ATTACHMENT OF F2 BACTERIOPHAGE TO CELLULOSE NITRATE FILTERS.
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Biomedical subjects
Publications and source records attributed to N D ZINDER.
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A class of host-dependent mutants of RNA-phage f2 are dependent on streptomycin for growth in certain bacterial hosts. Maximum response to streptomycin occurred late in the growth cycle of phage, indicating phenotypic "repair" of a late phage function.
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A bacteriophage is described which grows on recipient (F-) and not on donor ( Hfr, F+, or F') salmonella. The reason for this differential is unknown, since the phage attaches to all mating types, but progeny ensue only from female bacteria. Purification of this phage has been accomplished. A procedure is described for quantitatively determining either the acquisition or loss of the F agent by bacteria.
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Another criterion for the presence of the agent (F) promoting genetic exchange in Escherichia coli was found. It involves a staining reaction of Hfr and F+ coli, but not F- coli, when mixed with strains of Salmonella typhimurium. This reaction was used as a guide in following the transfer of the F agent to Salmonella and back to E. coli. The F agent in Salmonella seems to promote the same kinds of events that it promotes in E. coli.