Self-sorting of isotropic cells.
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Animals↗
Biomedical subjects
N Goel
Publications and source records attributed to N Goel.
Relationship of circadian activity and social behaviors to reentrainment rates in diurnal Octodon degus (Rodentia).
Previous experiments with female Octodon degus showed that entrained males (donors) inhibited and entrained females accelerated female phase-shifters' teentrainment rates of temperature and activity rhythms following 6-h advances. In this experiment, entrained males were housed with other entrained males or with phase-shifting females (experiencing a phase advance of the light-dark (LD) cycle) and entrained females were housed with other entrained females or with phase-shifting females. Pairs separated by a barrier were videotaped for 66 h to identify the behavioral interactions that underlie the opposite effects of donor social cues on reentrainment rates in females. As reported previously, female phase-shifters housed with entrained females reentrained faster than those "housed with entrained males following 6-h advances. Despite accelerated reentrainment, the timing or total durations of general activity, sleep, cage investigation, barrier investigation, and scent marking did not differ between female phase-shifters housed with male or female donors, although all phase-shifters increased investigatory behaviors compared to entrained animals. In contrast, male and female donors housed with female phase-shifters increased the frequency of scent marking and the duration of cage and barrier investigation compared with those housed with entrained same-sex conspecifics. Changes in scent marking and sniffing investigation suggest olfactory cues, not alterations in sleep or other daily rhythms, supply the critical social influence necessary to alter reentrainment rates in females. Daily and/or specific phase increases in motor activity did not correlate with accelerated reentrainment. We suggest that the salience of social cues perceived by female phase-shifters, female phase-shifters' sensitivity to cues and/or the strength of emitted donor cues may contribute to differential responses of the circadian system in females housed with male vs. female donors.
Animals↗
High-risk human papillomavirus, tumor suppressor protein p53 and mitomycin-C in invasive squamous cell carcinoma cervix.
BACKGROUND: Clinical data relating to human papillomavirus (HPV) infection and p53 status in cervical cancer has been sparse and confusing. AIM: To evaluate high-risk HPV and expression of tumor suppressor protein p53 in squamous cell carcinoma of cervix and to assess response to mitomycin-C in neo-adjuvant chemotherapy. SETTING AND DESIGN: Teaching College Hospital; Gynecologic Oncology Unit and Department of Pathology. Prospective, randomized. MATERIALS AND METHODS: Expression of p53 protein was assessed, using immunohistochemistry with mouse monoclonal antibody in 30 consecutive patients undergoing radical hysterectomy or admitted for neo-adjuvant chemotherapy. Human papillomavirus DNA (HPV DNA) was assessed using hybrid capture II technology. Patients eligible for chemotherapy were randomized into vincristine, bleomycin and cisplatin (VBP) group and VBP with mitomycin C group. STATISTICAL ANALYSIS: Chi-square test, one-way ANOVA, Pearson's correlation; Mann-Whitney, McNemar and Fischer's exact tests were used for statistical analysis. RESULTS: All patients with cancer cervix were positive for high-risk HPV DNA having relative light units/cut off values ranging from 3.4-2389.21 (P value = 0.006). High viral load of high risk HPV DNA was seen in advanced stages (P = 0.05) and an association of viral load with tumor volume was also seen (r = 0.361, P = 0.05). Analysis of p53 protein in cervical carcinoma patient showed expression in 50% of cancer specimens (P value < 0.001). McNemar's and Fischer's exact test showed no change in p53 status post-chemotherapy; however 66% of stage II B patients in VBP-M group became operable. CONCLUSION: High-risk HPV was universally present in all cases of cancer cervix and viral load was associated with stage and tumor volume while p53 protein was expressed in 50% of cases suggesting deregulation. More studies using mitomycin-C in cervical cancer treatment protocols are needed.
Adolescent↗