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N G Menzianova

Publications and source records attributed to N G Menzianova.

5 recordsLinked to original sources
PubMed1993

[Population kinetics of circulating monocytes in health and in patients with ischemic heart disease].

Population kinetics of monocytes (MC) was studied to prove their participation in atherogenesis. Increased number of cells containing lipids (CCL) in patients with severe stable angina pectoris is demonstrated (42.5 versus 7.4% in healthy persons). This is followed by MC esterase activity enhancement. In patients with angina of effort new types of MC appear (with high peroxidase and low esterase activity) and there is a drastic increase of CCL (more than 56%). The correlation between the number of CCL and indices of blood lipid metabolism in anginal patients is lacking. The results obtained may serve as a criterion for evaluating the degree and exacerbation of coronary atherosclerosis in humans.

Adult↗
PubMedDate not supplied

[The genetic differences in the microsomal activity of rats].

The lipid composition and synthesis rate in liver microsomes of linear and hybrid rats are investigated. It is found that microsomes of hybrid rats had less amount and synthesis rate of phosphatidylcholine and phosphatidylethanolamine as well as nonesterified fatty acids. Linear rats differed from each other in phosphatidylinositol exchange rate. These genetic peculiarities of the microsomal lipid composition and synthesis rate can explain the experimentally determined absence of correlation between glucose-6-phosphatase activity and lipid maintenance in membranes of different genetic forms.

Animals↗
PubMedDate not supplied

[An improved method for isolating blood monocytes].

The authors have improved the method for the gradient isolation of monocytes from human whole blood: an expensive reagent, percoll, is replaced by verograffin, an x-ray contrast agent. Monocytes were isolated by this method from the blood of 8 normal subjects. The isolated monocytes were identified and their population composition studied electronmicroscopically, cytochemically, and immunocytochemically. The monocyte population of healthy donors was found morphologically and cytochemically heterogeneous, consisting of 4 types of cells, forming on the monocytogram stable (in their per cent shares) classes. The improved method for monocyte isolation and the results of morphometric analysis may be used to assess the system of human mononuclear phagocytes in various diseases.

Adult↗
PubMedDate not supplied

[Isolation of two fractions of hepatocytes and characterization of their lipid composition].

Two fractions of hepatocytes were isolated from the rat liver by nonfermentative method. These fractions were different for mechanical stability to the action of perfusion factors. It has been shown that cells of these fractions were differently separated in the linear density gradient of sucrose. It was connected with structural and functional heterogeneity of hepatocytes in the liver. Results obtained allow us to confirm that hepatocytes in the liver form at least two classes of cells with different lipid content. The plasmatic membranes of hepatocytes with high content of lipids were inclined to damage in the process of liver perfusion as compared to cells with low lipid content.

Animals↗