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Biomedical subjects

N Dominguez

Publications and source records attributed to N Dominguez.

7 recordsLinked to original sources
PubMed2006

Comparative study between nadolol and 5-isosorbide mononitrate vs. endoscopic band ligation plus sclerotherapy in the prevention of variceal rebleeding in cirrhotic patients: a randomized controlled trial.

BACKGROUND: After variceal bleeding, cirrhotic patients should receive secondary prophylaxis. AIM: To compare nadolol plus 5-isosorbide mononitrate (5-ISMN) with endoscopic band ligation. The end points were rebleeding, treatment failure and death. METHODS: One hundred and nine cirrhotic patients with a recent variceal bleeding were randomized: nadolol plus 5-ISMN in 57 patients and endoscopic band ligation in 52 patients. RESULTS: The mean follow-up was 17 and 19 months in nadolol plus 5-ISMN and endoscopic band ligation groups, respectively. No differences were observed between groups in upper rebleeding (47% vs. 46%), variceal rebleeding (40% vs. 36%), failure (32% vs. 22%), major complications (7% vs. 13.5%) and death (19% vs. 20%), respectively. The actuarial probability of remaining free of rebleeding, failure and deaths were similar in both groups. Time to rebleeding shows that endoscopic band ligation patients had an early rebleed, with a median of 0.5 month (95% CI: 0.0-4.2) compared with patients from nadolol plus 5-ISMN, 7.6 months (95% CI: 2.9-12.3, P < 0.013). Multivariate analysis indicated that outcome-specific predictive factor(s) for rebleeding was Child A vs. B + C (P < 0.01); for failure was Child A vs. B + C (P < 0.02); and for death ascites (P < 0.01) and rebleeding (P < 0.02). CONCLUSION: This trial suggests no superiority of endoscopic band ligation over nadolol plus 5-ISMN mononitrate for the prevention of rebleeding in cirrhotic patients.

Endoscopy, Gastrointestinal↗
PubMed1999

Determination of paroxetine in plasma by high-performance liquid chromatography for bioequivalence studies.

A high-performance liquid chromatographic method is described for the determination of paroxetine in human plasma. Dibucaine was used as the internal standard. Paroxetine was isolated by solid phase extraction using a Bond-Elut C18 extraction column. Separation was obtained using a reversed-phase column under isocratic conditions with fluorescence detection. The sample volume was 500 microliters of plasma. The intra- and inter-assay accuracy and precision, determined as relative error and relative standard deviation, respectively, were less than 10%. The lower limit of quantitation, based on standards with acceptable relative error and relative standard deviation, was 10 ng ml-1. No endogenous compounds were found to interfere. The linearity was assessed in the range 5-100 ng ml-1. Stability of paroxetine during processing (autosampler) and in plasma was checked. This method proved suitable for bioequivalence studies following multiple doses in healthy volunteers.

Antidepressive Agents, Second-Generation↗
PubMed1995

Resistance to transformation by insertionally activated c-erbB is a dominant phenotype in fibroblasts.

Tissue-specific factors influence whether cells are susceptible to transformation by erbB oncogenes. Avian fibroblasts resist transformation by insertionally activated c-erbB (IAc-erbB), whereas erythroblasts are transformed by this mutant of the epidermal growth factor receptor. In studies presented here, NIH/3T3 cells resisted transformation by IAc-erbB. This finding indicates that the nonpermissiveness of fibroblasts is conserved between avian and murine species. Surprisingly, expression of IAc-erbB blocked transformation by a v-erbB allele. The trans-dominant interference by IAc-erbB occurred despite expression at levels lower than for v-erbB. Together with previous reports, the results indicate that IAc-erbB can exert opposite growth effects in different tissues. The switch from positive to negative growth regulation, which depends on the cellular context, provides novel insight into tissue-specific regulation of receptor tyrosine kinases. Evasion of cell-specific inhibition apparently contributes to the distinct tissue tropisms of various erbB mutants.

3T3 Cells↗
PubMed1992

Growing and starving Dictyostelium cells produce distinct density-sensing factors.

Prestarvation factor (PSF) and conditioned medium factor (CMF) are two autocrine factors produced by Dictyostelium cells. Although secreted at different times in the Dictyostelium life cycle (PSF by growing cells and CMF by starving cells), both factors are glycoproteins that are used by cells to measure their own density, and both are important in cell aggregation. To examine the relationship between PSF and CMF, a CMF antisense transformant was tested for the production of PSF during growth. Although this transformant produced extremely low levels of CMF, its production of PSF was essentially normal. We conclude that these two factors are not products of the same gene.

Animals↗
PubMed1987

[Prevalence of lysotype No. 3 of Salmonella paratyphi A in Peru].

Sixty-nine S. paratyphi A strains isolated from Peru were studied with the aid of the phage-typing system of Banker. 55 strains (79.7%) showed specific lytic reactions of the phage-type no 3 and 12 strains (17.3%) those of the phage-type no 1; one strain was atypical and another untypable. All of these strains were susceptible to ampicillin, chloramphenicol, streptomycin, tetracycline and trimethoprim. These results are compared with the data of the scientific literature on the worldwide distribution of the S. paratyphi A phage-types.

Humans↗