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Biomedical subjects

N C Bhargava

Publications and source records attributed to N C Bhargava.

At least 19 recordsLinked to original sources
PubMed1993

Usefulness of immunoperoxidase test for serodiagnosis of genital chlamydial infections.

An immunoperoxidase test detecting Chlamydia trachomatis specific serum IgG and IgA antibodies, was carried out to determine its efficacy in establishing chlamydial etiology in 104 clinically diagnosed patients attending a major STD Clinic in Delhi. The patients consisted of 58 with nonspecific urethritis/cervicitis (NSU/NSC), 11 with pelvic inflammatory disease (PID), 23 with primary infertility in either male or female and 12 with lymphogranuloma venereum (LGV). IgG antibodies were tested at a dilution of 1:64 and 1:128 and IgA antibodies at 1:16. Although 27.7 per cent (5 of 18) of the controls had IgG antibodies (> or = 1:128), none had IgA, showing the IgA marker as 100 per cent specific. In 80.8 per cent of all the patients, active infection was detected, 81.0 per cent in NSU/NSC, 81.8 per cent in PID, 76.9 per cent in female infertility, 80 per cent in male infertility and 83.3 per cent in LGV patients. The immunoperoxidase test was found to be an extremely simple and rapid test especially suited for laboratories where facilities are limited.

Adolescent↗
PubMed1990

HIV serosurveillance among prostitutes and patients from a sexually transmitted diseases clinic in Delhi, India.

Screening of 701 prostitutes in Delhi for HIV antibodies by a competitive ELISA test and supplementary Western blot test showed that only one of them was infected. Of the 4,572 samples from the patients attending a sexually transmitted disease clinic in Delhi, none showed evidence of HIV infection. In contrast to this low frequency of HIV infection in Delhi, a much higher frequency has been reported to the Indian Council of Medical Research from major metropolitan centers on the coast of India, namely, Bombay and Madras and their surrounding areas. It is possible that the infection is making inroads from these coastal areas to the interior of the country.

Adolescent↗
PubMed1989

Utility of semithin sections in the diagnosis of donovanosis.

The relative merits of tissue smears, paraffin sections and semithin plastic embedded sections in the diagnosis of donovanosis were assessed. In three cases reported negative or doubtful for the organisms by the former two methods, semithin plastic sections provided definite diagnosis. The latter method revealed in addition the organisms in epidermis and perivascular locations. From amongst the three stains: toluidine blue, Leishman's and thionine azure II basic fuchsin, the last mentioned was found to be the method of choice for demonstrating the organisms differently from the host cells.

Calymmatobacterium↗
PubMed1989

An ultrastructural study of donovanosis.

In an ultrastructural study undertaken on donovanosis, the inflammatory response was studied along with the fine structure of causative organisms, Calymmatobacterium granulomatis. Macrophages were seen to be activated and showed presence of numerous filopodia and increase in the number of lysosomes and rough endoplasmic reticulum. Many cells showed vacuoles of varying size in the cytoplasm some of which contained the organisms. Other cells seen included plasma cells, polymorphonuclear cells and sparse lymphocytes. In one case few multinucleated giant cells and dendritic cells with long cytoplasmic processes making cell-cell contact with other inflammatory cells were seen. Such cells have not been described in donovanosis so far. The organisms showed surface structures like pili and vesicles, the role of which is yet to be clearly understood.

Calymmatobacterium↗
PubMed1980

A study of serum protein in leprosy.

Total serum protein albumin, globulin and A/6 ratio were determined in 50 patients of different types of Leprosy and 15 healthy controls. A significant elevation of total serum proteins (P < 0.001) was observed in 25 patients of Lepromatous leprosy and 10 patients of lepra reaction. No statistically significant alteration in total serum protein (P < 0.05) was observed in 15 patients of non-lepromatous leprosy. A significant fall in serum albumin with concomitant rise in serum globulin level (P < 0.001) was observed in non-lepromatous leprosy, lepromatous and patients having lepra-reaction.

Adult↗
PubMed1979

A study of serum fibrinolytic activity in leprosy.

Fibrinolytic activity was studied in 50 patients of leprosy and 30 healthy individuals who served as control. Fibrinolytic activity was determined by measuring euglobulin lysis. No significant alteration in fibrinolytic activity was observed in patients with non-lepromatous leprosy, the levels being approximately similar to control group. However, fibrinolytic activity was found to be significantly decreased in patients of lepromatous leprosy and lepra reaction group. The observed decrease in fibrinolytic activity can be explained on the basis of presence of tissue destruction and vasculitis seen in leprosy more so in patients with lepromatous leprosy and lepra reaction.

Adult↗