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Biomedical subjects

N Delasnerie

Publications and source records attributed to N Delasnerie.

13 recordsLinked to original sources
PubMed1978

DNA and RNA antibodies in serum and CSF of multiple sclerosis and subacute sclerosing panencephalitis patients.

DNA and RNA (nucleic) antibodies were found in the CSF of 18 patients with multiple sclerosis (MS) (out of 45), 11 with subacute sclerosing panencephalitis (SSPE) (out of 12) and 9 controls (out of 30). Viral (measles and rubella, by HAI) antibodies were present in all SSPE, 23 MS and 11 control patients. A clear correlation exists between (1) CSF immunological patterns, (2) oligoclonal aspect, (3) simultaneous presence of nucleic and viral antibodies, suggesting the local synthesis of both. This is confirmed by the comparison of the ratios IgG/antibodies in serum and CSF: the CSF ratio may be higher for nucleic and/or viral antibodies in SSPE and MS patients. Thus nucleic antibodies seem to be related to a persistent active infection within the central nervous system.

Antibodies↗
PubMed1978

Serum and cerebrospinal fluid IGM in 203 neurological patients.

Simultaneous serum and CSF IgM determinations were made by electroimmunodiffusion in 5 normals and 203 patients. Absent from normal CSF, IgM was found in 56 patients (27%): (1) A blood-CSF transudation was present in 25 patients (12%). IgM seems of no diagnostic value in these cases. (2) A purely local synthesis was demonstrated in 17 patients (8%), concerning proved (bacterial, viral, parasitic) or supposed (multiple sclerosis) persistent infectious aggressions. (3) In the last 14 patients with IgM in CSF, a transudation was associated with a local synthesis of IgG (meningitis pattern). (4) A ratio IgM .10(3)/albumin above 10 seems an argument for multiple sclerosis.

Encephalitis↗
PubMed1977

Determination of RNA antibodies in serum and unconcentrated CSF by counterimmunoelectrophoresis.

A quantitative counterimmunoelectrophoresis technique has been applied to evaluation of RNA antibodies in serum and unconcentrated CSF. Serum anti-RNA antibodies have been found at high dilutions in some patients with infectious diseases, subacute panencephalitis and multiple sclerosis. RNA antibodies in CSF are significantly linked to oligoclonal aspect, i.e. to a synthesis of IgG inside the CNS.

Antibodies↗
PubMed1977

[Analysis of immune reactions of the CSF. A neuro-immunologic classification (author's transl)].

CSF immunological reactions are classified in 5 patterns, considering the level of CSF albumin and the percentage of CSF-IgG. The 5 patterns are: 1) normal, 2) inflammatory (pure local synthesis of IgG), 3) two types of trasudates (inflammatory or not) when blood-CSF exchanges increase, 4) meningitis if a transudate is associated with local IgG synthesis. The distinction between transudate and meningitis needs the determination of serum IgG. This classification better than the classical ratios, defines the immunological status of a neurological patient in the different phases of his disease. It determines the frequency of the 5 immunological patterns for each nervous disease and the signification of any antibody detected in the CSF. It proposes a common language between the different laboratories and overall, between biologists and clinicians.

Albumins↗
PubMed1977

Oligoclonal aspect in the CSF of multiple sclerosis patients: a statistical and physiopathological study.

Oligoclonal aspect (O.A.) is observed in 51 per cent of 468 MS patients investigated. Elevation of gammaglobulins and lymphocytic pleiocytosis increase significantly its frequency. O.A. is more frequent in presence of an inflammatory transsudate. Clinical aggravation and O.A seem significantly connected. In the first years of the MS, when the course is intermittent, the frequency of O.A. is significantly influenced by the proximity of the last bout. No such differences exist in patients with continuous course. In conclusion O.A. appears as intermittent expression of a local and general immunity reaction, in connection with the progression of the disease and as a very probable manifestation of persistant virus. We proposed 3 years ago in Göteborg a physiopathological hypothesis suggesting multiple sclerosis (MS) is a two-phase disease. In this first paper, we demonstrated the existence of two successive immunological reactions: The first one is an intermittent synthesis of IgG obviously linked to a lymphocytic pleiocytosis which is also intermittent. This firs CSF pattern appears in the same time of a bout and disappears quickly like the clinical symptoms. It may represent the manifestation of a brief and only infectious aggression. After a few years (more than 5 in average) a second mechanism progress slowly: The synthesis of IgG appears continuous, in remission as in aggravation, the lymphocyte count becomes normal and the level of albumin increases progressively. This second pattern suggests, contrary to the first, a continuous auto-immune processus with the intervention of the general (systemic) immunity. The purpose of this second paper is an attempt to elucidate the signification of oligoclonal aspect in relation to this schema.

Female↗
PubMed1975

Simultaneous determination of albumin and IgG in serum and CSF: comparison of electro-immunodiffusion and immunonephelometry.

Simultaneous determination of albumin and IgG has been made on 50 sera and 100 CSF of neurological patients by electroimmunodiffusion (EID) and an automated immunonephelometry (AIN). A new type of gel (mixture of carboxymethyl-cellulose and agarose) was used for the determination of IgG by EID. The statistical comparison between the two methods shows the accuracy and the good reproducibility of automated immunonephelometry.

Albumins↗
PubMedDate not supplied

[Determination of anti-DNA and anti-RNA antibodies in serum and CSF by a new counterimmunoelectrophoresis method].

A new quantitative counterimmunoelectrophoresis technique allows the determination of anti-DNA and anti-RNA antibodies in the serum and the unconcentrated CSF. Normal sera (but not normal CSF) presents anti-DNA and anti-RNA antibodies probably of IgG type. Pathological results (elevated serum values and/or presence of anti-nucleic antibodies in CSF) are observed in some neurological diseases especially SSPE, multiple sclerosis and, generally speaking infectious diseases. A very significant correlation exists between the presence of anti-nucleic antibodies in CSF and a local (intrathecal) IgG synthesis. The same correlation is observed with oligoclonal aspect in CSF. In some cases a percentage of anti-nucleic antibodies higher in CSF than in serum may indicate the persistence of an infectious agent inside the central nervous system.

Antibodies, Anti-Idiotypic↗