PubMed Health⌕ Search

Biomedical subjects

N D Heidelbaugh

Publications and source records attributed to N D Heidelbaugh.

At least 19 recordsLinked to original sources
PubMed1988

Hydrated sodium calcium aluminosilicate: a high affinity sorbent for aflatoxin.

Aluminas, silicas and aluminosilicates were evaluated for their ability to sorb radiolabeled aflatoxin B1 (AFB1) from aqueous solution (in vitro). Hydrated sodium calcium aluminosilicate (HSCAS) was selected for testing in vivo due to its high affinity for AFB1, because of its stable association with AFB1, and its GRAS (generally recognized as safe) status as an anticaking agent. The HSCAS, when added to the diet of Leghorn and broiler chicks at a level of .5%, significantly diminished the adverse effects of feeding 7.5 mg AFB1/kg of feed. Thus, this agent (and other aluminosilicate congeners) may prove effective in the preventive management of aflatoxicosis.

Absorption↗
PubMed1986

Determination of deoxynivalenol (vomitoxin) by high-performance liquid chromatography with electrochemical detection.

A rapid method for the analysis of deoxynivalenol (DON) was developed using high-performance liquid chromatography (HPLC) with reductive electrochemical detection (ED). Deoxynivalenol produced by Fusarium roseum growing on solid cornmeal and rice substrates and from naturally contaminated wheat was extracted and quantitated via ED. DON levels in wheat were verified by gas chromatography and structurally confirmed by mass spectrometry. DON was optimally resolved by HPLC employing a radially compressed octadecylsilane column and a mobile phase of deoxygenated methanol-40 mM borate buffer (35:65) at a flow-rate of 1.0 ml/min. Under these conditions DON exhibited an average retention time of 3.6 min. Reductive ED (-1.4 V) allowed a 12-fold increase in sensitivity and greater selectivity than classical UV absorption at 224 nm. A detection limit for DON of 25 pg/microliter was achieved under these conditions. The determination of DON in crude grain extracts was hindered by extractable interfering substances, whereas ED was more functional-group selective (i.e. reduction of the carbonyl moiety). ED permits a direct quantitation of DON from crude grain extracts and may facilitate the determination of this agent and associated metabolites in biological samples.

Chromatography, High Pressure Liquid↗
PubMed1986

Effects of deoxynivalenol in a wheat ration fed to growing lambs.

A wheat diet containing deoxynivalenol (vomitoxin) at 15.6 mg/kg was fed to crossbred lambs for 28 days. Feed consumption, weight gain, and feed efficiency of deoxynivalenol-treated lambs did not differ (P less than 0.05) from those values of controls. Group differences were not noted for hematologic or serum biochemical variables, and gross or microscopic lesions were not observed in treated lambs.

Animal Feed↗
PubMed1985

Toxicity of ochratoxin A and vanadium to growing chicks.

The effects of ochratoxin A (OA) and vanadium (V), singly and in combination, were determined in male Leghorn chicks from 1 to 28 days of age. The chicks were fed a control diet containing the following additives: A) none; B) 2.5 mg OA/kg; C) 50 mg V/kg; D) 2.5 mg OA plus 50 mg V/kg. These data show that body weight gains were significantly reduced by OA and V singly, and a toxicity-enhancing synergism exists between OA and V, which caused a further reduction in performance. The OA-V combination caused a significant increase in the relative weights of the liver, kidney, gizzard, and proventriculus and a significant decrease in the relative weights of the bursa of Fabricius. This decrease in bursal weight was due to atrophy of lymphoid follicles as indicated by the increase in the histologic lesion score. Uric acid in serum was increased, and albumin, calcium, and phosphorus were decreased in the OA-V combination group. There were also small but significant changes in the hematological parameters measured. Distribution of OA to the liver and kidney was not altered, nor was the distribution of V to the liver, kidney, or muscle tissue. Histologic lesions in kidneys were mild but were more prevalent in chicks in the OA-V combination group.

Animal Feed↗
PubMed1973

Microbiological testing of Skylab foods.

The Skylab manned space flight program presented unique food microbiology problems. This challenge was successfully met by careful evaluation of the total Skylab food system by considering the nature of Skylab foods, their processing and handling, and Skylab food safety requirements. Some of the unique problems encountered with the Skylab foods involved: extended storage times, variations in storage temperatures, no opportunity to resupply or charge foods after launch of the Skylab Workshop, first use of frozen foods in space, first use of a food-warming device in weightlessness, relatively small size of production lots requiring statistically valid sampling plans, and use of the food as an accurately controlled segment of sophisticated life science experiments. Consideration of all of these situations generated the need for definitive microbiological tests and test limits. These tests are described in this paper along with the rationale for their selection. Test results are reported which show successful compliance with the test limits.

Aerobiosis↗