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Biomedical subjects

N F Besch

Publications and source records attributed to N F Besch.

17 recordsLinked to original sources
PubMed1983

Evidence of a possible direct action of Danazol on the human ovary.

Strong evidence is presented that Danazol might exert a direct action on the human ovary by binding to receptor-like receptors for progesterone and testosterone, in concentrations well within the pharmacological range of the treated patient. The possible significance of this finding in understanding Danazol's mode of action is discussed.

Adult↗
PubMed1982

Unconjugated steroids in leiomyomas and tumor-bearing myometrium.

The concentrations of 17 beta-estradiol, progesterone, and estrone in leiomyomas and tumor-bearing myometrium were determined by specific radioimmunoassays in hysterectomy specimens from 12 women aged between 30 and 47 years. The concentration of 17 beta-estradiol was significantly higher in leiomyomas than in myometrium. There was no difference in the concentration of progesterone in leiomyomas and myometrium. The concentration of estrone was higher in the myometrium than in the leiomyomas, although the difference was not statistically significant. The concentrations of progesterone, 17 beta-estradiol, and to some extent estrone show variation with the phase of the menstrual cycle. The significance of the difference in concentration of 16 beta-estradiol in leiomyomas and myometrium, the relationship of this difference to the concentrations of estrogen and progesterone receptors, and factors possibly related to the growth of leiomyomas are discussed.

Adult↗
PubMed1982

Evidence for an androgen binding component in human placental cytosol.

A high-affinity, low-capacity androgen binding component is present in human term placental cytosol. A synthetic androgen tracer, [3H]-R1881, was bound by a single class of binding sites with a K4 of 2.73 +/- 1.25 X 10(-9) M and a concentration of binding sites of 157 +/- 73 fmol/mg protein (N = 20). In competitive binding studies, R1881, T, 5 alpha T, androstenedione and DHEA, were active competitors, but not estradiol, progesterone or cortisol. The [3H]-R1881 macromolecule complex exhibited a sedimentation coefficient of 4-5 S under low and high salt conditions, and the radioactive tracer was displaced by a 100-fold excess of unlabelled R1881. This binding component has characteristics of high-affinity, low-capacity, sedimentation behaviour and specificity, commonly attributed to androgen "receptors".

Androgens↗
PubMed1981

Estradiol and progesterone binding in human term placental cytosol.

Human term placentas were studied with regard to their ability to bind estrogens and progestins. A proteinaceous molecule was demonstrated in the high-speed supernatant fraction of disrupted cells that bound estradiol and sedimented in a sucrose density gradient with a coefficient of variation of 4 to 4.5 S with respect to bovine serum albumin. Estradiol was also bound by a single class of binding sites with a dissociation constant (Kd) of 7.35 +/- 1.10 X 10(-9) M and a concentration of binding sites of 54.67 +/- 17.62 fmol/mg protein (N = 7). A synthetic progestin tracer, R5020, was used in an exchange assay to detect any progestin binding and a Kd of 8.13 +/- 1.07 X 10(-9) M, and number of binding sites of 35 +/- 10 fmol/mg protein (N = 6) were obtained at 15 degrees C for 2 hours. No binding was observed in sucrose density gradients. The apparent specificity of the receptor protein for estrogens and progestins was demonstrated by means of competition studies. The possible role of these macromolecules in the human placenta is suggested.

Centrifugation, Density Gradient↗
PubMed1980

Receptor-like binding proteins for testosterone and progesterone in the human ovary.

The presence of two receptor-like proteins in human ovarian cytosol is described, one for testosterone and the other for progesterone. They have high affinity and are saturable, thermolabile, and highly specific. The properties of these proteins are discussed, and their possible role in the regulation of ovarian function is discussed.

Adult↗
PubMed1979

Inhibition of gonadotropin by delta9-tetrahydrocannabinol:mediation by steroid receptors?

Competition assays for estradiol receptors in cytosol preparations of uteri from rhesus monkeys and humans showed that delta9-tetrahydrocannabinol (THC) does not compete with estradiol for intracellular estrogen recptors. Although isotopically labeled THC bound to macromolecules in uterine cytosol from the rhesus monkey, the binding was not displaced by unlabeled THC, diethylstilbestrol, estradiol, progesterone, cortisol, or 5 alpha-dihydrostestosterone. Scatchard analyses indicated that high-affinity saturable binding of THC to cytosol did not occur. Thus the inhibitory effect of THC on gonadotropin and steroid secretion in primates is not mediated by the interaction of THC with intracellular steroid hormone receptors.

Animals↗
PubMed1979

Effect of tetrahydrocannabinol on the hypothalamic-pituitary axis in the ovariectomized rhesus monkey.

Single doses of delta9-tetrahydrocannabinol (THC) (5.0, 2.5, 1.25, or 0.625 mg/kg) can decrease the levels of both luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the ovariectomized rhesus monkey. The inhibition of gonadotropins (50% to 88%) lasts for 6 to 24 hours depending upon the dose of THC. There are no great differences in the responses of the two gonadotropins to THC. The inhibition of gonadotropin levels by THC appears to be at the level of the hypothalamus, since both LH and FSH are released from the pituitary gland in response to LH- releasing factor in the presence of THC.

Animals↗
PubMed1977

The effect of marihuana (delta-9-tetrahydrocannabinol) on the secretion of luteinizing hormone in the ovariectomized rhesus monkey.

Despite the widespread use of marihuana by large numbers of young adults in the reproductive years, little is known about the effects of this drug on reproductive function. information on the effect of the drug on the woman is particularly meager. The rhesus monkey provides an excellent animal model from which to generalize to man. While the primary purpose of the project is to determine the effect of long-term administration on the cycling female monkey, the initial studies were carried out in the ovariectomized monkey with resulting elevated gonadotropin levels under conditions of short-term administration of the drug. Marihuana causes a reversible depression in luteinizing hormone levels in the ovariectomized female rhesus monkey.

Animals↗
PubMed1975

Total urinary estrogens in complicated pregnancies.

One hundred fifty-seven pregnancies complicated by different degrees of diabetes, toxemia, and hypertension were studied with serial urinary placental estrogen determinations. A simple and fast method for total placental estrogen determination was used. The level of total estrogen excretion was related to Apgar score in cases of class B diabetes, severe toxemia, and also in moderate toxemia when estrogen excretion was falling. Mean estrogen levels did not differ as a function of severity of diabetes. Levels did differ with severity of toxemia; however, only the difference in mean estrogen excretion between mild and severe toxemia was significant. Estrogen excretion was very low in hypertension but was not related to Apgar score. This study concludes that total urinary estrogens constitute only a single parameter necessary in the management of high-risk pregnancies.

Apgar Score↗
PubMedDate not supplied

Saliva testosterone and criminal violence in young adult prison inmates.

Free testosterone was measured in the saliva of 89 male prison inmates. Inmates with higher testosterone concentrations had more often been convicted of violent crimes. The relationship was most striking at the extremes of the testosterone distribution, where 9 out of 11 inmates with the lowest testosterone concentrations had committed nonviolent crimes, and 10 out of 11 inmates with the highest testosterone concentrations had committed violent crimes. Among the inmates convicted of nonviolent crimes, those higher in testosterone received longer times to serve before parole and longer punishments for disciplinary infractions in prison. In the housing unit where peer ratings were most reliable, inmates rated as tougher by their peers were higher in testosterone.

Adolescent↗