PubMed Health⌕ Search

Biomedical subjects

N F Gamaleia

Publications and source records attributed to N F Gamaleia.

At least 19 recordsLinked to original sources
PubMed2006

Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma.

AIM: To study circadian rhythms (CR) of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma were compared with those in healthy men. METHODS: The NK-cell and phagocyte cytotoxic activity in five patients with malignant melanoma stage I or II and 12 healthy donors has been assessed by radioimmune assay and NBT-test. RESULTS: The circadian rhythmicity in NK-cells and phagocyte activity in all cancer patients under study has been disrupted. The extent of such disruption tended to increase in patients with more advanced cancer. The most typical alterations were discoordination between the cytotoxicity rhythms of NK-cells and phagocytes (synchronized in healthy persons) and alterations in basic rhythm parameters: phase shifts and amplitude damping. CONCLUSION: In melanoma patients the significant alteration of CR in NK-cells and phagocytes cytotoxic activity was revealed. In spite of individual variations, the degree of the rhythm disruption basically depended on a disease stage. The alteration of CR phase and amplitude and discoordination between the rhythms of NK-cells and phagocyte were registered in all cases studied.

Circadian Rhythm↗
PubMed2005

Effect of photodynamic therapy on tumor angiogenesis and metastasis in mice bearing Lewis lung carcinoma.

AIM: To investigate the influence of photodynamic therapy (PDT) on the tumor angiogenesis and metastasis. METHODS: The effect of PDT with a photosensitizer 5-aminolaevulinic acid (ALA) on tumor metastasis and angiogenesis was studied on the model of subcutaneously implanted Lewis lung carcinoma (3LL) in C57Bl/6 mice. VEGF level in blood serum of animals was evaluated by ELISA. RESULTS: It has been shown that application of ALA-PDT resulted in the lowered rate of metastatic spreading and decreased VEGF level in blood serum of 3LL-bearing mice, and morphologic alterations of vascular system in tumor tissue. CONCLUSION: The antitumor action of PDT using ALA as a photosensitizer is caused partly by antimetastatic and photodamaging effect mediated by vascularization disturbances in tumor tissue.

Aminolevulinic Acid↗
PubMed1989

[Use of low-energy laser radiation in experimental and clinical oncology].

Experimental data concerning the action of low-energy (nondamaging) laser radiation on the malignant cells in vitro and on animal tumours are reviewed. It is shown that under certain conditions the helium-neon and helium-cadmium laser radiation inhibits the tumour cell growth. This inhibition may be mediated by the activation of the organism protection mechanisms. The encouraging results of the application of the low-energy laser radiation in the complex treatment of oncological patients are presented.

Animals↗
PubMed1988

[Preservation of the circadian rhythm of human lymphocytes under in vitro conditions].

The proportions of E-rosette-forming cells on the 9th, 13th, 17th, 21st, 1st and 5th h were determined in the blood of healthy donors divided into 3 groups (4 men in each). I--the blood was taken at every specified time point and the rosette formation was tested immediately; II--the blood was taken at hour 9, stored at +20 degrees C throughout the experiment and used for the tests at the same time points; III--the blood taken at hour 9, the mononuclear cells were obtained and kept throughout the experiment for the determination of rosette formation at the same time points. In all the three groups similar circadian curves were obtained with a peak at 13th and a minimum at the 1st hour. It is believed that lymphocytes possess oscillator(s) of the activity of receptors that are used for cell subset identification.

Adult↗
PubMed1988

[Experimental validation and the initial experience of the use of intravenous laser irradiation of the blood in oncology].

The treatment of some non-oncological and then oncological patients was undertaken on the basis of the results obtained by laser blood irradiation (a helium-neon laser, the wavelength of 0.633 micron) in the tumour-bearing mice. The method was applied to 25 patients with the cancer of cervix uteri after their radiotherapy and resulted in the stimulation of their hemopoietic and immunologic systems.

Animals↗
PubMed1985

[Selective action of argon laser radiation in breast cancer].

The attempts were made to apply the argon laser radiation (the green region of light spectrum) for the treatment of breast carcinoma. The 46 post-operative skin metastases of breast carcinoma in 15 women were irradiated with no photosensitizers used. As a result of the treatment (4 to 50 sessions) the complete resolution of metastatic tumours was achieved in 13 patients. In contrast to the methods of laser coagulation treatment of tumours, used till now, the described approach provides for selective elimination of tumour cells without normal tissue alteration. The involvement of photothermic or photochemical mechanisms in this selective effect is discussed.

Breast Neoplasms↗
PubMed1985

[Laser therapy of cervix dysplasia].

Irradiation from a continuous wave CO2-laser was used in the treatment of 1,234 cases of cervical epithelial dysplasia. Diagnosis was established on the findings of extended colposcopy, aiming biopsy and cytological examination of smears. Such advantages over diathermocoagulation as complication-free recovery and a low incidence of recurrences (2.6%) were shown. The end results were studied.

Adult↗
PubMed1978

[Treatment of skin tumors with a pulse laser].

The clinical laser complex including neodymium and CO2--lasers is described, and the methods of irradiation depending on the type of the laser and tumor are given. The results of the treatment in 559 patients with different malignant and benign skin tumors are analysed. The data of the follow-up (up to 7 years) indicated that 15 of 48 patients with primary melanomas developed metastases, and 12 of them died. Among 105 patients with skin cancers 4 persons had recurrences and were irradiated repeatedly. The main merits of the method compared with surgery and X-ray therapy (the absence of side effects, promptness of the treatment, sterility and precise localization of the effect, vascular thrombosis contributing to an ablastic operation) are discussed. The results obtained with neodymium and CO2--lasers are compared.

Adolescent↗