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N J Larsen

Publications and source records attributed to N J Larsen.

25 records · Page 2Linked to original sources
PubMed1996

Seven genes from human chromosome 18 map to chromosome 24 in the bovine.

Bovine sequence tagged sites (STSs) were developed for seven genes and used for synteny mapping with a hybrid bovine x rodent cell line panel. The genes were thymidylate synthase (TYMS), pituitary adenylate cyclase activating peptide (ADCYAP1), and melanocortin-2 receptor (MC2R) from the short arm of human chromosome (HSA) 18 and N-cadherin (CDH2), transthyretin (TTR), gastrin-releasing peptide (GRP), and plasminogen activator inhibitor 2 (PAI2) from the long arm of HSA 18. Primers for these genes were designed with human, ovine, or bovine sequences aligned with a sequence from a second species. The bovine PCR product was cloned, and the fragment was sequenced to verify that the homologous gene was indeed amplified. A second set of bovine-specific PCR primers were developed for each gene from these sequences. These STSs were used for synteny mapping, and all seven genes were syntenic with markers of bovine chromosome (BTA) 24. The concordance with BTA 24 was at least 96.5% for all genes.

Animals↗
PubMed1993

Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle.

Fifty-eight calves of both sexes from lines of Red Danish dairy breed selected for high (n = 36) and low (n = 22) milk fat production, and 32 heifers from lines of Norwegian Red dairy breed selected for high (n = 16) and low (n = 16) milk fat yield were typed for two previously reported restriction fragment length polymorphisms (RFLPs) in the growth hormone gene. The RFLPs are consistent with: (1) an insertion(I)/deletion(D) of approximately 0.9 kb in the 3'-region of the growth hormone gene and (2) a polymorphic MspI(+/-) site in the third intron. A traditional RFLP procedure was used for typing the I/D polymorphism and a polymerase chain reaction (PCR) procedure was developed for typing the MspI polymorphism. Only the I-MspI(+) and D-MspI(-) haplotypes were found. In the Red Danish lines the frequency of D-MspI(-) haplotype was 0.28 in high line and 0.05 in low line calves, this difference was significant (P < 0.01). The corresponding frequencies in the Norwegian Red lines were 0.09 in the high line and 0.0 in the low line. Attempts to screen for RFLPs in the growth hormone receptor gene and in the insulin-like growth factor-I gene were unsuccessful.

Alleles↗
PubMed1991

Restriction fragment length polymorphisms at the growth hormone gene in pigs.

Pigs from four Danish and two Swedish populations were examined for restriction fragment length polymorphism (RFLP) at the growth hormone (GH) gene. Polymorphism was detected with the restriction enzymes DraI and TaqI. A comparison of the Danish populations showed significant differences among their allelic frequencies.

Alleles↗