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N Funayama

Publications and source records attributed to N Funayama.

29 records · Page 2Linked to original sources
PubMed1990

Physical and genetic analyses of IncI2 plasmid R721: evidence for the presence of shufflon.

A physical map of the 75.1-kb IncI2 plasmid R721 was constructed by using 15 restriction enzymes, and the regions of several genetic determinants including the origins of replication and of conjugal DNA transfer were located on the physical map. It was found that R721 bears a DNA region which undergoes DNA rearrangement similar to the shufflon of R64.

Conjugation, Genetic↗
PubMed1990

Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer.

To locate the transfer region of the 122-kiloase plasmid R64drd-11 belonging to incompatibility group I1, a series of deletion derivatives was constructed by in vitro recombinant DNA techniques followed by double homologous recombination in vivo. A plasmid designated pKK609 and bearing a 56.7-kilobase R64 sequence was the smallest transferable plasmid. A plasmid designated pKK610 and no longer possessing the 44-base-pair sequence of the R64 transfer system is located at one end. The other end of the R64 transfer region comprises a DNA segment of about 19 kilobases responsible for pilus formation. Shufflon, DNA with a novel rearrangement in R64, was found to be involved in pilus formation.

Chromosome Deletion↗
PubMed1990

Regeneration of endothelial cells after balloon denudation of the rabbit carotid artery and changes in responsiveness.

The present experiments were carried out to determine the regrowth of endothelial cells (EC) after balloon denudation of the rabbit carotid artery and the changes in responsiveness of the artery with regenerated EC. Scanning electron microscopic findings revealed that 28.8% of the luminal surface was covered with regenerated EC at week 1. The regrowth of EC proceeded progressively, and a full lining was achieved at week 6. Regenerated EC were morphologically different from native ones; they were elongated (weeks 1 and 2) and irregularly oriented (weeks 4 and 6), and their numbers had significantly increased. Light microscopy revealed the intimal thickening and proliferation of smooth muscle cells. No accumulation of lipids in the vascular wall could be detected at any observation time. The experiments in an organ bath demonstrated that the altered appearance of EC was accompanied by depressed endothelium-dependent relaxations to acetylcholine, ADP and A23187. However, sodium nitroprusside-induced relaxation and contractile responses to noradrenaline, serotonin and histamine remained unchanged in the normal and denuded preparations, indicating that the dysfunction of the endothelium occurs at a time when the ability of the underlying vascular smooth muscle to relax or contract was unchanged. In addition, it is suggested that the impairment of the endothelium-dependent relaxation may be partly due to impairment of the synthesis and/or release of endothelium-derived relaxing factor(s) in EC.

Acetylcholine↗
PubMed1989

Histological evidence of left ventricular involvement in arrhythmogenic right ventricular dysplasia.

A 40-year-old female with arrhythmogenic right ventricular dysplasia (ARVD) demonstrated a reduced motion of the left ventricular (LV) apex. Specimens of LV free wall, obtained by endomyocardial biopsy, histologically revealed prominent interstitial fibrosis with sparse distribution of myocytes. The myocytes were hypertrophic and disrupted with loss of myofibrils. This is a case of ARVD, where LV involvement was histologically verified.

Adipose Tissue↗
PubMedDate not supplied

[A case of acute myocardial infarction complicated with left ventricular pseudoaneurysm and ventricular septal rupture].

An 87-year-old woman was admitted to another hospital with acute inferior myocardial infarction on May 31, 1991. On the 6th hospital day she suddenly developed transient complete A-V block and ventricular tachycardia. She was transferred to our hospital for the treatment of intractable heart failure on the 18th hospital day. Two-dimensional echocardiography showed a saccular chamber with a narrow-necked connection to the left ventricle. Color Doppler echocardiography showed bidirectional blood flow between the left ventricle and saccular chamber during systole and diastole. There was 35% left to right shunt in the ventricular level on right heart catheterization. Acute myocardial infarction complicated with left ventricular pseudoaneurysm and ventricular septal perforation was diagnosed. She died on the 26th hospital day without aggressive medical treatment. Autopsy demonstrated the pseudoaneurysm in the posterior wall of the left ventricle and the connection to the right ventricle. The so-called double rupture could be diagnosed before death.

Aged↗