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N Hejtmánková

Publications and source records attributed to N Hejtmánková.

11 recordsLinked to original sources
PubMed1992

Genetics of dermatophytes.

By the end of 60s a team of scientists has started at Medical Faculty of Olomouc the research of micromycetes pathogenic for both the humans and animals. In this study, the appropriate results are summarized as concerned with the genetics of dermatophytes. Among these results, those significant in deeper assessing the biology of dermatophytes and etiopathogenesis of dermatophytoses have been selected. In our opinion, they may be of interest for the successors in the direction mentioned. Here are also formulated certain open problems and the applicative outputs are traced, for example, those concerning with vaccines. The approaches used, even unusual in the medical mycology from a traditional scope, are believed to prove the convenience of genetic methods when researching mycopathogens. We regret to miss at the edition of this publication the late founder of the team, RNDr. Nora Hejtmánková, CSc. which had the greatest contribution to the Chapters on the karyology and variability of dermatophytes as well as to the hybridization analysis of Trichophyton mentagrophytes complex.

Arthrodermataceae↗
PubMed1989

Hybridization and sexual stimulation in Trichophyton mentagrophytes.

Mating and sexual stimulation tests applied to 132 strains of this dermatophyte isolated in Czechoslovakia revealed among them strains of Arthroderma benhamiae (40 strains of the + mating type, one of the - mating type) and A. vanbreuseghemii (three strains of the + type, seven of the - type). No dependence was found concerning the anamorphic variety (T. mentagrophytes var. granulosum, var. interdigitale, var. mentagrophytes, var. quinckeanum), teleomorphic species, mating type and the clinical localization of dermatophytosis. Plausible reasons of different frequencies of the mating types are discussed.

Crosses, Genetic↗
PubMed1989

Teleomorphs and mating types in Trichophyton mentagrophytes complex.

In the set of 132 strains of Trichophyton mentagrophytes isolated from humans (129 strains) and animals (3 strains) with dermatophytosis in Czechoslovakia, 41 strains were classified as Arthroderma benhamiae. Out of them, 40 strains were of mating type + and one strain of type -. Ten strains were A. vanbreuseghemii, including 3 strains of mating type + and 7 strains of type -. Other strains studied did not form any fertile cleistothecia during crossing. Some strains of A. benhamiae developed defective cleistothecia with rare asci or without ascospores. From conidia of two strains (T. mentagrophytes var. mentagrophytes and T.m. var. quinckeanum) two morphological variants (granular and fluffy) were cultivated. Both variants were A. benhamiae type + and pathogenic. The determination of mating types using the test of sexual stimulation with Arthroderma simii and hybridization test with the reference strains of A. benhamiae and A. vanbreuseghemii gave identical results. No relationships between the variety of anamorphous state (T. mentagrophytes var. mentagrophytes, var. granulosum, var. interdigitale, var. quinckeanum), teleomorph, mating type, localization of dermatophytosis, and pathogenicity for guinea pig were found. The reasons for unequal distribution of mating types in clinical isolates of various dermatophytes are discussed.

Humans↗
PubMed1989

[Fluorescence microscopy detection of mycopathogens using Rylux BSU].

Rylux BSU (Synthesia, Pardubice--Semtín), a derivative of diaminostilbendisulphonic acid, has a good affinity to chitin of fungal cell walls as well as to cellulose of algae. A histologic method using this compound for detection of fungi in dermatology is presented accompanied with results of a quick diagnostic procedure. Pathogenic fungi were stained with a 2 per cent solution of Rylux BSU in phosphate buffer (pH 7.3) or in a 2 per cent aqueous solution of NaOH with an admixture of 10 per cent dimethylsulphoxide. A yellow green fluorescence of the cell walls of fungi (tested on 52 species) and algae (2 species) was evoked by incident blue light (max. filter transmission for 405 nm) combined with a barrier filter (max. 578 nm). Fluorescence was not fading and remained stable in Canada balsam. Moreover, the method could be applied on sections previously stained (HE, Weigert, Giemsa, KOH-preparations) because of more information offered by a combined procedure.

Benzenesulfonates↗
PubMed1977

Distribution of nuclei in macroconidia of Microsporum gypseum.

The distribution of nuclei in macroconidia was investigated in 20 strains of the dermatophyte Microsporum gypseum by fluorescence microscopy. The macroconidia were characterized by the right-left and the central-marginal symmetry indices. In 18 strains the significant majority of the nuclei were situated in the basal part of the macroconidium, the remaining 2 strains showed a more or less uniform distribution of nuclei. All strains had higher numbers of nuclei in the central part of the macroconidium as compared with the periphery.

Cell Nucleus↗