Biomedical subjects

N Hawrylak

Publications and source records attributed to N Hawrylak.

11 recordsLinked to original sources

PubMed2004

Glial limitans elasticity subjacent to the supraoptic nucleus.

Two previous studies from our laboratory have indicated that the ventral glial limitans subjacent to the hypothalamic supraoptic nucleus (SON-VGL) undergoes a reversible thinning upon chronic activation of the magnocellular neuroendocrine cells (MNCs) of the supraoptic nucleus (SON). Numerous other studies have shown that MNC somata hypertrophy with activation. One aim of the current study was to understand better how SON-VGL thinning occurs. A second aim was to quantify overall changes of the MNC somata region due to cellular hypertrophy to compare relative changes in dimensions. Here, we undertook a light microscopic stereological investigation of the SON and the subjacent SON-VGL of Nissl stained material under basal and activated conditions. Astrocyte numbers in the underlying SON-VGL remained stable across hydration state as did the overall volume of the SON-VGL and dendritic zone reference area. How these data are consistent with our earlier observations of SON-VGL thinning was resolved by the finding of a highly significant, 30% increase in the mediolateral dimension of the SON-VGL in dehydrated rats. These observations fit well with previous work from our laboratory that demonstrates a reorientation of SON-VGL astrocytes, from vertical to horizontal, which occurs in the activated SON-VGL. We found a significant, approximately 54%, increase in the overall volume of the MNC region of the SON. No significant rostrocaudal lengthening of the SON was detected, although a trend was evident. All the observed changes reversed with rehydration. These data indicate that elasticity of the SON-VGL acts to accommodate the volume expansion of the MNCs and enables the SON-VGL to continue as an interface between the underlying cerebrospinal fluid in the subarachnoid space and the expanded SON above.

Adaptation, Physiological↗

PubMed2000

Neuronal-glial interactions and behaviour.

Both neurons and glia interact dynamically to enable information processing and behaviour. They have had increasingly intimate, numerous and differentiated associations during brain evolution. Radial glia form a scaffold for neuronal developmental migration and astrocytes enable later synapse elimination. Functionally syncytial glial cells are depolarised by elevated potassium to generate slow potential shifts that are quantitatively related to arousal, levels of motivation and accompany learning. Potassium stimulates astrocytic glycogenolysis and neuronal oxidative metabolism, the former of which is necessary for passive avoidance learning in chicks. Neurons oxidatively metabolise lactate/pyruvate derived from astrocytic glycolysis as their major energy source, stimulated by elevated glutamate. In astrocytes, noradrenaline activates both glycogenolysis and oxidative metabolism. Neuronal glutamate depends crucially on the supply of astrocytically derived glutamine. Released glutamate depolarises astrocytes and their handling of potassium and induces waves of elevated intracellular calcium. Serotonin causes astrocytic hyperpolarisation. Astrocytes alter their physical relationships with neurons to regulate neuronal communication in the hypothalamus during lactation, parturition and dehydration and in response to steroid hormones. There is also structural plasticity of astrocytes during learning in cortex and cerebellum.

Animals↗

PubMed1999

The surface density of glial fibrillary acidic protein immunopositive astrocytic processes in the rat supraoptic nucleus is reversibly altered by dehydration and rehydration.

Ultrastructural and immunohistochemical studies of the supraoptic nucleus (SON) have provided evidence that retraction and extension of astrocytic processes from between magnocellular neuroendocrine cells (MNCs) likely plays a role in the release of oxytocin, and/or vasopressin, that accompanies parturition, lactation and dehydration. The present study estimates the surface density (Sv) of glial fibrillary acidic protein (GFAP)-immunoreactivity, predominantly in astrocytic processes, in the SON of normally hydrated, dehydrated and rehydrated rats. The Sv of GFAP processes in dehydrated rats was significantly reduced compared with control levels. Rehydration returned Sv to control levels. The reversible reduction in Sv indicates that the previously observed reduction in optical density is due to a rearrangement of astrocyte processes in the SON which occurs at the same time as the selective functional activation of MNCs.

Animals↗

PubMed1998

Dehydration and rehydration selectively and reversibly alter glial fibrillary acidic protein immunoreactivity in the rat supraoptic nucleus and subjacent glial limitans.

Ultrastructural studies of the supraoptic nucleus (SON) of the hypothalamus suggest that an active retraction and extension of astrocytic processes (structural plasticity) from between magnocellular neuroendocrine neurons plays a role in the release of oxytocin, vasopressin, or both peptides that accompanies parturition, lactation, and dehydration. In support of this, Salm et al. (1985) previously demonstrated a lactation-associated reduction in immunoreactive glial fibrillary acidic protein (GFAP), an astrocyte-specific cytoskeletal constituent. To determine if similar changes occur in response to dehydration, and if they are reversible, the present study examined GFAP-immunoreactivity (IR) in the SON under various hydration states. Rats were dehydrated for 7 days by substitution of drinking water with 2% saline (n = 3), or dehydrated for 7 days followed by 7 days of rehydration (n = 3). A control group (n = 3) with free access to tap water was used for comparisons. The optical density of GFAP-IR was obtained from the SON, globus pallidus, and lateral hypothalamic regions. The areas of the ventral glial limitans subjacent to the SON (SON-VGL) and of linearly equivalent segments of glial limitans more distant from the SON were also determined. Dehydration resulted in a significant reduction in GFAP-IR in the SON compared to control and rehydrated levels. We also found that the area of the SON-VGL was significantly larger than that of linearly equivalent segments of glial limitans elsewhere and that it was significantly reduced in dehydrated rats, returning to control levels with rehydration. GFAP-IR and glial limitans thickness in regions unrelated to body fluid homeostasis lateral to the SON, overlying to dorsal cortex, and subjacent to the optic chiasm were not significantly changed by hydration state. These results are similar to the changes of GFAP-IR reported for lactating rats and provide further evidence for a role of structural plasticity of astrocytes in events surrounding the selective functional activation of local neurons.

Animals↗

PubMed1996

Rapid laminar-dependent changes in GFAP immunoreactive astrocytes in the visual cortex of rats reared in a complex environment.

Neuronal changes in the visual cortex have previously been found to occur within days of housing weanling rats in a complex environment (EC) compared to rats housed in standard laboratory cages (IC). In contrast, layer IV astrocytes immunostained for glial fibrillary acidic protein (GFAP) have been found to be slow to change. Recent quantitative analysis has shown the surface density of GFAP immunoreactive (GFAP-IR) astrocytes in young rats to be significantly lower in layer IV in comparison to layer II/III. In the present study, the analysis of experience effects on GFAP-IR astrocytes was extended to include layer II/III as well as layer IV of EC and IC rats. The surface density of GFAP-IR processes was found to be significantly increased within layer II/III after 4-10 days of EC rearing in comparison to IC rats. Consistent with previous findings, housing condition did not significantly affect GFAP-IR within layer IV during these early time points. It is possible that GFAP immunocytochemistry is not a sensitive means of detecting experience-induced early changes in astrocytes within layer IV of weanling rats. The rapid astrocytic changes detected in layer II/III are suggestive of a close relationship between astrocytic plasticity and experience-induced synaptic plasticity.

Animals↗

PubMed1995

Expression of DMAP-45R in the rat visual cortex is modulated by visual experience.

Effects of visual experience upon expression of a developmentally regulated microtubule-associated protein (MAP) were studied in the visual cortex of monocularly deprived rats. The antibody Drosophila MAP-45 (DMAP-45) recognizes proteins in the developing ventral nerve cord of Drosophila and in rat brain. Monocular deprivation from day 12, before eye opening, to day 80 reduced the number of DMAP-45 immunoreactive layer V pyramidal cell apical dendrites in the monocular segment (Oc1M) of the visual cortex contralateral to the deprived eye. No significant visual deprivation effects were seen in the binocular segment (Oc1B). Immunoreactivity was restored to control levels in Oc1M of rats in which the monocular sutures were removed at day 75, subsequently allowing 5 days of exposure to light. These results indicate potential involvement of this MAP in experience-dependent structural plasticity.

Animals↗

PubMed1995

Monocular deprivation alters the morphology of glial fibrillary acidic protein-immunoreactive astrocytes in the rat visual cortex.

Monocular deprivation was used to examine the experience-dependent structural plasticity of astrocytes in Oc1M and Oc1B visual cortex of young and adult rats. Stereological techniques were employed to assess the numerical density (Nv) of cells and surface density (Sv) of processes immunoreactive for glial fibrillary acidic protein in laminae II/III, IV, V and VI in the hemisphere opposite the deprived eye. In one group of pups eyelids were sutured on postnatal day 12 (P12) and maintained until P80 (MD), while a second group had the sutures removed at P75 followed by 5 days of light exposure (MD + L). An unoperated light experienced group was used for comparisons (L). The Sv of astrocytic processes in lamina IV but not laminae II/III, V and VI was significantly decreased in the MD group. The ratio of Sv to the Nv of neurons, an estimate of the amount of astrocytic membrane per neuron, was also significantly decreased in layer IV. The Nv of astrocytes was not significantly different among the three groups. In adults that were monocularly deprived for 5, 10 and 30 days the Nv of astrocytes and Sv of their processes were not significantly altered in layer IV. There was however an increase in the Nv of all types of glial cells combined in layer IV following 10 and 30 days. These results indicate that the structure of astrocytes is influenced by visual experience during development whereas merely altering the level of visually-driven activity in the adult was not sufficient to induce astrocytic structural change.

Age Factors↗

PubMed1995

Morphogenesis in memory formation: synaptic and cellular mechanisms.

We review some of the evidence for structural changes in synapses in response to environmental stimulation. These include changes in synapse number, in distribution of presynaptic vesicles, in synaptic bouton size, and complex changes in the shape and size of synaptic contact zones. Increased numbers of postsynaptic polyribosomal aggregates (PRA) are correlated histologically with developmental plasticity. We discuss the role that dendritically targeted mRNAs and polyribosomes might play in providing rapid, localized synthesis of proteins necessary for structural change. Using synaptoneurosomes, we have demonstrated that depolarization leads to a rapid (1-2 min) increase in PRA and in [35S]methionine incorporation into polypeptides. We have shown that this process is initiated by metabotropic glutamate receptors, which trigger phosphatidyl inositol hydrolysis, leading to release of internal Ca2+ stores and activation of protein kinase C. Entry of external Ca2+, however, seems to downregulate polyribosomal aggregation, via a calmodulin-dependent mechanism, suggesting that translation may be controlled by interaction of ionotropic receptors, voltage-dependent calcium channels, and metabotropic receptors.

Animals↗

PubMed1993

Astrocytic and synaptic response to kindling in hippocampal subfield CA1. I. Synaptogenesis in response to kindling in vitro.

Early morphological events associated with the genesis of epileptiform activity are essentially unknown, despite significant progress on morphological correlates of potentially related plastic neural phenomena. Hippocampal area CA1 shows the capacity to generate epileptiform bursting activity after certain patterns of electrical stimulation. Using an in vitro slice kindling preparation, we found increases in the numbers (areal densities) of shaft and sessile spine synapses in hippocampal subfield CA1 within minutes following the establishment of stable afterdischarges. These data strongly suggest that synaptogenesis is associated with the early stages of epilepsy formation.

Animals↗

PubMed1993

Astrocytic and synaptic response to kindling in hippocampal subfield CA1. II. Synaptogenesis and astrocytic process increases to in vivo kindling.

Astrocytic glia are important for maintaining synaptic function during physiological activity. Recent hypotheses concerning epilepsy suggest a role for astrocytes in the control of neuronal excitability and in pathogenesis. This report provides morphological evidence that the periodic electrical stimulation used in the kindling model of epilepsy induces astrocytic hypertrophy and an increase in shaft synapse density in the CA1 region of the hippocampus. The Schaffer collateral pathway in the stratum radiatum of CA1 of five pairs of rats was kindled in vivo. Control animals received the same number of stimulations at a lower intensity and frequency. The animals were killed 24-48 h after reaching the criterion of five generalized seizures, and the brains were examined by electron microscopy. Kindling produced a 37% and 33% increase in the volume fraction of astrocytic processes in the middle and distal portions, respectively, of the s. radiatum in CA1. In the same tissue, the number (areal density) of shaft synapses was increased 25% in the s. radiatum of animals exhibiting generalized seizures. On the other hand, the areal density of degenerating synapses in both kindled and control animals was low and not significantly different. These results suggest that both synaptogenesis and hypertrophy of astrocytes contribute to an early stage of epileptogenesis when degenerative changes of the sort that might induce gliosis were not prominent in the tissue under study.

Animals↗

PubMed1993

Nuclear magnetic resonance (NMR) imaging of iron oxide-labeled neural transplants.

Nuclear magnetic resonance (NMR) imaging in vivo of adult rat brains was used to observe the fate of iron oxide-labeled intracerebral neural grafts. The host animals received grafts of fetal (E17-E18) rat tissue prepared as cell suspensions and labeled by incubation with reconstituted Sendai viral envelopes containing iron oxide particles. Control studies were performed in animals following surgical trauma alone or transplantation of unlabeled cell suspensions. In vivo NMR imaging (either two-dimensional Fourier transform or three-dimensional Fourier transform) was performed once on each animal between 5 and 60 days postsurgery. The NMR images of the host brains containing the labeled cells showed sufficient anatomical detail for the identification of the major brain structures. The graft sites were seen in the T2-weighted NMR images as dark regions (low-intensity signal) in the cortex. Histochemical staining for ferric iron (prussian blue stain) demonstrated the presence of numerous prussian blue-positive cells in tissue sections corresponding to the dark regions in the NMR images. Surviving prussian blue-positive cells with neuron-like morphology were relatively more numerous 10 days after transplantation than at 1-2 months postgrafting. The NMR images and immunohistochemical and histochemical staining of hosts containing labeled cells suggest cell migration of astrocytes and macrophages up to 2 mm away from the graft sites. The migrating cells were primarily along fiber tracts in the host white matter. Prussian blue deposits were also present within the brains of the control animals, but the NMR images from the same animals did not contain distinct dark regions like those in the images of brains which had received labeled cell transplants. These results demonstrate that NMR imaging can be used to study cell survival and migration following neural grafting procedures.

Animals↗