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N E Gruntenko

Publications and source records attributed to N E Gruntenko.

At least 37 records · Page 2Linked to original sources
PubMed1998

[Genes, controlling the response of the juvenile hormone degradation system to stress in the Drosophila virilis imago, linked to chromosome 6].

Chromosome localization of the gene controlling the stress response of DFP-sensitive juvenile hormone (JH) esterase in females of Drosophila virilis was conducted. Line 160 of D. virilis carrying recessive markers on all chromosomes was used as a tester. Mature females of line 160 were shown to exhibit a high level of JH hydrolysis; these flies responded to stress by a sharp decrease in JH hydrolysis. Flies from line 147 lacked this response. Thus, mature females of lines 160 and 147 had different levels of JH hydrolysis under heat stress. F2 hybrids that inherited one chromosome pair from flies of line 147 and the remaining chromosomes, from flies of line 147 were analyzed. This analysis showed that the D. virilis gene controlling the stress response of the JH degradation system is linked with chromosome 6.

Animals↗
PubMed1998

[Alkaline phosphatase in Drosophila melanogaster strains differing in male mating activity and their F1 hybrids].

The activity of alkaline phosphatase (AP) was studied in adults of highly active (HA) and low active (LA) Drosophila melanogaster strains and their F1 hybrids, both under normal conditions and after a heat shock (38 degrees C). Under normal conditions, the HA strain expressed a higher AP activity compared to that in the wild-type strain Canton-S and dominated in respect to this character. The AP activity showed a sexual dimorphism, as it was higher in females of both strains. Heat shock (38 degrees C) induced no alterations in the AP activity of D. melanogaster.

Alkaline Phosphatase↗
PubMed1997

Tyrosine decarboxylase and dopa decarboxylase in Drosophila virilis under normal conditions and heat stress: genetic and physiological aspects.

The activity of tyrosine decarboxylase (TDC) and dopa decarboxylase (DDC) was studied in adults of two lines of Drosophila virilis, contrasting in their reaction to stress conditions. Differences were found in the activity of both enzymes between individuals of the examined lines. Genetic analysis of these differences was made. Each of the two enzymes was found to be controlled by a single gene or, possibly, by a block of closely linked genes. The gene responsible for TDC activity is located on one of the autosomes (excluding chromosome II). DDC activity in D. virilis is regulated by a gene located, apparently, on chromosome II. Adults of the line responding to stress by a stress reaction (r-line) were shown to react to a short-term heat stress (38 degrees C, 60 min) by a decrease in TDC activity. TDC activity in flies of the line incapable of the stress reaction (nr-line) did not alter in such conditions. DDC activity of adults of both lines was found to be unchangeable under stress conditions.

Adrenergic alpha-Agonists↗
PubMed1997

[Genetic control of the stress response of the system of juvenile hormone metabolism in Drosophila virilis].

Metabolism of juvenile hormone (JH) in 11-day-old D. virilis flies from resistant and sensitive lines and in hybrids of these lines was analyzed after short-term heat stress (4 h, 38 degrees C). The level of JH hydrolysis after stress remained constant in the sensitive line, but drastically decreased in the resistant one. Between-line differences of the metabolic JH response to stress were shown to be controlled monogenically or by a block of closely linked genes. The allele of the resistant line was dominant.

Alleles↗
PubMed1997

[Genetic analysis of differences in dopa decarboxylase activity between two Drosophila virilis lines differing in dopamine content].

Activity of dopa decarboxylase (DDC) in two Drosophila virilis lines differing in dopamine (DA) content was investigated. The activity was shown to be significantly higher in flies with lower DA content. A possible reason for this phenomenon is discussed. Genetic analysis of the difference showed that DDC activity in Drosophila virilis is controlled by single autosomal gene (or a cluster of tightly linked genes).

Animals↗
PubMed1997

[N-acetylation of biogenic amines in Drosophila virilis].

In adults of two Drosophila virilis lines displaying different genetically determined reactions to stress, N-acetyl-transferase (NAT) activity against substrates dopamine and octapamine was studied. Sexual dimorphism was characteristic of NAT activity when octapamine served as a substrate. However, in the case of dopamine, this phenomenon was not found. This suggests that two different enzymes with N-acetyltransferase activities exist in D. virilis. The flies of the studied lines were not shown to be indistinguishable in both enzyme activities. Short-term stress (38 degrees C for 30 and 60 min) did not affect NAT activity in flies of either studied line.

Acetylation↗
PubMed1997

[Genetic control of Drosophila virilis tyrosine decarboxylase activity].

Tyrosine decarboxylase (TDC) activity was studied in flies of two Drosophila virilis lines having contrasting response to stress. Lines 101 and 147 exhibited different TDC activity. Genetic analysis of these differences was performed. TDC activity was shown to be controlled by one gene or a block of tightly linked genes. The gene controlling TDC activity was mapped to one autosome but not to chromosome 2. In line 101, short-term heat stress (38 degrees C, 60 min) resulted in reduction in TDC activity, whereas, in line 147, this activity remained unchanged.

Animals↗
PubMed1997

[Genetic control of DFP-sensitive juvenile hormone esterase activity in Drosophila virilis].

Genetic analysis of differences in the activity of DFP-sensitive juvenile hormone esterase in 24-hour-old D. virilis flies was performed. Lines of D. virilis resistant and sensitive to long-term heat stress were analyzed under normal conditions. The level of juvenile hormone hydrolysis by this esterase was higher in the resistant than in the sensitive line. Interlinear differences in the enzyme activity were shown to be controlled monogenically or by a block of tightly linked genes. The allele of the resistant line was dominant.

Animals↗
PubMed1996

[Genetic analysis of differences in alkaline phosphatase activity in two Drosophila virilis strains differing in heat stress response].

Genetic control of changes in alkaline phosphatase activity was studied in adult individuals from two strains of Drosophila virilis under normal conditions and at a high temperature (38 degrees C). Flies from strain 101 responded to the heat shock with a reduction in enzyme activity, whereas flies from strain 147 lacked this response. Strains 101 and 147 were shown to differ in alkaline phosphatase activity at a normal temperature. Genetic analysis of these differences was performed. They were shown to be controlled monogenically (or by a battery of tightly linked genes). The gene determining alkaline phosphatase activity was localized on an autosome.

Alkaline Phosphatase↗
PubMed1996

[Genetic control of juvenile hormone metabolism in Drosophila melanogaster].

Juvenile hormone (JH) metabolism in two lines of Drosophila melanogaster adults was studied under normal conditions and after short-time heat stress. Females of the line Cy/L; D/Sb were shown to have high JH hydrolysis levels which to decreased when they experienced heat stress by decreasing its level, Genetic analysis of differences in the level of JH metabolism in lines 921283 and Cy/L; D/Sb of D, melanogaster under normal conditions was performed. It was established that the trait studied was inherited according to the incomplete dominance type. It was shown that these differences were controlled by the gene (genes) localized in chromosome 2.

Animals↗
PubMed1996

[Role of juvenile hormone metabolism in the adaptation of Drosophila populations to stressful environmental conditions].

The effect of heat stress on the system of degradation of juvenile hormone (JH) and on fertility of Drosophila virilis and D. melanogaster was studied. In wild-type strains of both species, stress resulted in delay of oviposition and in a decrease in JH hydrolysis and fertility, which lasted for several days after heat treatment. Experimental repression of juvenile hormone esterase showed that stress response of the Drosophila reproductive system is determined by the decrease in JH hydrolysis. Fertility of Drosophila strains that did not respond to stress by decreasing JH degradation level was not reduced under stress. The role of JH metabolism in Drosophila reproduction under stress and in adaptation of natural Drosophila populations to stressful environments is discussed.

Adaptation, Physiological↗
PubMed1995

[Genetic analysis of differences in metabolism of juvenile hormone in lines of Drosophila virilis resistant and sensitive to heat stress].

The metabolism of juvenile hormone (JH) in imagos of two Drosophila species (D. virilis and D. melanogaster) was studied in the normal state and after short-term heat stress. Females of both species respond to stress by a decrease in the activity of JH esterase. This decrease is realized through a decrease in the amount of protein. JH metabolism in males does not change after heat stress. Genetic analysis of differences in the activity of DPF-resistant JH esterase in stress-sensitive and stress-resistant strains of D. virilis was performed. The differences are controlled by one autosomal gene.

Animals↗
PubMed1995

[Polymorphism of natural Drosophila melanogaster populations by level of juvenile hormone metabolism and reaction to heat stress].

The juvenile hormone metabolism in Drosophila melanogaster females from the laboratory line Canton S and from two natural populations of the Altaiskii krai was studied under normal conditions and under heat shock. The Canton S females were shown to have a high level of juvenile hormone and responded to heat shock by decreasing it. The natural populations were polymorphic for this trait. In both populations examined, flies with a low level of the hormone occurred at high frequencies (56 and 64%). The natural populations were also polymorphic for response of the system of juvenile hormone metabolism to heat shock: flies with a low level of the hormone did not respond to stress by decreasing it.

Animals↗
PubMed1993

[Molecular-genetic analysis of expression features of the evolutionary-conservative neurogene Nc73EF of Drosophila melanogaster].

Molecular-genetic analysis of the Nc (neural conserved) genome sequence of Drosophila melanogaster located at the position 73EF of Drosophila melanogaster was performed. The Nc73EF sequence was shown to be expressed in the nervous system of Drosophila. We constructed the restriction map of this sequence and revealed the main RNA-coding fragment in the 5'-3' orientation. The RNA-dot analysis data demonstrated that expression of the Nc73EF transcripts took place mainly in the nervous system. Hybridization with the human brain poly(A)+RNA confirmed the basic RNA-coding fragment to be evolutionary conservative. Southern blot analysis showed this fragment to be unique in the Drosophila genome. Northern blots detected three transcripts of this DNA fragment.

Animals↗
PubMedDate not supplied

Stress-reactivity and juvenile hormone degradation in Drosophila melanogaster strains having stress-related mutations.

Juvenile hormone (JH) degradation was studied under normal and stress conditions in young and matured females of Drosophila melanogaster strains having mutations in different genes involved in responses to stress It was shown that (1) the impairment in heat shock response elicits an alteration in stress-reactivity of the JH system; (2) the impairment JH reception causes a decrease of JH-hydrolysing activity and of stress-reactivity in young females, while in mature ones stress reactivity is completely absent; (3) the absence of octopamine results in higher JH-hydrolysis level under normal conditions and altered JH stress-reactivity; (4) the higher dopamine content elicits a dramatic decrease of JH degradation under normal conditions and of JH stress-reactivity. Thus, the impairments in any component of the Drosophila stress reaction result in changes in the reponse of JH degradation system to stress. The role of JH in the development of the insect stress reaction is discussed.

Animals↗