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Biomedical subjects

N J Pound

Publications and source records attributed to N J Pound.

8 recordsLinked to original sources
PubMed1975

Simultaneous determination of phenylbutazone and oxyphenbutazone in plasma by high-speed liquid chromatography.

A sensitive, specific, high-speed liquid chromatographic procedure is described for the simultaneous determination of phenylbutazone and its metabolite, oxyphenbutazone, in plasma. Acidified plasma is partitioned with cyclohexane-ether (1:1) containing the 2,4-dinitrophenylhydrazone of 3,4-dimethoxybenzaldehyde as an internal standard. The organic extract is reduced to dryness, the resulting residue is redissolved in chloroform, and aliquots of this solution are chromatographed on an adsorption column, using a mobile phase of 0.002% acetic acid and 23.0% tetrahydrofuran in n-hexane at 35 degrees. Use of a UV detector permits quantitative analysis of samples containing less than 0.25 mug/ml of phenylbutazone or oxyphenbutazone.

Chromatography↗
PubMed1975

Simultaneous high-speed liquid chromatographic determination of tetracycline and rolitetracycline in rolitetracycline formulations.

A rapid, precise high-speed liquid chromatographic procedure for the simultaneous determination of tetracycline and rolitetracycline in rolitetracycline formulations is described. Samples are dissolved in water, chilled to 0 degrees, and chromatographed on a pellicular cation-exchange resin, The specificity of this method represents a significant improvement over present analytical procedures, which fail to differentiate between rolitetracycline and its hydrolysis product, tetracycline, in these formulations.

Biological Assay↗
PubMed1973

Separation and detection of tetracyclines by high-speed liquid chromatography.

The high-speed liquid chromatographic behavior of 15 tetracyclines on anion- and cation-exchange columns is described and illustrated by typical separations of a number of tetracycline derivatives on several systems, as well as by the resolution of tetracycline from its degradation products epi-, epianhydro-, and anhydrotetracycline. These serve as a basis for a rapid, convenient, and precise method for the direct detection of the tetracycline antibiotics and their degradation products.

Chromatography, Ion Exchange↗