Biomedical subjects

N Galić

Publications and source records attributed to N Galić.

8 recordsLinked to original sources

PubMed2006

Genotoxicity evaluation of five different dentin bonding agents by chromosomal aberration analysis.

Dentin bonding agents became unavoidable in today's aesthetic restorative dentistry. Nevertheless, more and more evidences on their possible cytotoxicity and/or genotoxicity emerge. Still, only limited number of studies has been published on that issue. In our work we evaluated possible genotoxicity of five different adhesives: Adper Single Bond, Adper Single Bond 2 with nanofiller, Excite, OptiBond Solo Plus and Prompt L-pop. Genotoxicity assessment was carried out on human lymphocytes in vitro, using chromosomal aberration analysis. Polymerized adhesives were tested at three different dilutions of the 0.5 g mL(-1) eluate stock (2.5 x 1:10(6), 1:10(6) and 1:10(5)) after 1 h, 24 h and 5 days of elution. Slight but significant increase in the number of chromatid breaks was observed after 24-h elution period, for adhesives Adper Single Bond 2, Excite, and OptiBond Solo Plus at dilutions of 1:10(6) and 1:10(5), and for other two only at dilution of 1:10(5). First three adhesives also appeared to be slightly genotoxic after 1 h of elution but only at 1:10(5). As a bonding agent remains in close contact with living dental tissue over a long period of time, information on their possible genotoxicity and carcinogenicity should be more clearly clarified in the near future.

Case-Control Studies↗

PubMed2002

Evaluation of sporadic cases of odontogenic keratocysts in multicentric study.

The aim of this retrospective study was to report frequency of sporadic odontogenic keratocysts (sOKC) according to the age and gender, as well as location (mandible, maxilla, soft tissues, and maxillary sinus). Four hundred and twenty nine sOKC confirmed pathohistologically in a period from 1965-1998 were included in this study. The average age of patients with sOKC was 43.11 (age range 10-91), in males 42.06 and in females 44.72 years. More frequently sOKC were found in males (60.61%) in comparison to the females (39.39%). Therefore, ratio between males and females was 1.5:1. Diagnosis of sOKC is usually established in patients aged 21-30 (18.88%), in males usually aged between 21-30 (23.46%), and in females aged between 11-20 (18.93%). sOKC are more frequent in males according to the age groups, except between age 61-70 where sOKC were more frequent in females. Most frequently, sOKC occurred in the mandible 70.16%, 12.35% of sOKC were found in the maxilla, 12.82% in soft tissues and 4.66% in the maxillary sinuses.

Adolescent↗

PubMed2001

Elimination of mercury from amalgam in rats.

The aim of this study was to measure the urinary mercury excretion in rats exposed to amalgam over a two months period. Animals were either exposed to mercury from 4 dental amalgams or fed the diet containing powdered amalgams. The results showed significantly higher mercury amount in urine of both exposed groups than in control. Even two months after the amalgam had been placed in rats teeth, the amount of mercury in the urine remained 4-5 times higher than in control, and 4 times higher than in rats exposed to diet containing powdered amalgam. The elevated urinary Hg amount was accompanied by an increased level of total protein in urine. In the same exposure period the excretion of total protein in urine of rats with amalgam fillings was 2 times higher than in control and 1.5 times higher than in rats exposed to amalgam through diet. Concentrations of mercury in the sera of all groups were below the detection limit of the method. The results show that amount of mercury and protein in the urine of rats were related to the mercury release from dental malgam.

Animals↗

PubMed1999

Dental amalgam mercury exposure in rats.

The aim of this study was to measure the distribution of mercury, in tissues of rats exposed to amalgam over a two months period. Possible interaction of mercury with copper and zinc in organs was also evaluated. Rats were either exposed to mercury from 4 dental amalgams, or fed the diet containing powdered amalgam during two months. Mercury was measured in the kidney, liver and brain, copper in kidney and brain and zinc in kidney. The results showed significantly higher concentrations of mercury in the kidneys and the brains of rats in both exposed groups compared to control. Even after two months of exposure to mercury brain mercury concentration in rats with amalgam fillings was 8 times higher than in the control and 2 times higher than in rats exposed to amalgam supplemented diet. The highest mercury concentration in the latter group was found in the kidneys and it was 5 times higher than in the control group. We found no significant differences between mercury levels in exposed and control rat's liver. Exposure to mercury from dental amalgams did not alter the concentrations of copper and zinc in the tissues. Histopathological analyses of rats tissues did not show any pathological changes. These results support previously proposed nose-brain transport of mercury released from dental amalgam fillings.

Animals↗

PubMed1999

Alterations in optic canal following resection of nerve in rats.

The authors have studied relations between bony optic canal and nerve elements resulting from lesion in the optic nerve. Experimental animals were divided in two groups: young adults and old adult rats; the non-operated sides of rat skulls were used as a control group. At the same time in control samples the changes in osseous openings resulting from ageing were studied. Four months after surgery the animals were sacrificed, their skulls macerated and morphometric measurements taken by photometric and planimetric techniques. The obtained results show no significant difference in size of bony openings between the operated and non-operated sides. A significant difference has been found in the size of optic canal between the young and the old rats, regardless of the operated side. The authors have thus come to the conclusion that the size of bony canals in adult animals depends on their age in the first place. Narrowing of bony canaliculi, observed in the course of ageing, is most probably caused by primary osseous apposition, while atrophy of nerve elements may be considered as a secondary event.

Aging↗

PubMed1998

Trichomonas tenax proteolytic activity.

In this study, proteolytic activity of Trichomonas tenax collected directly from patient's dentobacterial plaque was examined. Electrophoretic method involving polyacrylamide gels (Commassie Brilliant Blue R-250) and electrophoretic method involving gelatin-containing polyacrylamide gels, have been used to analyse Trichomonas tenax proteolytic activity. The most obvious and the fastest activities were obtained when gels were incubated in pH 4.6; followed by results of incubating in pH 5.6; while in pH 2.8 activity was less effective but still obvious. Proteolytic activities were the most effective in area of protein MW 36 kDa. Different activities of enzymes depending on pH of incubated media indicate the presence of different endopeptidases in cell lysates of protozoon Trichomonas tenax from dentobacterial plaque.

Animals↗

PubMed1998

The influence of dental amalgam on the blood count.

The purpose of this research was to determine the influence of dental amalgam on the blood count of the holder of amalgam fillings. The experiments were performed on 30 Wistar rats. One group of rats received 8 mg of fragmented dental amalgam daily incorporated in their food during 60 days (group S2) and group S3 received amalgam fillings in their anterior teeth, which remained for 60 days. Both experiment groups (S2 and S3) were compared to one another as well as to control group (S1). From all animals of all experiment groups blood samples were drawn during the experiment in particular periods and complete blood count and differential blood count was performed. The findings of particular indicators of the complete blood count and differential blood count of all experiment rats groups S1, S2 and S3 do not deviate from the physiological values regarding age and sex of the observed animals.

Animals↗

PubMed1998

Comparison of dental maturity between two ethnic groups.

Dental maturity is one of the indicators of the craniofacial region growth and development. The aim of the study was to identify and compare dental growth and development in two ethnic groups. The material consisted of 480 orthopantomograms; 240 selected from orthodontic and dental patients from Zagreb (Croatia) and Damascus (Syria) each. Each ethnic group included 120 boys and 120 girls, while the radiographs were classified into 6 groups of 20 each, according to sex and chronologic age (8-13 years). Dental maturity was evaluated by the method of Nolla. The results were statistically analyzed and presented in tables. The subjects from Damascus showed a delay in dental maturity by 1 (girls) to 2 (boys) years compared to the homologous group from Zagreb. The reference values determined in the study may serve as a diagnostic, therapeutic and prognostic tool in the orthodontics and pedodontics of the respective cities.

Child↗