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N K Christensen

Publications and source records attributed to N K Christensen.

7 recordsLinked to original sources

Incorporation of alpha- and beta-LNA (locked nucleic acid) monomers in oligodeoxynucleotides with polarity reversals.

The thymidine monomers of LNA with both alpha- and beta-configuration are incorporated with polarity reversals (i.e., with 3'-3' and 5'-5' junctions) in oligodeoxynucleotides with beta- and alpha-configuration, respectively. A 5'-O-phosphoramidite of the beta-LNA monomer is synthesised. Large destabilisations of duplexes with both complementary DNA and RNA are observed for oligodeoxynucleotides containing the alpha-LNA monomer, whereas a duplex with complementary RNA of an alpha-oligodeoxynucleotide containing the beta-LNA monomer is not destabilised.

Nucleic Acids↗

Standards for moderate thermal environments.

Recently two Draft International Standards dealing with specifications of the conditions for thermal comfort (ISO DIS 7730) and measurement procedures (ISO DIS 7726) have been approved by the International Stadardisation Organisation (ISO). The American Society of Heating, Refrigerating and Air Conditioning Engineers (ASHRAE) has made a standard with similar requirements for the thermal environment (ASHRAE, 1981). To verify the requirements, measurements of different thermal parameters have to be performed. Guidelines as to how and where to measure are also given in the standards. The present paper deals mainly with the requirements and measurements that are relevant for moderate thermal environments in places of residence, offices, hospitals and light industry. For evaluation of very hot or very cold surroundings, other methods are required. Only measurements of parameters that influence the perception of the thermal surroundings are included.

Journal Article↗

Diet-related knowledge, skill, and adherence among children with insulin-dependent diabetes mellitus.

The dietary adherence of a sample of 97 patients with insulin-dependent diabetes mellitus has been quantitatively described. Now, in an effort to understand the poor dietary adherence found, diet-related knowledge and skill and their relationship to dietary adherence among 90 diabetic children have been studied. Diet-related competencies assessed were the ability to: (1) recall the personal diet plan, (2) correctly fill one's plate from a buffet, with the diet plan in hand, and (3) choose an appropriate meal from a restaurant menu. Adherence to the diet was assessed by unobtrusive observations at meals. Error rates on the three tests of knowledge and skill were .21, .28, and .51, respectively. The mean error rate at mealtime was .35, only slightly higher than the error rate for filling a plate when the children knew they were being tested. In a multiple regression analysis, age (r = .37) and sex (R = .48) were associated with adherence to the diet plan. Ability to choose correctly from a menu, duration of diabetes, and ability to correctly fill a plate from a buffet also entered the regression equation (R = .54). The data suggest that many children did not possess the knowledge and skill required for good dietary adherence, regardless of motivation. Health educators may underestimate the complexity of the behaviors expected of chronically ill patients.

Adolescent↗

Synthesis and evaluation of alpha-LNA.

Three different synthetic routes to the alpha-configured LNA thymine monomer starting from D-allose or D-arabinose were investigated. The introduction of one or four alpha-LNA monomers into alpha-DNA had a destabilizing effect on the duplexes. However, a fully modified alpha-LNA sequence displayed strong recognition of complementary RNA, but no transition with DNA.

Arabinose↗

Quantitative assessment of dietary adherence in patients with insulin-dependent diabetes mellitus.

Research on the education of diabetic patients in diet management suffers from lack of an adequate method for describing patients' dietary behavior. In this report, a method is proposed for the quantitative assessment of dietary adherence in patients with IDDM. The method relies on comparisons between individualized diet plans and actual consumption as reflected by 24-h diet recalls. Data are presented that suggest this method has reliability and validity. In a sample of 97 patients with IDDM, nearly two-thirds adhered to the number and timing of planned feedings, while only about 10% of patients adhered to planned exchanges, 90% of the time. The average patient added or deleted one exchange for every four exchanges in the diet plan.

Adolescent↗