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N G Heatley

Publications and source records attributed to N G Heatley.

18 recordsLinked to original sources
PubMed1977

Production of antibody to staphylococcal delta-haemolysin in the rabbit.

Gamma-globulin and IgG from the sera of rabbits immunized with either "insoluble" or "solvent-transferred" staphylococcal delta-haemolysin precipitated with, and neutralized both forms of lysin. Similar preparations from the same rabbits before immunization did not; nor did those from rabbits made hyperimmune to other antigens. In these tests "insoluble" and "solvent-transferred" lysin could not be distinguished antigenically, nor could "insoluble" lysin from two different strains of staphylococci, each grown on two different media. An appendix presents further evidence for antigenicity of delta-lysin, based on affinity chromatography.

Animals↗
PubMed1976

Unusual behaviour of a protein: reversible quantitative precipitation of staphylococcal delta-haemolysin by low concentrations of some organic solvents.

Staphylococcal delta-haemolysin is precipitated from watery solutions by low concentrations of chloroform. The effect on this precipitation of pH, kind and concentration of salts, and concentration of, and time of exposure to, chloroform have been investigated. Some but not all of the other solvents tested cause a similar precipitation. The haemolytic activity is not destroyed and under appropriate conditions precipitation is quantitative. The dry lysin will take up nearly double its weight of chloroform when exposed to the vapour. Some is bound strongly.

Ammonium Sulfate↗