Biomedical subjects
Publications and source records attributed to N E Panchenko.
Bovine and human blood plasma contains alpha 2-antiplasmin which possesses affinity to lysin-binding sites in plasmin and inhibits the human plasmin. Its isolation was conducted for two stages: separation of plasminogen on lysin-cellulose, fractionation by ammonium sulphate, desalination on molselector G-25, chromatography on DEAE-Sephadex A-50 and affinity chromatography on plasmin-sepharose with the blocked active site.
Using immobilization of l-lysine on cellulose and CM-Cellulose activated with bromo-cyanogen and dicyclohexyl carboxyimide, respectively, the affined sorbents are obtained and possibility of their application for obtaining the high-purified plasminogen is studied. According to the yield and activity of plasminogen, l-lysine-cellulose and l-lysine-CM-cellulose are not inferior to the known l-lysine-sepharose. With electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulphate the plasminogen preparations obtained by the method of affined chromatography are homogeneous and their molecular mass is 87 000 Daltons.