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Biomedical subjects

N Gay

Publications and source records attributed to N Gay.

51 records · Page 3Linked to original sources

Possible increase of dopamine-beta-hydroxylase activity in the locus ceruleus of paranoid schizophrenic patients: a preliminary post-mortem study.

Five schizophrenic brains and seven control brains were compared for phenylethanolamine-N-methyl-transferase and dopamine-beta-hydroxylase activities. The two groups were comparable in age, sex, and post-mortem delay before autopsy. A radioenzymatic assay showed a statistically significant increase of dopamine-beta-hydroxylase activity in the rostral part of the locus ceruleus in the schizophrenic brains.

Adult↗

Long-lasting insomnia induced by preoptic neuron lesions and its transient reversal by muscimol injection into the posterior hypothalamus in the cat.

In order to analyse the role of the anterior hypothalamus in the regulation of the sleep-waking cycle we made bilateral neuronal lesions at different levels of the anterior hypothalamus in cats, by means of microinjections of a cell-specific neurotoxin:ibotenic acid. These lesions resulted in severe insomnia in eight cats. This insomnia was characterized by a large decrease or even disappearance of paradoxical sleep and deep slow wave sleep and, to a lesser extent, by a decrease of light slow wave sleep, for 2-3 weeks. In the other five animals, we observed a large reduction of deep slow wave sleep (0-40% of control level), but a less intensive decrease of time spent in paradoxical sleep (50-75% of control level) and no marked effect on light slow wave sleep. During the first 3-6 postoperative days we also noticed hyperthermia in all cats; thereafter, the animals presented only a slight increase in brain temperature which did not appear to trigger the sleep impairment. Histological analysis of the different lesions revealed that the insomnia could be attributed to neuronal cell body destruction in the mediobasal part of the anterior hypothalamus covering; the medial preoptic area and a narrow portion of the lateral preoptic area as well as a restricted part of the anterior hypothalamic nucleus. In order to investigate the putative role of the posterior hypothalamic structures in the mechanism of insomnia after lesion of the mediobasal preoptic area neurons we injected an agonist of GABA into the ventrolateral part of the posterior hypothalamus to locally depress the neuronal activity. The bilateral intracerebral microinjection of muscimol (0.5-5 micrograms) induced a transient intensive hypersomnia (slow wave sleep and paradoxical sleep). These findings indicate that neuronal cell loss in the mediobasal preoptic area induced a long lasting insomnia. Thus, it may be hypothesized that the integrity of this structure is necessary for sleep appearance. Finally, our data are in keeping with an intrahypothalamic regulation of the sleep-waking cycle.

Animals↗

[Comparative efficacy of antibiotic prophylaxis using cefoxitin in one or in three doses in cesarean section].

To compare the effectiveness of three doses and of a single dose of cefoxitin, a second-generation cephalosporin, in preventing infection after nonelective cesarean section, we carried out a prospective study in 255 women who underwent the procedure between March 1983 and February 1985. The rate of postoperative infection was 7% in the group that received three doses and 8% in the one-dose group. The sensitivity and specificity of perioperative cultures were low. The rate of asymptomatic bacteriuria was high, at 20%, but few symptomatic urinary tract infections were found. Routine antibiotic prophylaxis with a single dose is suggested for all nonelective cesarean sections.

Adult↗

Effects of endophyte-infected fescue on concentrations of prolactin in blood sera and the anterior pituitary and concentrations of dopamine and dopamine metabolites in brains of steers.

An experiment was conducted to determine if the decrease in circulating concentrations of prolactin in cattle consuming endophyte (Acremonium coenophialum) -infected tall fescue (Festuca arundinacea) was associated with changes in prolactin concentrations in the anterior pituitary and concentrations of dopamine (DA) and its metabolites 3,4-dihydroxyphenyl-acetic acid (DOPAC) and homovanillic acid (HVA) in the stalk median eminence (SME), preoptic area (POA) and hypothalamus (HP). Six crossbred steers that grazed high-endophyte (greater than 90% infected) fescue and four steers that grazed low-endophyte (less than 1% infected) fescue from April to September were slaughtered. Brains and pituitaries were removed and dissected. Extracts from neural tissue were analyzed for DA, DOPAC and HVA using high performance liquid chromatography/electrochemical detection. Pituitary extracts and sera from blood samples taken 5 d prior to slaughter were subjected to prolactin radioimmunoassay. Consumption of high-endophyte fescue was associated with decreased concentrations of prolactin in serum (P less than .01) and in the anterior pituitary (P = .08), decreased (P less than .05) concentrations of DA in the SME and decreased (P less than .01) concentrations of HVA in the POA and HP, but it did not influence levels of DOPAC. These results suggest that endophyte toxins may reduce prolactin synthesis and release and may alter activity of dopaminergic neurons.

3,4-Dihydroxyphenylacetic Acid↗

Catecholamine synthesizing enzyme activity in brainstem areas from victims of sudden infant death syndrome.

In order to investigate whether central catecholaminergic neurons are altered in sudden infant death syndrome (SIDS), the activities of dopamine-beta-hydroxylase (DBH) and phenylethanolamine-N-methyltransferase (PNMT) were determined in nine brainstem areas from 19 victims of totally unexplained SIDS (tSIDS), 9 victims of partially unexplained SIDS (pSIDS) and 8 control infants. The distinction between tSIDS and pSIDS was made according to the results of the autopsy. They were totally negative for the tSIDS victims, while minor pathological alterations were found in pSIDS victims. In both tSIDS and pSIDS victims, the PNMT activity was significantly decreased in the medullary C2m and C21 areas and in the nucleus medullae oblongatae centralis. In the C21 area only DBH activity was decreased. Further, in the nucleus ambiguus PNMT activity was decreased in tSIDS, while DBH was lowered in pSIDS victims. No significant modifications were found in the areas from the dorso-lateral pons (locus coeruleus, Kolliker Fuse and parabrachialis nuclei). Thus, in the two groups of SIDS victims the capacity to synthesize adrenaline was decreased in some medullary areas. It is considered that these biochemical alterations are related to the abnormal central respiratory and arousal control, which is thought to be present in SIDS.

Brain Stem↗

Growth and metabolism of growing beef calves fed tall fescue haylage supplemented with protein and(or) energy.

Endophyte (Acremonium coenophialum)-infected Kentucky 31 tall fescue was fertilized in mid-August, stockpiled, harvested November 4 to 6 and stored in a concrete stave silo. Ninety-six growing calves (189 kg) were assigned to the following treatments (24 calves/treatment): 1) corn silage (CS) plus .4 kg/d of soybean meal (SBM; 2) fescue haylage plus .4 kg/d of SBM; 3) fescue haylage plus 1.6 kg/d of corn and 4) fescue haylage plus 1.6 kg/d of corn and .4 kg/d of SBM. Daily gains and dry matter (DM) intakes during the 91-d trial were .52, 4.58; .51, 5.22; .59, 6.06; and .63, 6.18 kg for treatments 1 through 4, respectively. Daily gains of calves fed corn silage plus SBM and fescue haylage plus SBM were not different (P greater than .05). However, a difference (P less than .05) existed between treatments 1 and 2 vs 3 and 4. Feed conversion was improved (P less than .05) in calves fed corn silage. Calves in a metabolism trial were fed either 1) 6.2 kg November-ensiled fescue haylage or 2) 6.2 kg November-ensiled fescue haylage plus 1.6 kg/d of corn. Digestibility of DM, N-free extract (NFE) and TDN did not differ (P greater than .05) between treatments. Ether extract digestibility was greater (P less than .05) for the added corn diet, while that of CP was greater (P less than .05) for the fescue haylage diet. Nitrogen retained was higher (P less than .05) for calves fed added corn. A follow-up trial with 96 growing calves (190 kg) compared September- and November-harvested fescue haylages supplemented with either 1.3 or 2.6 kg corn/d.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗

Effect of soybean lipid on growth and ruminal nitrogen metabolism in cattle fed soybean meal or ground whole soybeans.

Three experiments were conducted to determine the effect of soybean lipid on ruminal proteolysis of soybean meal (SBM) and ground whole soybeans (GSB). Experiment 1 was a 92-d growth experiment using 120 calves (206 kg) allotted to 12 pens of 10 calves each. Three replicate pens were assigned to each of the treatment supplements: low SBM (LSBM), low GSB (LGSB), high SBM (HSBM) and high GSB (HGSB). Calves received ad libitum amounts of corn silage top-dressed with the respective supplement (.81 kg/head). High protein supplements produced greater (P less than .05) gains than low protein supplements, with HSBM calves gaining faster (P less than .05) than HGSB calves and LSBM and LGSB calves having similar (P greater than .10) gains. In Exp. 2, 15 ruminally cannulated Angus X Hereford heifers (380 kg) fed corn silage were used to determine ammonia-N release from the treatment supplements: ground corn (control), GSB, SBM and SBM coated with soybean oil (SBMO). Heifers fed the control supplement had lower (P less than .05) ruminal NH3-N concentrations than those consuming soybean protein. Ruminal NH3-N concentrations were similar (P greater than .10) for GSB and SBM; whereas, SBMO had lower (P less than .10) concentrations than SBM through 3 h. In Exp. 3, two ruminally cannulated Angus X Jersey steers (250 kg) were used to determine in situ disappearance of SBM, GSB and SBMO. Total and feed N disappearances were greater (P less than .001) for GSB than SBM or SBMO.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗

Labile magnesium reserves in beef cows subjected to different prepasture supplementation regimens.

Thirty pregnant beef cows were utilized to determine the effects of winter Mg supplementation regimen on blood minerals after cows were turned to a spring tetanigenic tall fescue pasture. The winter Mg treatments were (1) tall fescue hay with free access to a Mg-deficient salt-mineral mix, (2) 6.4 kg of corn silage dry matter plus 114 g MgO/d and (3) tall fescue hay with free access to a salt-mineral mix containing 40% MgO from January 1 to February 15. All cows were then placed on the same tetanigenic pasture from February 15 to April 17 with free access to a Mg-deficient salt-mineral supplement. Forage Mg remained below .2% during the experiment. Forage Ca, P and Al changed throughout the spring, attaining maximum values of .35%, .46% and 415 ppm, respectively. Forage N and K also increased throughout the spring, reaching values of 3.5 and 3.8%, respectively, at the April 3 sampling. The forage K:(Ca + Mg) ratio approached 2.2 by March 26, which coincided closely with the average tetany date (March 29). Serum Mg averaged 1.97, 3.58 and 2.06 mg/dl for treatments 1, 2 and 3, respectively, on February 15 before turning cows to pasture. There were no treatment differences for serum Ca, P and K during the experiment. Eight cows exhibited symptoms of grass tetany (collapse) on an average date of March 29. Winter Mg supplementation provided little long-term protection against hypomagnesemia after turning cows to tetanigenic pasture, indicating that cows must have a supplemental source of Mg during this critical period.

Animals↗

Increase in noradrenaline-synthesizing enzyme activity in medulla oblongata in Parkinson's disease.

Dopamine beta hydroxylase (DBH), The noradrenaline-synthesizing enzyme, and phenyl-ethanolamine-N-methyltransferase (PNMT), the adrenaline-synthesizing enzyme, were assayed in 18 areas of brain stem in eight cases of parkinsonian syndromes and of four age- and postmortem delay-matched controls. Dissection was performed by the "punch" technique and enzyme activities assayed by radiometric methods. No significant change was found for PNMT activity. DBH activity was significantly increased in the A2-C2 area of the medulla oblongata (including the nucleus tractus solitarius) in the cases of Parkinson's disease. The A2-C2 area is known to be implicated in the control of blood pressure in rats. These findings are discussed in relation to orthostatic hypotension and the influence of L-dopa therapy.

Aged↗

[Possible alteration of adrenergic neurons in the mesolimbic system of psychotics (author's transl)].

The activity of Phenylethanolamine-N-Methyltransferase (PNMT) was assayed in 12 brain structures in a group of 8 chronic psychotics, a sub-group of 5 schizophrenics and a group of 7 normal controls. It was found significant decreased in substantia perforata posterior and nucleus accumbens. These two structures belong to the meso-limbic system. These data, therefore, suggest an inhibition of PNMT and/or an alteration of putative adrenergic neurons in the meso-limbic system, in schizophrenia.

Humans↗

[Activities of enzymes synthesizing catecholamines in areas of the brain stem in sudden infant death].

In victims of Sudden Infant Death Syndrome, the activity of phenylethanolamine-N-methyltransferase was found to decrease in the nucleus retroambigu, the Kolliker-Fuse nucleus and the C 2 area of medulla, whereas a decrease in the dopamine-beta-hydroxylase activity was found only in the C 2 area. These results suggest a hypoactivity of adrenergic neurons in respiratory and cardiac centres in the Sudden Infant Death Syndrome.

Adrenergic Fibers↗