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Biomedical subjects

N F Moore

Publications and source records attributed to N F Moore.

At least 19 recordsLinked to original sources
PubMed1989

Bunyaviridae. Serological relationships.

The family Bunyaviridae comprises 5 genera of lipid-enveloped viruses with trisegmented RNA genomes. One of the genera, Bunyavirus, comprises over a quarter of the known arboviruses. The members of the Hantavirus genus are apparently the exception in that they are not arthropod-borne. The genera are sorted largely on serological grounds. The Nairoviruses, in addition to the presence of the physical marker of a higher molecular weight nucleoprotein, include Congo-Crimean haemorrhagic fever, an apparently isotypic virus of wide distribution.

Animals↗
PubMed1987

A reovirus from the bedbug, Cimex lectularius.

Large numbers of virus particles were identified by electron microscopy in the epithelial cells of the ventriculus of the bedbug, Cimex lectularius. The morphology of the virus particles and the presence of a segmented double-stranded RNA genome imply that this isolate should be included in the Reoviridae.

Animals↗
PubMed1987

Cricket Paralysis Virus, a Potential Control Agent for the Olive Fruit Fly, Dacus oleae Gmel.

Representatives of several families of insect viruses were tested for growth and pathogenicity in the olive fruit fly, Dacus oleae Gmel. The viruses included nuclear polyhedrosis viruses, an iridovirus, two picornaviruses, and Trichoplusia ni small RNA virus (a member of the Nudaurelia beta family), in addition to two naturally occurring viruses of the olive fruit fly. Two viruses, one of the two picornaviruses (cricket paralysis virus [CrPV] and the iridovirus (type 21 from Heliothis armigera), were found to replicate in adult flies. Flies which were fed on a solution containing CrPV for 1 day demonstrated a high mortality with 50% dying within 5 days and nearly 80% dying within 12 days of being fed. The virus was transmissible from infected to noninfected flies by fecal contamination. The CrPV which replicated in the infected flies was demonstrated to be the same as input virus by infection of Drosophila melanogaster cells and examination of the expressed viral proteins, immunoprecipitation of the virus purified from flies, and electrophoretic analysis of the structural proteins.

Journal Article↗
PubMed1987

Measurement of surface charge of baculovirus polyhedra.

The isoelectric points of three baculoviruses, Trichoplusia ni nuclear polyhedrosis virus (NPV), T. ni granulosis virus, and Spodoptera littoralis NPV were identified by cell electrophoresis. At neutral pH polyhedra were negatively charged. T. ni NPV polyhedra were reacted with a number of reagents which could potentially attach to or degrade their surface structure. This gave information on the components that contribute to the charge profile of T. ni NPV. This is discussed in relation to the use of polyhedra as biological control agents against insect pests.

Journal Article↗
PubMed1987

An inapparent infection with a probable picornavirus in several stocks of laboratory reared and naturally occurring populations of Dacus oleae Gmel. pupae in Greece.

Pupae from several stocks of wild and laboratory reared olive fruit fly, Dacus oleae, were fractionated by a series of steps designed to identify occluded and nonoccluded viruses. Two different size of particles were isolated, the smaller of which contained a single-stranded RNA molecule of about 2.8 x 10(6). This small RNA virus was found to inapparently infect a Ceratitis capitata continuous cell line.

Animals↗
PubMed1987

Investigation of virus-like particles in Leishmania hertigi.

The promastigote forms of an isolate of Leishmania hertigi contain large numbers of virus-like particles (VLPs) in paracrystalline arrays. The VLPs are not present in the amastigote form and it was not possible to purify them from large amounts of the promastigotes.

Animals↗
PubMed1986

Proteins expressed by Mill Door/79 virus, a Kemerovo serogroup orbivirus transmitted by the ticks Ixodes uriae.

Mill Door/79 virus, an orbivirus of the Kemerovo serogroup, Great Island Complex, was shown to induce in infected cells 10 segments of double-stranded RNA with a total molecular weight of 11.46 X 10(6) daltons. Using methyl mercuric hydroxide as a denaturing agent the double-stranded RNA was translated in a cell-free system producing 11 polypeptides, 10 of which co-migrated with those produced in Mill Door/79 virus infected Vero cells. The segments were separated and individually translated in a cell-free system allowing their coding assignments to be made. These assignments were confirmed by partial proteolysis.

Animals↗
PubMed1986

Hydrophobic interactions involved in attachment of a baculovirus to hydrophobic surfaces.

The hydrophobic interactions of Trichoplusia ni nuclear polyhedrosis virus were characterized by hydrophobic interaction chromatography. The determination of the hydrophobic force and some of the factors that influence its size is discussed in relation to the attachment to leaf surfaces of polyhedra during their use as biological control agents against insect pests.

Journal Article↗
PubMed1986

A comparison of the induced polypeptides and RNAs of three orbiviruses isolated from ticks (Ixodes uriae) collected in seabird colonies on the Isle of May, Scotland.

Three orbiviruses of the Kemerovo serogroup, isolated from ticks from two sites on the Isle of May in Scotland, were plaque purified and the proteins and RNA induced in Vero cells compared. Two viruses (Mill Door/79 and Mill Door/81) from the same site differed in the migration of at least 7 segments of dsRNA. The third virus (North Clett/81) was clearly distinguished from Mill Door/79 and Mill Door/81 viruses in the migration of 6 dsRNA segments. Analysis of virus-induced polypeptides demonstrated minor molecular weight differences, but partial proteolysis failed to show significant variations in the proteins. Precipitation of radiolabelled virus-induced proteins by hyperimmune ascitic fluids did not distinguish between the isolates.

Animals↗
PubMed1986

Characterisation of orbiviruses of the Kemerovo serogroup: comparison of protein and RNA profiles.

Nine viruses of the Kemerovo serogroup (orbivirus genus; family, Reoviridae) isolated from seabird ticks (Ixodes uriae and Ornithodoros maritimus) from eight different geographical locations (four from Scotland, two from Morocco, one from Eire, one from England, one from the Faeroes Islands) were examined. All nine viruses produced unique RNA electropherotypes but showed a 2 : 4 : 3 : 1 grouping of the ten double-stranded RNA segments. The virus-induced proteins were labelled with 35S-methionine in a Vero cell line. Seven of the viruses isolated from Ixodes uriae produced similar profiles of nine major polypeptides. The remaining two viruses, isolated from Ornithodoros maritimus from Morocco, produced profiles in which only five major viral proteins could be readily detected, four of which had similar molecular weights to proteins apparent in the other isolates.

Animals↗
PubMed1985

The proteins expressed in vivo and in vitro by an orbivirus of the Kemerovo serogroup isolated from Ixodes uriae ticks from St. Abb's Head, Scotland.

The genome of FT363 virus, a member of the Kemerovo serogroup of orbiviruses, was shown to consist of 10 segments of dsRNA. Examination of the intracellular proteins expressed by this virus in Vero cells showed the presence of 9 major and several minor products. Similar results were obtained by translation of the virus dsRNA in a rabbit reticulocyte lysate. Comparison of in vivo and in vitro products by immunoprecipitation using polyclonal FT363 ascitic fluid showed a difference in the numbers of polypeptides precipitated. The significance of these results is discussed.

Animals↗
PubMed1985

Replication and polypeptide synthesis of Mill Door/79, an orbivirus isolated from ticks from a seabird colony in Scotland.

The replication and polypeptide synthesis of orbivirus isolate Mill Door/79, a member of the Kemerovo serogroup, were studied. In Vero cells cell-associated virus exceeded cell-free virus by about 2 log10 PFU/ml. Attempts to purify the virus resulted in the demonstration of five polypeptides. Thirteen virus-induced polypeptides and 10 segments of double-stranded RNA were identified in infected cells. Partial proteolysis demonstrated homology between some polypeptides.

Animals↗
PubMed1985

Gene assignments of a cytoplasmic polyhedrosis virus (type 2) from Nymphalis io.

The 10 double stranded (ds) RNAs from cytoplasmic polyhedrosis virus (type 2) from Nymphalis io have been separated by polyacrylamide gel electrophoresis and fractionated into separate genes. Separate and total dsRNA were denatured using methyl mercury, and translated in a rabbit reticulocyte lysate. Translation of unfractionated RNA generated proteins that comigrated with virion and polyhedral structural proteins and six major non-structural proteins. Translation of individual RNAs, demonstrated that the virion structural proteins were coded for by the largest RNAs and the polyhedral protein by the smallest genome segment. RNAs 7 and 8 each produced two major non-structural proteins and no primary product was found for RNA 3. Reaction of the translation products with antiserum raised against purified virions immunoprecipitated putative structural proteins (and some "non-structural"). Antiserum against the polyhedral protein immunoprecipitated "non-structural" proteins and the polyhedral protein, but not virus particle structural proteins.

Genes, Viral↗
PubMed1984

Characterization of a Small RNA-Containing Virus in Field-Collected Larvae of the Tussock Moth, Lymantria ninayi, from Papua New Guinea.

Field-collected larvae of the tussock moth, Lymantria ninayi, a major pest of exotic pines in Papua New Guinea, were found to contain a small RNA-containing virus with a diameter of 29 nm and a buoyant density of 1.32 g/ml. The RNA was single stranded, had a molecular weight of 2.8 x 10, and was polyadenylated. Virion RNA stimulated an in vitro translation system, and high-molecular-weight proteins were produced. Purified virions contained four structural proteins with molecular weights of 43,000, 38,000, 33,000, and 32,000. The virus reacted positively with antisera raised against a strain of Drosophila C virus. The properties of this virus indicate that it should be placed in the family Picornaviridae.

Journal Article↗
PubMed1983

Cell-free translation of Drosophila C virus RNA: identification of a virus protease activity involved in capsid protein synthesis and further studies on in vitro processing of cricket paralysis virus specified proteins.

Drosophila C virus RNA acted as mRNA in rabbit reticulocyte lysates and directed the synthesis of at least one capsid protein and a number of higher molecular weight proteins. Kinetic analysis by pulse-chase experiments showed that a number of high molecular weight products acted as precursors to the capsid protein(s). Various dilution experiments were performed which showed that the virus specified a protease activity essential for the correct processing of precursors to give the capsid protein(s). A similar result was obtained with Cricket paralysis virus, and mixing experiments showed that the protease activity specified by one virus could perform some of the cleavages resulting in the production of the capsid proteins of the other virus. Some of the cleavages involving the highest molecular weight precursors could not be performed by the protease activity of the other virus. We could find no evidence for intramolecular cleavage of the capsid precursors of either of the viruses.

Animals↗
PubMed1983

The gene organisation of a small RNA-containing insect virus: comparison with that of mammalian picornaviruses.

The coding regions of an insect virus, cricket paralysis virus, have been mapped using pactamycin. The results suggest that the genome of this virus functions as a polycistronic mRNA, the structural proteins being encoded by the 5' end of the RNA in an order similar to those of mammalian picornaviruses. High-molecular-weight proteins of unknown function map at the 3' end of the genome.

Animals↗
PubMed1983

Translational inhibition of heat-shock induced gene expression in picornavirus-infected Drosophila melanogaster cells.

Picornavirus infection of Drosophila melanogaster cells inhibited the appearance of heat-shock induced proteins. Examination of intracellular mRNAs revealed that those coding for heat shock proteins were present in a translationally competent form in infected cells. Inhibition of induced gene expression in infected Drosophila cells therefore involves, but is not necessarily solely mediated by, effects at the level of translation.

Animals↗