Biomedical subjects
Publications and source records attributed to N Clarke.
Glycerol 1:2-cyclic phosphate is released simultaneously with glycerophosphate when kidney glycerophosphinicocholine diesterase (EC 3.1.4.2) acts on glycerylphosphorylcholine and glycerylphosphorylethanolamine. The percentage of cyclic phosphate ester formed is increased at pH values below the optimum and is decreased when Mg2+ or Ca2+ is added to stimulate the reaction.
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1. An enzyme in extracts of mammalian tissues catalyses the hydrolysis of d-myoinositol 1:2-cyclic phosphate (an intermediary in the enzymic degradation of phosphatidylinositol) to produce d-myoinositol 1-phosphate. 2. The enantiomorph of the substrate is not attacked. 3. The pH optimum is about 8.1-8.3 and the reaction is stimulated by Mg(2+) ions. 4. Extracts from rat kidney cortex and medulla are very rich sources of the enzyme; brain, testis and small intestine contain intermediary activities, and other tissues contain very small amounts.
1. On subcellular fractionation of rat kidney homogenates by differential and density-gradient centrifugation, the bulk of the inositol 1:2-cyclic phosphate 2-phosphohydrolase activity remains with the alkaline phosphatase activity, suggesting localization in the brush borders of the proximal tubules. 2. Histochemical studies with a medium containing inositol 1:2-cyclic phosphate and Escherichia coli phosphomonoesterase show Gomori staining around the brush borders of the proximal tubules in the outer cortex only. 3. Serial sections across the kidney from cortex perimeter to papilla suggest that the inositol 1:2-cyclic phosphate 2-phosphohydrolase has a limited distribution along the proximal tubule of the nephron, probably being limited to the pars convoluta, whereas the alkaline phosphatase extends along the pars recta.
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1. A phospholipid that rapidly disappears from pea seeds during the early stages of germination has been isolated and shown to be N-acylphosphatidylethanolamine. 2. Chromatographic evidence for the presence of the same phospholipid in oats, soya beans and spring (tick) beans has been obtained, and its loss during early germination measured. 3. A scheme for the stepwise degradation of the phospholipid with alkali and acid is presented.
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