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N Emi

Publications and source records attributed to N Emi.

98 records · Page 6Linked to original sources
PubMed1983

[Small dose of ARA-C in the treatment of an elderly patient with acute myeloblastic leukemia].

A 79 year old man with a history of myocardial infarction and cerebral infarction was admitted to our hospital in August, 1982. The hematological examination showed anemia and leukopenia (myeloblast 12%), and bone marrow aspiration confirmed the diagnosis of acute myeloblastic leukemia (FAB, M2). Because his general condition was poor, he was treated with small dose of Ara-C (10 mg/m2/12 hr, subcutaneous injections), obtaining complete remission. In cases of acute myeloblastic leukemia in elderly patients where other intensive treatments are contraindicated, it appears to be useful to employ a method of small dose of Ara-C therapy.

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PubMed1983

[Clinical study on the effect of human lymphoblastoid interferon in multiple myeloma].

Ten patients with multiple myeloma were treated with human lymphoblastoid interferon (HLBI). The dosages used were 3 X 10(6) IU to 6 X 10(6) IU of HLBI intramuscularly daily. Out of eight evaluable patients, one partial remission and 3 minor response were observed. More than half patients experienced fever exceeding 38 degrees C and mild myelosuppression. Other toxic effects consisted of anorexia, malaise, liver dysfunction and skin rash. On the basis of our preliminary study, we conclude that HLBI is an effective agent against multiple myeloma.

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PubMedDate not supplied

Inhibitory effect on the establishment of hepatic metastasis by transduction of the tissue plasminogen activator gene to murine colon cancer.

Hepatic metastasis is a major factor in limiting the prognosis of patients with colon carcinoma. Recent investigations indicate a correlation between plasminogen activator profiles and hepatic metastasis. We examined the effectiveness of tissue plasminogen activator (tPA) gene therapy using a hepatic metastasis model of murine colon carcinoma. Murine colon carcinoma Colon 26 cells transduced with an MFGtPA retroviral vector (Colon 26/tPA) or an MFGLacZ retroviral vector (Colon 26/LacZ) were injected into the liver via the superior mesenteric vein of BALB/c mice, whose survival rates were checked daily. The mean survival rate of mice with hepatic metastasis induced by Colon 26/LacZ was 23.1 days, whereas that of mice with Colon 26/tPA was >100 days. The in vitro proliferation of Colon 26/tPA was comparable with that of Colon 26/LacZ, and antitumor immunity to wild-type Colon 26 cells was not induced after an intrahepatic injection of Colon 26/tPA. We suggest that transduction of the tPA gene to murine colon cancer is useful against the establishment of hepatic metastasis.

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PubMedDate not supplied

Reduction of interleukin-2-receptor expression on retroplacental blood lymphocytes in human pregnancy.

Retroplacental blood lymphocytes (RPL) in human pregnancy were studied for proliferative response induced by recombinant interleukin-2 (rIL-2) and anti-Tac positive cells before and after PHA stimulation for 24 hr and compared with peripheral blood lymphocytes (PBL) of the same donor. Proliferation of RPL induced by IL-2 was significantly lower than that of PBL in all of six cases. In addition, flow cytometry analysis of IL-2 receptors (IL-2R) revealed that IL-2R expression of RPL after stimulation with PHA for 24 hr was significantly less than that of PBL in three of four cases and that RPL showed significantly fewer IL-2R than PBL even at resting state in one case. These results suggest that RPL seem likely to be composed of reduced numbers of lymphocytes bearing IL-2R of non-Tac protein and to have impaired capacity for the acquisition of high-affinity receptors for IL-2.

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PubMedDate not supplied

Gene transfer with cationic lipid into human hepatocellular carcinoma in nude mice.

BACKGROUND/AIMS: Using a cationic lipid, gene transfection into the tumor of a human hepatocellular carcinoma model in nude mice was attempted in order to explore the possibility of its use in gene therapy. METHODOLOGY: A DNA-lipid complex was made by combining the cationic lipid distearyldimethyl ammonium bromide (DDAB) with pCMV sPORT expressing the reporter gene LacZ. The expression of this complex was first investigated in vitro against the human hepatocellular carcinoma cell line Li7HM. It was then injected directly into a hepatocellular carcinoma model tumor created by implanting Li7HM into the liver of BALB/c nu/nu mice, and the expression of LacZ was histologically evaluated. RESULTS: LacZ gene was expressed in Li7HM in vitro with the optimized DNA-lipid complex. Cell toxicity was not a problem. Expression of LacZ was also seen in the mouse hepatocellular model tumor into which the complex had been injected, indicating successful gene transfection with this method. CONCLUSIONS: Direct injection of a DNA-lipid complex into a mouse hepatocellular carcinoma model tumor is a safe and simple method of gene transfection, proving this to be a viable method of transfer for use in gene therapy.

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PubMedDate not supplied

Inhibition of establishment of hepatic metastasis in mice by combination gene therapy using both herpes simplex virus-thymidine kinase and granulocyte macrophage-colony stimulating factor genes in murine colon cancer.

Herpes simplex virus-thymidine kinase (HS-tk) gene therapy with ganciclovir (GCV) treatment has been reported to inhibit the tumor growth, which is applied to the gene therapy targeted to the malignant brain tumor. To suppress the tumor growth completely, the authors designed the HS-tk gene therapy in combination with granulocyte macrophage-colony stimulating factor (GMCSF) gene using the hepatic metastatic model of murine colon cancer. The transduction of the HS-tk gene in combination with the GMCSF gene, followed by GCV, showed a complete inhibition of hepatic metastases of murine colon cancer, which was significantly superior to that of HS-tk gene alone. The growth of cancer cells transduced with both HS-tk and GMCSF genes was inhibited in vitro, and long-lasting antitumor immunity after hepatic metastasis of cancer cells transduced with both HS-tk and GMCSF genes was acquired. It is suggested that HS-tk gene therapy in combination with the GMCSF gene is effective for the complete inhibition of hepatic metastasis of murine colon cancer.

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