Biomedical subjects

N Haskins

Publications and source records attributed to N Haskins.

6 recordsLinked to original sources

PubMed1999

High throughput liquid chromatography/mass spectrometric analyses using a novel multiplexed electrospray interface.

A novel four- channel multiplexed electrospray liquid chromatography interface is described. This device has been used to analyse both single components and mixtures by liquid chromatography/mass spectrometry (LC/MS) as well as synthetic samples prepared by automated procedures. These data provided unambiguous molecular weight assignments to both major components and synthetic by-products in these samples. In this work particular attention has also been paid to the elimination of interchannel crosstalk. Copyright 1999 John Wiley & Sons, Ltd.

Journal Article↗

PubMed1999

New mass spectrometric approaches to structural analysis in mixtures

Structural analysis of minor components in mixtures is a vital requirement in the development of any pharmaceutical compound. Mass spectrometry is uniquely able to give this kind of information on the trace amounts of material present as minor impurities in a drug substance. In this study we show that a combination of mass spectrometric analysers with different characteristics is an even more powerful approach with a higher chance of establishing a potential structure. In particular the advent of analysers capable of accurate mass measurement on small amounts of material has enabled structures to be proposed in situations where previously no real conclusions could be made. Copyright 1999 John Wiley & Sons, Ltd.

Journal Article↗

PubMed1998

Intensive nursing care of patients with a microvascular free flap after maxillofacial surgery.

This article provides an introduction to the care of patients following maxillofacial surgery, many of whom undergo the complexities of microvascular flap surgery and need careful nursing assessment in the postoperative period. A brief introduction to this surgery illustrates some of the potential reasons for admission to the intensive care unit. The nursing care is vital to maintain the survival of the flap, the details of which are discussed along with factors which potentially contribute to flap failure. Other considerations such as haemodynamic stability, postoperative complications, the importance of education and other options for postoperative management also form further points for discussion.

Critical Care↗

PubMed1997

Minimizing cationization effects in the analysis of complex mixtures of oligosaccharides.

We outline simple methodology for the rapid and selective analysis of 2-aminoacridone (2-AMAC) derivatised oligosaccharides by matrix-assisted laser desorption/ionization mass spectrometry. This involves the addition of small amounts of lithium chloride to the matrix before evaporation of solvent and crystallization. Signals mainly attributed to proton, sodium and potassium adducts are suppressed to a great extent, and a single signal due to (M + Li)+ is observed. This technique is rapid and is most useful for the direct analysis of complex glycan mixtures, after derivatization with 2-aminoacridone and without separation of the individual components.

Aminoacridines↗

PubMed1982

Structural studies of alfalfa roots infected with nodulation mutants of Rhizobium meliloti.

Alfalfa roots infected with four nodulation defective (Nod-) mutants of Rhizobium meliloti which were generated by transposon Tn5 mutagenesis were examined by light and electron microscopy. In one class of Nod- mutants, which we can nonreactive, the bacteria did not induce root hair curling or penetrate host cells. In a second class of Nod- mutants, which we call reactive, the bacteria induced some root hair curling and entered root epidermal cells, although no infection threads were formed. In addition, reactive Nod- mutants induced extensive root hair proliferation and hypertrophied roots. This study presents the details of the phenotype of the association between each mutant strain and alfalfa roots.

Medicago sativa↗

PubMedDate not supplied

Rhabdomyolysis and acute renal failure in intensive care.

The aetiology and pathophysiology of the complex clinical condition rhabdomyolysis are outlined here Rhabdomyolysis can be caused by a number of factors including excessive muscle activity, direct muscle injury, ischaemia and excesses of temperature Specific nursing care strategies, focusing on the pathophysiology of the condition, are proposed The priorities of care should centre on maintaining patient safety with monitoring of cardiovascular and respiratory systems and assessment of renal function

Acute Kidney Injury↗