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N C Armitage

Publications and source records attributed to N C Armitage.

70 records · Page 4Linked to original sources
PubMed1985

Localization of anti-osteogenic sarcoma monoclonal antibody 791T/36 in a primary human osteogenic sarcoma and its subsequent xenograft in immunodeprived mice.

A primary human osteogenic sarcoma was visualized in situ by external gamma-camera imaging following administration of 131I-labelled anti-osteogenic sarcoma monoclonal antibody 791T/36. A xenograft of the tumour established in immunodeprived mice also showed localization of 131I-791T/36 determined by both gamma-camera imaging and organ distribution studies. Radiolabelled normal immunoglobulin showed no tumour localization. Expression of the 791T/36-defined antigen on xenografted tissue was further confirmed by reaction of its in vitro-cultured cells with fluorescein-labelled 791T/36 antibody.

Animals↗
PubMed1985

Imaging of primary and metastatic colorectal cancer using an 111In-labelled antitumour monoclonal antibody (791T/36).

The monoclonal antitumour antibody 791T/36, previously shown to localize in colorectal cancer when radiolabelled with 131I, has been successfully labelled with 111In. A preliminary evaluation of its imaging potential has been made in fourteen patients with primary and secondary colorectal cancer. Positive localization was achieved in tumour sites of all but one of the patients. The sites of uptake of the 111In-labelled antibody were more clearly defined than when using the same antibody labelled with 131I. The mean tumour to normal tissue uptake ratio per gram of tissue from four resected specimens was 2.8:1. Labelling of the antibody did not affect the tumour uptake of the antibody but facilitated interpretation directly from images without the need for background subtraction.

Aged↗
PubMed1985

Surgical approaches in primary sclerosing cholangitis.

Primary sclerosing cholangitis is an uncommon condition, generally considered to carry a poor prognosis. Based on the distribution of the biliary strictures, treatment groups can be defined. Those patients with localized hilar or predominantly extrahepatic strictures are most likely to be suitable for biliary-enteric bypass and the use of surgical techniques developed for the management of high bile duct strictures are allowing improvement in the results of surgery in this form of the disease. After 3 months to 3 years follow-up (median 16 months) nine of 12 patients treated by surgical biliary decompression are asymptomatic.

Adolescent↗
PubMed1985

The characteristics of blood-borne radiolabels and the effect of anti-mouse IgG antibodies on localization of radiolabeled monoclonal antibody in cancer patients.

Quantitative and qualitative aspects of the blood survival of 131I and 111In-labeled monoclonal antibody 791T/36 have been examined in patients with colo-rectal carcinoma, ovarian carcinoma and osteogenic sarcoma who were receiving labeled antibody in diagnostic immunoscintigraphy trials. The blood clearance of intact antibody radiolabeled with either 131I or 111In was similar. A bi-phasic decline of both radiolabeled preparations was measured with initial half-lives 0.62 and 0.42 days for 131I and 111In labels and then with 1.85 and 1.40 day half-lives, respectively. The Fab fragment of the antibody was lost more rapidly (initial half-life 0.20 days and then 0.78 days). Blood-borne radioactivity was associated predominantly with plasma rather than cellular elements. Radioactivity was still attached to undegraded, uncomplexed, and immunologically active antibody as demonstrated by molecular filtration, immune precipitation, and antigen binding assays. However, anti-mouse-IgG antibody detected within 7 days of administration of radiolabeled antibody was present for at least 10 mo and has implications for the efficiency of repeated image studies.

Animals↗
PubMed1984

The localization of an anti-tumour monoclonal antibody (791T/36) in gastrointestinal tumours.

Fifty-six patients with gastrointestinal cancers and four patients with benign colorectal tumours have been injected with radiolabelled anti-tumour monoclonal antibody ( 791T /36) to assess the degree of localization of the antibody by external scintiscanning and measurements on resected specimens. Twenty-nine patients with primary colorectal cancer showed increased uptake of the radiolabelled antibody in the resected tumours, with a tumour to normal tissue (T:NT) ratio of 2.5:1. All but two of fifteen patients with recurrent or metastatic tumour showed positive images of the deposits on external scintiscanning. Twelve patients with noncolonic gastrointestinal malignancy were studied and in only two patients were tumours demonstrated by external scanning. There were no positive images in four patients with benign colonic disease nor could increased uptake of radiolabelled antibody be demonstrated in the resected specimens. Immunohistology and autoradiography have shown that the antibody can be demonstrated in the pseudoacini and stroma of colon cancer. There are indications that this may represent localization to a cell surface antigen which becomes detached in the processing of the histological sections. It seems that in the gastrointestinal tract the monoclonal antibody 791T /36 is consistently taken up by colorectal cancer. Only a few noncolonic cancers and no benign colonic tumours take up the antibody. This antibody uptake may prove of value in the detection of occult metastases and in the targeting of antitumour agents.

Aged↗
PubMed1984

A study of the reliability of carcinoembryonic antigen blood levels in following the course of colorectal cancer.

Twenty-three patients were studied to assess the reliability of carcinoembryonic antigen (CEA) levels in following the course of colorectal cancer. CEA estimations were made prior to surgery and again postoperatively. The resected specimens were allocated a Dukes' Stage and histological grading (well, moderate or poorly differentiated). In addition, sections were stained for the presence of CEA by an immunoperoxidase method. Of the 23 patients, twelve had either disseminated disease at initial surgery or subsequently developed metastasis/recurrence. Eleven remain disease-free at a minimum follow-up of one year. In all of these the reliability of plasma CEA values in reflecting the disease status has been assessed. No false positive elevations of CEA were found. Three factors emerge as positive predictors of CEA estimation reliability: pre-operative CEA elevation; tumour grading as well differentiated; dark staining for the presence of CEA. These factors identified 15 of the 18 patients (83%) in whom CEA appeared reliable and were not present in any of the five patients where CEA was not reliable. This reliability achieves statistical significance (X2 = 8.5, p less than 0.02). Histological demonstration of CEA may contribute to the reliability placed on plasma CEA estimations and should be considered if serial estimations are to be performed.

Carcinoembryonic Antigen↗
PubMed1984

Immunoscintigraphy of colorectal carcinoma with monoclonal antibody 791T/36.

A monoclonal antibody 791T/36 prepared against a human osteogenic sarcoma has been used to detect primary and metastatic colorectal carcinoma by external imaging of patients infused with 131I-labelled antibody. Positive images were obtained with 8/11 primary colon tumours, 5/12 rectal tumours and 14/15 secondary and recurrent tumours. Positive tumour localization of radiolabelled antibody was confirmed by imaging and radioactivity counts on resected specimens. None of five patients with benign colorectal disease showed positive images and only 2/12 other gastrointestinal cancers were detected.

Animals↗
PubMedDate not supplied

Intervention studies in adenoma patients.

There is considerable evidence that most colorectal carcinomas arise in preexisting adenomas. It also appears that there may be a considerable influence of diet in the genesis and growth of adenomas. This evidence is discussed and the current dietary intervention studies in humans are reviewed.

Adenoma↗