Biomedical subjects

N Filipović

Publications and source records attributed to N Filipović.

At least 19 recordsLinked to original sources

PubMed2005

[Changes in concentrations of sera proteins in war wound].

UNLABELLED: During the general reaction to trauma, substantional changes in protein composition of sera occure. The aim of the prospective study was to investigate net change in total protein and albumin concentrations, as well as albumin/globulin ratio in sera of war casualties during the first 14 posttraumatic days, and to establish the correlation between these changes and severity of trauma. Subjects were 79 war casualties. CONTROLS: 33 blood donors. METHODS: Injury severity was determined according to ISS and blood samples were collected 12 hours after trauma, then on the 1st, 2nd, 5th and 14th posttraumatic day. In war casualties values of total protein and albumin concentrations and albumin/globulin ratio were significantly decreased. Minimal concentrations were measured on 2nd posttraumatic day (589.04 g/l for total proteins, p; 36.66.21 g/l for albumins, p) or on 5th day (0.860.2 for albumin/globulin ratio, p). CONCLUSIONS: During the acute-phase response to trauma, significant changes in concentration of total proteins, albumins and albumin/globulin ratio occure in sera of war casualties. These changes are the most promminent during the first 5 days, with tendency for normalization after that. Intensity of these changes depends of the severity of trauma.

Adult↗

PubMed2001

[Total intravenous anesthesia using remifentanil and propofol with midazolam co-induction in laparoscopic surgery of the gallbladder].

We investigated effects of total intravenous anesthesia (TIVA) with propofol and remifentanil (in two parallel continuous infusions), on 28 ASA I-II patients undergoing laparoscopic cholecystectomy. All patients received midazolame (0.05 mg/kg b.w.), and 90 sec thereafter, remifentanil (0.5 g/kg b.w.). Computer controlled intravenous infusion of propofol started at dose of 6 mg/kg/h (by Graseby 3400 Syringe Pump). Muscle relaxation was achieved by rocuronium (0.6 mg/kg b.w.). After endotracheal intubation, rate of propofol was decreased on 3 mg/kg/h and started with another infusion of remifentanil (0.5 ug/kg/min). Before (T0) and after induction (T1), after start of surgery (T2), and at the end of surgery (T3), we evaluated: systolic, diastolic, and medial arterial blood pressure (SAP, DAP, MAP), heart rate (HR), peripheral saturation of O2 (O2Sat), and capnometry (ETCO2), by Datex-Engstrome AS/3 Monitore. It was followed side effects of anaesthesia, early and complete recovery rate, and frequency of nausea and vomiting in postoperative period. Results showed haemodynamic stability of patients after induction in anaesthesia (defined as decreasing of MAP 20%, compared with preinduction values). During investigation (T0-T3), results of 0.2Sat and ETCO2 were excellent (0.60 +/- 2 and 5.1 +/- 2.4 min). There wasonly one case of postoperative nausea and vomiting, and no significant side effects of anaesthesia. TIVA remifentanil-propofol and co-induction with midazolame makes possible haemodynamic stability of patients after induction in anaesthesia, good oxygenation during surgery, fast early and complete recovery, and avoiding of side effects of anaesthesia and postoperative nausea and vomiting. We concluded that it is a good choice of anaesthesia for laparoscopic cholecystectomy.

Adult↗

PubMed1991

[Urinary N-acetyl-beta-D-glucosaminidase in workers exposed to the effects of mercury].

Mercury concentration in urine and urinary activity of N-acetyl-beta-D-glucosaminidase were determined in a group of 100 workers from an electrolysis workshop. The enzyme activities measured were twice as high as those of 100 control subjects. The difference was statistically significant. There was no correlation between urinary activity of N-acetyl-beta-D-glucosaminidase and mercury concentration in urine. The applicability of this biochemical-toxicological test in occupational health practice is discussed.

Acetylglucosaminidase↗

PubMed1991

[N-acetyl-beta-D-glucosaminidase as an early marker and indicator of the extent of kidney and liver lesions in endotoxemia and endotoxic shock].

The aim of the study was to answer the question whether the serum level and the urine level of N-acetyl-beta-D-glucosaminidase (NAG) could be the indicator of liver and kidney lesions, and the indicator of the stage of the lesions, in cases of endotoxemia and endotoxin shock. The authors performed the experiment using 60 rabbits as experimental animals. The animals were divided into six groups (10 rabbits in each group); control animals received an equal volume (1.0 ml/kg of body weight) of physiological saline solution. Endotoxin (Lipopolysaccharide E. coli 055 B-5) in doses of 20, 40, 60, 80, and 100 micrograms/kg of body weight were administered in a single intravenous injection to each animal of I-V experimental groups. Using endotoxin in that dose of application, the authors provoked different stages of endotoxemia on the rabbits in the first four experimental groups, and the endotoxin shock on the rabbits of the fifth experimental group. After 18 hours, the rabbits were decapitated, and the levels of NAG in serum, urine, kidney and liver tissues determined. The results showed that the serum increase of NAG activity is caused not only by the lesions of kidney parenchyma, but by the lesions of liver parenchyma too, in the cases of endotoxemia and endotoxin-produced shock; the increase is statistically significant. Urine increase of NAG activity is significant even in cases in which the authors administered very low doses of endotoxin (20 micrograms/kg of body weight), so it can be said that urine NAG activity is a very good indicator of early kidney parenchyma lesions. But, the urine increase of NAG activity is (absolutely) in correlation with the dose of administered endotoxin, so it can be said that urine activity of NAG is the indicator of stage of kidney lesions in cases of endotoxemia and endotoxin-provoked shock.

Acetylglucosaminidase↗

PubMed1989

[Angiotensin converting enzyme activity in the blood of dogs with posthemorrhagic hypotension].

In 1984 and 1985 a questionnaire was applied to 5611 railway transport workers in Croatia. Out of them, those satisfying the criteria of the Geneva Convention (1980) of the International Working Group for the Early Detection of Colorectal Carcinoma (3379) were supplied with the Hemdetect test. Of these 3379 workers, 2428 (71.8%) returned the "consumed" test after three days: in 114 of them the Hemdetect test was positive. Since in this way 5 patients with colorectal carcinoma (4.85%), 17 with polyps (16.5%), and 3 with polyposis (2.92%) were detected, it is maintained that the Hemdetect test is very useful, in fact unavoidable in attempts at the secondary prevention of colorectal carcinoma and the lesions proceeding it. Although the 34.95% of so-called false positive results obtained by the Hemdetect test diminish its significance because of a reduced specificity, it should not be excluded from practical work on the secondary prevention of colorectal carcinoma--it should in fact be put ahead of other tests, because it is simple, easily applied, readily acceptable by examinees, and less costly than the use of immunochemical methods.

Animals↗

PubMed1989

[Angiotensin converting enzyme in the blood and lungs in experimental diabetes].

The activity of angiotensin converting enzyme has been examined in serum and lungs of Wistar male rats with streptozotocin induced diabetes. The diabetes has been induced by single i.v. dose of streptozotocin (75 mg/kg body weight). The activity of angiotensin converting enzyme in serum and lungs has been measured ten days after application of drug by spectrophotometrical method using synthetic substrate hippuryl-1-histidyl-1-leucine. The results show that streptozotocin induced diabetes in rats cause significant increase in angiotensin converting enzyme activity in serum and lungs.

Animals↗

PubMed1988