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Biomedical subjects

N J Basson

Publications and source records attributed to N J Basson.

4 recordsLinked to original sources

Orthodontic microabrasive reproximation.

Reproximation (enamel stripping) is described in the literature as a clinical procedure for correction of tooth size deviations. The objective of this study was to qualitatively assess, by means of scanning electron microscopy, (1) the differences exhibited on enamel with mechanical and chemical methods of stripping, and (2) the effect of a synthetic calcifying solution on the etched enamel. Part 1. Sixty human anterior teeth (10 complete sets) that were previously stored in 70% ethanol were subdivided into groups I and II. The teeth in group I were divided into five sets of six teeth mounted in a plaster block in the anterior arch form. Each set was stripped with one of the following mechanical abrasive methods: garnet disks, tungsten carbide and fine diamonds burs, coarse and fine diamond burs, diamond wheel and 3M strips, diamond-coated metal and 3M strips. The teeth in group II were similarly treated, except each set was subjected to a further microabrasive chemical stripping with 37% phosphoric acid used in conjunction with 3M strips. The teeth were then prepared for scanning electron microscopy, viewed, and photographed under magnification. Part 2. Ten human central incisor teeth were etched and used to study the effect of remineralization solutions at various time intervals. The results showed that teeth stripped by routine mechanical abrasive methods exhibited deep furrows and roughness. The teeth that received mechanical and chemical abrasive treatments showed a flattened, etched surface free of furrows. These etched surfaces showed marked crystal growth at 5 and 10 hours after remineralization suggesting the possibility of repair of the chemically altered enamel surface.

Acid Etching, Dental

Growth interaction between Candida albicans and Streptococcus salivarius: in vitro and in vivo studies.

Suppression of Candida albicans in the mouth by oral flora has been proposed as one of the mechanisms preventing candidal overgrowth. According to Liljemark and Gibbons (1973), Streptococcus salivarius plays a significant role in this process. The aim of this investigation was to study the growth interaction between C. albicans and S. salivarius in vitro and in vivo. An aerobic continuous-flow system was used for the in vitro study. Pure and mixed cultures of C. albicans (NCPF 3118) and S. salivarius (NCTC 8618) were inoculated into a buffered medium containing either 0.1 per cent or 0.001 per cent glucose concentrations and incubated at 37 degrees C for 55 hours. Two in vivo investigations were undertaken using inbred germfree C3H mice. In the first, mice were exposed to a mixed suspension of S. salivarius and C. albicans for 48 hours. In the second the mice were exposed to S. salivarius for 48 hours. Fourteen days later they were contaminated with C. albicans. A comparison of growth curves showed no growth inhibition between the species. The in vivo studies showed that oral lesions from candidal infestation occurred in all mice. We were therefore unable to demonstrate in vitro or in vivo suppression of C. albicans in the presence of S. salivarius.

Animals

Evaluation of inbred germ-free Fischer 344 albino rats as an experimental model for oral candidiasis.

Inbred germ-free Fischer 344 albino rats were evaluated as models for experimental candidiasis in order to investigate bacterial interaction with Candida albicans. Female rats were exposed to C. albicans in their drinking water and killed at intervals from 2 to 22 days after initial contact with the contaminant. C. albicans was cultured from their mouths from day 2 but from day 12 the number of colonies decreased. From day 2 to 9 all rats showed oral histological signs of candidal infestation, but after 9 days the number declined to 3 out of 9 at 22 days. The dorsal surface of the tongue was the best histological indicator of candidal infestation. All the rats had tongue lesions from day 4 to 9, and from day 6 there was also a concomitant localized loss of filiform papillae. The number of rats with all forms of tongue involvement also decreased after 9 days with only 3 out of 9 affected at 22 days. It is concluded that Fischer 344 inbred germ-free rats can be used on a limited scale as a model for candidiasis and bacterial interaction with C. albicans, the dorsal surface of the tongue would be the best site for studying candidal experimental lesions and it is probable that better results can be achieved with complete standardization of contamination and preparation procedures.

Animals