How to safely clean surgical instruments.
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Biomedical subjects
Publications and source records attributed to N J Schneider.
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In three subject-paced experiments we evaluated reading patterns at the word, line, and sentence level for fast and slow readers. A moving-window method was used to collect word reading times for natural texts. At the word level, reading times of word N were influenced by features of word N-1 for fast readers but not for slow readers. The lag effect exhibited by fast readers indicates that they continue to process a word when it is no longer in view, thus limiting the notion of immediate processing. Contrary to our initial expectation that fast readers would process only a single new argument from a sentence, whereas slow readers would process several new arguments, we found that both reader groups adopted a many-argument strategy. However, fast and slow readers differed in terms of the text units (lines vs. sentences) defining the new-argument effects: Fast readers exhibited greater new-argument effects relative to lines, whereas slow readers exhibited greater new-argument effects relative to sentences. Specifically, slow readers integrated the new arguments primarily at the end of the sentence, whereas fast readers did so at line boundaries. These results are discussed in terms of a buffer-and-integrate model of reading comprehension.
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From 8 August through 5 December 1977, 110 laboratory-documented cases (78 confirmed, 32 presumptive) of St. Louis encephalitis (SLE) occurred in 20 counties throughout central-south Florida. Most cases were initially identified through an active surveillance system involving repeated contact of hospital infection control officers. This outbreak had three characteristics that are unusual for SLE epidemics in the eastern United States: it lacked an urban focus, a disproportionate number of cases affected young males, and the epidemic period extended into December. There was no significant difference between attack rates for blacks and whites. Males were significantly more often affected than females. Because of the time required for laboratory confirmation of cases, most outbreak control measures were initiated after the outbreak was declining. Reporting of clinically suspected SLE cases to initiate epidemic mosquito control should improve the timeliness of control measures in future outbreaks.
Hemoglobin Tampa was detected in a 6-year-old male caucasian who is homozygous for this variant hemoglobin. The variant hemoglobin has an electrophoretic mobility between Hb F and Hb S on cellulose acetate (pH 8.5) and a mobility between Hb S and Hb C on citrate agar (pH 6.0). In acid buffer globin chain analysis revealed an abnormal beta chain with a mobility between the beta A and beta S chains, and in alkaline buffer the mobility of the chain was at the beta S position. Structural characterization of the variant beta chain indicates that aspartic acid is replaced with tyrosine at position 79, the site of a previously reported mutation, Asp replaced by Gly (Hb Hsi-Tsou). The clinical histories of the available family members including the homozygous propositus appear to be unremarkable.
Within 72 hours of visiting the Everglades National Park in south Florida, a 43-year-old man became ill with fever, malaise, myalgia, severe headache, pharyngitis, and enlarged, tender lymph nodes. Everglades (Venezuelan equine encephalomyelitis, subtype II) virus was isolated from a blood sample drawn from the patient five days after onset of symptoms.
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Of 21 antimicrobial agents tested in vitro, amikacin was the most predictably active against clinical isolates belonging to the Mycobacterium fortuitum complex; however, only 50% of strains studied were susceptible to clinically attainable concentrations of the drug.
Serum proteins of gray squirrels were electrophoretically separated into 7 fractions as compared with the 5 fractions obtained from human serum. The mobility of the albumin fraction was approximately the same for both human and squirrel serums. The prealbumin fraction did not vary significantly in the percentage for squirrels bled at different times of the year. Immunoelectrophoresis patterns showed squirrel serum had a small number of fractions with the same antigenic characteristics as human serum.
Six obligately anaerobic strains of Corynebacterium vaginale (Haemophilus vaginalis) have been isolated and their characteristics studied. The reactions of the anaerobic strains, as well as of facultative strains tested under anaerobic conditions, are similar to the reactions previously reported for this species.
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A procedure for the laboratory screening of hemoglobinopathies is described. The procedure employs hemoglobin electrophoresis followed by solubility testing to confirm presence of sickling hemoglobins. All initial screening tests are carried out on whole blood specimens collected on filter paper. The use of filter paper facilitates collection and results in considerable saving in time and mailing cost. The results of the first year screening confirm the findings of earlier studies that blood specimens collected on filter paper are satisfactory for electrophoresis and solubility tests. All tests are relatively easy to perform using readily available commercial reagents. The procedure is particularly suitable for public health laboratories in carrying out hemoglobinopathy screening programs.
The first reported case of hemoglobin S and C Harlem in an individual is described. The patient, a 35-yr-old female, had numerous crises during adolescence and early adulthood, but these occurred more infrequently as she grew older. Chemical evidence is presented for the characterization of both variant hemoglobins. The clinical course of this individual with Hb S in combination with Hb C Harlem appears to be similar to that for persons with sickle cell anemia.
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