.2, suggesting that these genes may have evolved through gene duplication. Sequence analysis showed that the two known isoforms of testisin are generated by alternative pre-mRNA splicing. A major transcription initiation site was identified 97 nucleotides upstream of the testisin translation start and conforms to a consensus initiator element. The region surrounding the transcription initiation site lacks a TATA consensus sequence, but contains a CCAAT sequence and includes a CpG island. The 5'-flanking region contains several consensus response elements including Sp1, AP1 and several testis-specific elements. Analysis of testisin gene expression in tumor cell lines shows that testisin is not expressed in testicular tumor cells but is aberrantly expressed in some tumor cell lines of non-testis origin. These data provide the basis for identifying potential genetic alterations of PRSS21 that may underlie both testicular abnormalities and tumorigenesis.

Alternative Splicing↗
PubMed1995

Dog parentage testing using canine microsatellites.

Canine microsatellites, comprising one to four base pair repeated sequences, were identified as part of a project to generate a genetic linkage map of the dog. They have been used to assign parentage in a range of doubtful paternity cases, for example, all the pups in a litter from a bitch mated on different days to two different stud dogs were identified by DNA analysis to come from one dog only. DNA typing in another case was able to exclude a male dog as the father of a litter. DNA analysis can be of relevance by allowing pedigree dogs, which would have previously been excluded on the basis of unknown paternity, to be registered with the appropriate kennel club.

Animals↗